cDNA Clone Nudel (NDEL1-PLSV)

• Plasmid: pGEX6P-Nudel
• Parent Plasmid: pGEX6P1
• Expressed: GST-Nudel
• Genbank: AY004871.1 GI:12043568
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGATGGTGAAGATATACCAGATTTTTCAAGTTTAAAGGAGGAAACTGCTTATTGGAAGGAACTTTCCTTGAAGTATAAGCAAAGCTTCCAGGAAGCTCGGGATGAGCTAGTTGAATTCCAGGAAGGAAGCAGAGAATTAGAAGCAGAGTTGGAGGCACAATTAGTACAGGCTGAACAAAGAAATAGAGACTTGCAGGCTGATAACCAAAGACTGAAATATGAAGTGGAGGCATTAAAGGAGAAGCTAGAGCATCAATATGCACAGAGCTATAAGCAGGTCTCAGTGTTAGAAGATGAT
TTAAGTCAGACTCGGGCCATTAAGGAGCAGTTGCATAAGTATGTGAGAGA
GCTGGAGCAGGCCAACGACGACCTGGAGCGAGCCAAAAGGGCAACAATAG
TTTCACTGGAAGACTTTGAACAAAGGCTAAACCAGGCCATTGAACGAAAT
GCATTTTTAGAAAGTGAACTTGATGAAAAGGAATCTTTGTTGGTCTCTGT
ACAGAGGTTAAAGGATGAAGCAAGAGATTTAAGGCAAGAACTAGCAGTTC
GGGAAAGACAACAGGAAGTAACTAGAAAGTCGGCTCCTAGCTCTCCAACT
CTAGACTGTGAAAAGATGGACTCCGCCGTCCAAGCATCACTTTCTTTGCC
AGCTACCCCTGTTGGCAAAGGAACGGAGAACACTTTTCCTTCACCGAAAG
CTATACCAAATGGTTTTGGTACCAGTCCACTAACTCCCTCTGCTAGGATA
TCAGCACTAAACATCGTGGGGGATCTCTTACGGAAAGTAGGGGCTTTAGA
ATCCAAATTAGCAGCTTGCAGGAATTTTGCAAAGGACCAAGCATCACGAA
AATCCTATATTTCAGGGAATGTTAACTGTGGGGTGCTGAATGGCAATGGC
ACAAAGTTCTCTCGATCAGGGCATACATCTTTCTTCGACAAAGGGGCAGT
AAACGGCTTTGACCCCGCTCCTCCTCCTCCTGGTCTGGGCTCCTCGCGTC
CATCGTCAGCGCCGGGTATGCTGCCTCTCAGTGTGTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMDGEDIPDFSSLKEETAYW
KELSLKYKQSFQEARDELVEFQEGSRELEAELEAQLVQAEQRNRDLQADN
QRLKYEVEALKEKLEHQYAQSYKQVSVLEDDLSQTRAIKEQLHKYVRELE
QANDDLERAKRATIVSLEDFEQRLNQAIERNAFLESELDEKESLLVSVQR
LKDEARDLRQELAVRERQQEVTRKSAPSSPTLDCEKMDSAVQASLSLPAT
PVGKGTENTFPSPKAIPNGFGTSPLTPSARISALNIVGDLLRKVGALESK
LAACRNFAKDQASRKSYISGNVNCGVLNGNGTKFSRSGHTSFFDKGAVNG
FDPAPPPPGLGSSRPSSAPGMLPLSV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11524
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 65196 Daltons