cDNA Clone AS160

• Synonyms: TBC1D4
• Plasmid: pGEX6P-3-AS160 1-916
• Parent Plasmid: pGEX6P3
• Expressed: GST AS160 1-916
• Genbank: NM_014832
• Tag: GST
• Species: Human

Sequence of Insert

ggatccgccaccatggagccgcccagctgcattcaggatgagccgttcccgcaccccctggagcccgagccgggcgtctcagctcagcccggccccgggaagccaagcgataagcggttccggctgtggtacgttggggggtcgtgcctggaccacaggaccacgctgcctatgctgccctggctcatggccgagatccgcaggcgcagccagaagcccgaggcgggcggctgcggggcgccggcggcccgagaggtgatcctggtgctcagcgcgcccttcctgcgttgcgtccccgcgccgggcgctggggcctcggggggcactagtccgtcggccacgcagcccaacccggcggtattcatcttcgagcacaaggcgcagcatatctcgcgcttcatccacaacagccacgacctcacctactttgcctacctgatcaaggcgcagcccgacgaccccgagtcgcagatggcctgccacgttttccgcgccacagaccccagccaggttcctgatgttattagcagcataaggcaattatctaaagcggccatgaaagaggatgccaaacccagcaaagataatgaggacgccttttacaactctcag
aagttcgaagtcctgtactgtggaaaggtgaccgtgacccacaagaaggc
cccctcaagcctcatcgatgactgcatggagaagttcagcctgcacgaac
agcagcgcctgaagatccaaggcgagcagcgcggtccggacccaggagag
gacctggctgacttggaggtggtggtgcccgggtcccccggagactgcct
gccggaggaggctgacggcaccgacacccaccttggcttacctgccgggg
ccagccagcctgccctgaccagctctcgggtctgcttccctgagcggatt
ttggaagattctggctttgatgagcagcaggagtttcggtctcggtgcag
cagtgtcaccggcgtgcaacggagagttcacgagggcagccagaaatccc
agccgcgacggagacacgcgagcgcacccagtcacgtccagccctcggac
tcggagaagaacaggaccatgctcttccaggttgggcgatttgagattaa
ccttatcagtccagacactaaatcagttgtgctagaaaagaattttaaag
atatctccccttgttctcagggtataaagcatgtggatcactttggcttt
atctgccgggagtctccagagcctggacttagccagtatatttgttatgt
attccagtgtgccagcgaatctctggttgatgaggtaatgctgactctga
aacaggccttcagtacggcggctgccctgcagagtgccaagacgcagatt
aaactgtgtgaggcctgcccgatgcactctttgcataagctctgtgaaag
gattgaaggtctctacccaccaagagccaagctggtgatacagaggcatc
tctcatcactgacagataatgagcaagctgacatctttgaaagagttcag
aaaatgaagccagtcagtgaccaggaagaaaatgaacttgtgattttaca
cctgaggcagctgtgtgaagccaagcagaagacacacgtgcacatcgggg
aaggccctgctactatttcaaatagtacaatcccagaaaatgcaacaagc
agtggaaggttcaaacttgacattctgaaaaataaagctaagagatcctt
aactagctccctggaaaatatcttctcaaggggagctaacagaatgagag
gtcggcttggaagtgtggacagttttgaacggtccaacagtcttgcttca
gagaaggactactcaccaggggattctccaccagggacaccgccagcgtc
cccaccgtcctcagcttggcaaacgtttcccgaagaggattccgactccc
cgcagtttcgaagacgggcacacacgttcagccacccaccttcaagcaca
aagagaaagctgaatttgcaggatgggagggctcagggtgtgcgttcccc
tctgctgaggcagagctccagtgaacagtgcagtgatggagaagggagaa
aaaggacctcatctacctgcagcaatgagtccctaagtgtgggaggaacc
tctgtcactcctcgccggatctcctggcggcagcgcattttcctcagggt
tgcttctcccatgaacaaatctccctcagcaatgcaacagcaagatggat
tggacaggaacgagctgctgccactgtcccccctctctccaaccatggag
gaggaaccgctggttatattcctgtctggggaggatgacccagaaaagat
tgaagaaagaaagaaatcaaaagaactgaggagcttgtggagaaaagcta
tacaccaacaaatcttgttacttcgaatggaaaaagaaaaccagaaactt
gaagcaagcagagatgaactccagtccagaaaagttaaattagactatga
agaagttggtgcatgtcagaaagaggtcttaataacttgggataagaagt
tgttaaactgcagagctaaaatcagatgtgatatggaagatattcatact
cttcttaaagaaggagttcccaaaagtcgacgaggagaaatttggcagtt
tctggctttacagtaccgactcagacacagattgcctaataaacaacagc
ctcctgacatatcctataaggaacttttgaggcagctcactgctcagcag
catgcgattcttgtggatttaggaaggacgtttcctactcacccttacgaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSATMEPPSCIQDEPFPHPLE
PEPGVSAQPGPGKPSDKRFRLWYVGGSCLDHRTTLPMLPWLMAEIRRRSQ
KPEAGGCGAPAAREVILVLSAPFLRCVPAPGAGASGGTSPSATQPNPAVF
IFEHKAQHISRFIHNSHDLTYFAYLIKAQPDDPESQMACHVFRATDPSQV
PDVISSIRQLSKAAMKEDAKPSKDNEDAFYNSQKFEVLYCGKVTVTHKKA
PSSLIDDCMEKFSLHEQQRLKIQGEQRGPDPGEDLADLEVVVPGSPGDCL
PEEADGTDTHLGLPAGASQPALTSSRVCFPERILEDSGFDEQQEFRSRCS
SVTGVQRRVHEGSQKSQPRRRHASAPSHVQPSDSEKNRTMLFQVGRFEIN
LISPDTKSVVLEKNFKDISPCSQGIKHVDHFGFICRESPEPGLSQYICYV
FQCASESLVDEVMLTLKQAFSTAAALQSAKTQIKLCEACPMHSLHKLCER
IEGLYPPRAKLVIQRHLSSLTDNEQADIFERVQKMKPVSDQEENELVILH
LRQLCEAKQKTHVHIGEGPATISNSTIPENATSSGRFKLDILKNKAKRSL
TSSLENIFSRGANRMRGRLGSVDSFERSNSLASEKDYSPGDSPPGTPPAS
PPSSAWQTFPEEDSDSPQFRRRAHTFSHPPSSTKRKLNLQDGRAQGVRSP
LLRQSSSEQCSDGEGRKRTSSTCSNESLSVGGTSVTPRRISWRQRIFLRV
ASPMNKSPSAMQQQDGLDRNELLPLSPLSPTMEEEPLVIFLSGEDDPEKI
EERKKSKELRSLWRKAIHQQILLLRMEKENQKLEASRDELQSRKVKLDYE
EVGACQKEVLITWDKKLLNCRAKIRCDMEDIHTLLKEGVPKSRRGEIWQF
LALQYRLRHRLPNKQQPPDISYKELLRQLTAQQHAILVDLGRTFPTHPYE
FRVDSSGRIVTD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11525
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; Sequence differences in AS160 CDS compared with NM_014832:; T1159c (S387P); T1609g (S537A); Delta nt 2034-2222 (delta aa N679-S741) (The cloned sequence represents a splice variant lacking amino acids 679-741. The majority of ESTs in the NCBI database represent this form.); Delta nt 2594-2596 (delta aa G865)