cDNA Clone Alpha actinin 3

• Plasmid: pCMV5-HA alpha actinin 3
• Parent Plasmid: pCMV5 HA1
• Expressed: HA alpha actinin 3
• Genbank: NM_001104.1 GI:4557240
• Tag: HA
• Species: Human

Sequence of Insert

gaattcatgatgatggttatgcagcccgagggtctgggggccggggaggg
gcgctttgcgggcggcggcgggggcggcgagtacatggaacaggaggagg
actgggaccgcgacctgctgctggacccggcctgggagaagcagcagcgg
aaaaccttcactgcctggtgcaactcacacctgcgcaaggcaggcaccca
gatcgagaacatcgaggaagatttccgcaatggcctcaaactcatgctgc
tcctggaggtcatttcaggtgagaggctgcctaggccagataaaggcaag
atgcgcttccacaaaatcgccaacgttaacaaggccctggacttcattgc
cagcaagggggttaaactggtgtccattggtgctgaagagattgttgacg
ggaacctgaagatgaccctgggcatgatctggaccatcatccttcgcttc
gccatccaggacatctctgtggaagaaacctcagccaaggaaggcttgct
tctgtggtgccagaggaagacagcaccgtaccgcaacgtcaacgtgcaga
acttccacaccagctggaaggatggcctggccctctgtgccctcatccac
cgacaccgccctgacctcatcgactacgccaaactgcgaaaggatgaccc
catcggaaacctgaacactgcctttgaggtggcagagaaatacctggaca
tccccaagatgttggatgcagaagacattgtgaacaccccaaagccggat
gagaaggccatcatgacctatgtgtcctgcttctaccatgcctttgccgg
ggctgagcaggcagagacagctgccaacaggatctgcaaggtgctggcag
tgaaccaggaaaacgagaagctgatggaggagtatgagaagcttgccagt
gagctgctggagtggatccgccgcactgtcccatggctggagaaccgtgt
gggtgagcccagcatgagtgccatgcagcgcaaactagaggactttcggg
actaccggcgtctgcacaagccgccccgcattcaggaaaagtgccagctg
gagatcaacttcaacacactgcagaccaagttgcggctcagccaccggcc
tgccttcatgccctccgagggcaagctggtctcggacatcgccaacgcct
ggcgggggctggagcaggtggaaaagggctatgaggactggctgctctcg
gagatccggcgcctgcagcgactccagcacctggctgagaagttccggca
gaaggcctccctgcacgaagcctggacccggggaaaggaggagatgctga
gccagcgcgactacgattcggctttgctacaggaggtgcgggcgttgctg
cggcgccacgaggcctttgagagcgacctggcggcgcaccaggaccgcgt
ggagcacattgccgcgctggcccaggagctcaatgagctggactaccacg
aggcagcctcagtgaatagccgctgccaggccatctgcgatcagtgggac
aacctgggcaccctgacccagaagaggcgggatgcgctagagcggatgga
gaagctcctggagaccattgaccagctgcaactggagtttgcccggcggg
ccgcgcccttcaacaactggctggatggtgccgtggaggacctgcaggac
gtgtggctggtacactctgtggaggagacccagagcctgctgacagcgca
cgatcagttcaaggcaacactgcccgaggctgaccgagagcgaggtgcca
tcatgggcatccagggtgagatccagaagatctgccagacgtatgggctg
cggccctgctccaccaatccctacatcaccctcagcccgcaggacatcaa
caccaagtgggatatggtccgaaagctggtgcccagccgtgaccagacac
tgcaggaggagctggcacggcagcaggtaaacgagaggctccggcgacag
tttgcggcccaggccaatgccattggaccctggatccaggcgaaggtgga
ggaagtggggcggctggcagcagggctagctggctctctggaggagcaga
tggctgggctacggcagcaggagcagaacattatcaactacaagactaac
attgaccggctggagggtgaccaccagctgctgcaggagagcctggtgtt
cgacaataagcacaccgtctacagcatggagcacatccgcgtgggctggg
agcagctgctcacctccattgcccgcaccatcaatgaagtggagaaccag
gtactgacccgagacgccaagggactgagccaggagcagctcaacgagtt
ccgagcatccttcaaccactttgacaggaagcggaatgggatgatggagc
ctgatgacttccgagcttgcctcatctccatgggctatgacctgggggaa
gtggagtttgctcgcatcatgaccatggtggaccccaacgcagctggggt
ggtgaccttccaggccttcatagacttcatgacccgagagacagccgaga
ctgacacgactgagcaagttgtagcttccttcaagatcttggcaggagac
aagaactacatcacccccgaggagctgcggcgcgagctccctgccaagca
ggccgagtactgcatccgccgtatggtgccctacaagggatccggggccc
cggctggagccctggactacgtggccttctccagtgccctctatggggag
agcgacctttgagcggccgc

Amino Acid Sequence

MYPYDVPDYAGSPEFMMMVMQPEGLGAGEGRFAGGGGGGEYMEQEEDWDR
DLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRNGLKLMLLLEV
ISGERLPRPDKGKMRFHKIANVNKALDFIASKGVKLVSIGAEEIVDGNLK
MTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNVQNFHT
SWKDGLALCALIHRHRPDLIDYAKLRKDDPIGNLNTAFEVAEKYLDIPKM
LDAEDIVNTPKPDEKAIMTYVSCFYHAFAGAEQAETAANRICKVLAVNQE
NEKLMEEYEKLASELLEWIRRTVPWLENRVGEPSMSAMQRKLEDFRDYRR
LHKPPRIQEKCQLEINFNTLQTKLRLSHRPAFMPSEGKLVSDIANAWRGL
EQVEKGYEDWLLSEIRRLQRLQHLAEKFRQKASLHEAWTRGKEEMLSQRD
YDSALLQEVRALLRRHEAFESDLAAHQDRVEHIAALAQELNELDYHEAAS
VNSRCQAICDQWDNLGTLTQKRRDALERMEKLLETIDQLQLEFARRAAPF
NNWLDGAVEDLQDVWLVHSVEETQSLLTAHDQFKATLPEADRERGAIMGI
QGEIQKICQTYGLRPCSTNPYITLSPQDINTKWDMVRKLVPSRDQTLQEE
LARQQVNERLRRQFAAQANAIGPWIQAKVEEVGRLAAGLAGSLEEQMAGL
RQQEQNIINYKTNIDRLEGDHQLLQESLVFDNKHTVYSMEHIRVGWEQLL
TSIARTINEVENQVLTRDAKGLSQEQLNEFRASFNHFDRKRNGMMEPDDF
RACLISMGYDLGEVEFARIMTMVDPNAAGVVTFQAFIDFMTRETAETDTT
EQVVASFKILAGDKNYITPEELRRELPAKQAEYCIRRMVPYKGSGAPAGA
LDYVAFSSALYGESDL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11519
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 105022 Daltons