cDNA Clone ESC4 (yeast)

• Plasmid: pRS415 RTT-FL-2Myc
• Parent Plasmid: pRS415
• Genbank: DQ332633
• Tag: Myc
• Species: Yeast

Sequence of Insert

GGATCCACCTCTCCAATAAGATATCCCAATAAGCTATTGAGAATTTCAAG
CGCGTGAAATTCAAATTTATCATCAGAGAACGATGTGCGGGAACTCCAGG
GCTTTTCTGTATTTTCTCATAGACATTATTTATCAGTAATTGCAGCTGTG
TAACAATAAAATTGACGCCACTTTCTTCGTCTTGTAGCTCCTCGTTAAGC
TGCGAAATTAACCTGCTCGTATTCACAGTAACACACTTAACCACAGAATG
TTCTTCGACATTAGAAATCTCTTGTATTTTTTGTACATCCCAAAAGAATT
CAGACATTTCTATTGAACCATTCCAGAAATGTTGTTCCAGAAGTTTATTC
TCTTGAACCTTTACACTATTTCGTATTTTCTCGGTTTTAGCCGCCCACTT
TTCACGCGTTAACAGGGTAAGCTATATCAGATGTGACTTTTCTTTTTCAC
TCATCTAATGTTGTTTTTCGCGTCACATTTTTTGCCAATAAATAAATACA
TCACAATGTTCTATATTGTATATTCAATGGAAGTCATTAGGTTATTGGGC
AAGATTTCAAGGAGGTAGCATACTGGTAAATATACGATCCATGAGCACAT
CACTTCTGTTTGAGCAGCTAAATTTTTTAATTTTAGTGGCAGCAGAGGCT
GAGCTTCCCATCGCTCACTCTACTAGAAAGCTTTTGATGGATAATTCATG
CAATAACTGTCAAATATACGAGCTTTATAACGAAAATTTGAAAGATGTCA
AGACGGATAAAGATTGGTTCATGAACAAATTCGGCCCGCAAACAGTACAT
TTTGTCATTAGTAATACAATCAACTTTCCTTTTTATAAGATAGTTTATTT
CGATCTTTTGATCCCGGTGGTATCACACACTTGGGTCCAGGATTCGGTCA
AGACGAAGAGACATTTGAGGACAAATATGTATTCACCTAACCCATTTCAT
TTGCTCCGAGACTGTCAAGTATATATTTCCAAAAGCTCGTTTAATAAGTG
TGAATACATATTATACTCTGATTTGCTTCATTTATTAGGAGGCACTTTAG
TCAATTACATCTCAAATAGAACGACGCATGTGATTGTTCAAAGTCCCCAG
GACCCGATCATAGCAACGGTCAGTAAATTGACATTTGGCTCTTTTTCCTC
TTCATCGACCAACAAGCACACCGAAAAGCCTTTGAGAGAGTGGAAATTTG
TCTATCCAATATGGATATTATATCATTTCAAAATGGCGAAGCCCTTGAAG
GGCGAGCTTGCTACTTTGTGTGAGCTTGATATGCAGGACACCAGTGAGGA
ACAGTTATTCGCAAAATGGGAAGAGGTTATTGGCGATAAGCAGACATCAT
CATCGCAGCTTACTCTTCATCCAAACAAAACTTTATTCAAAAATCACCAC
TTTGCTATCTCACCAGATTTAAACTTTTTCACACCTTTGTATTGGTTTTT
GAAAGGATTTATCGAGGATTTGGACGGCAAAGTAACTCCGTTGAGTTTTT
CTGACGATCTGAAGTCTGTTTACCAAGCTTTTCCTGATATTGACTGTTAT
ATCGGCCACTCTGCTAACTCACCGATACTAGAAAAAACCAAAAGCATTAA
ACCGGAAATTCATGTTGGGAACGTAAGTTGGTTGTTTTATATGTTTGCGC
TGCAAAAATTTACACCTGTATCGCAATGTAAACTTATTCACCAACCATTT
CATGCGAAATTATTTACTTCTAAGGAATTAACAGTCGCGTACACTAATTA
TTTCGGCTCCCAAAGGTTTTATATTCAGAGGTTAGTAGAAATATTAGGAG
GACTTAGCACACCTGAACTAACAAGAAAAAACACACACTTGATCACGAAG
AGTACAATTGGCAAAAAATTCAAAGTCGCAAAAAAATGGTCACTGGACCC
GCAAAATGCCATTATTGTGACAAATCATATGTGGTTAGAGCAATGTTATA
TGAATAATTCCAAGTTAAACCCCAAAGACAGTAGATTTCAAAATTTTAAA
TTGGATGATAACATGGGATGGAATATCGGCCAAATAGGAATGGATCATTC
ATCTCTCCCAACTCCAAAAAACCTATCGATGGTGACTTACGACACGCAAT
CCATATCAGAGAAGCCGCCTCCCACAAATGATCAAATGGATCAAATTAAC
GATAACACCAATGTATTATCAAAGAAAGATGGTACGCCCATTAGTAGTTT
TGAGAACTCAATTGATGAAAAGATTGACAAATTACAAAAAATATCAGGAG
AGGTGGCTGTAACCCATTCTGGAGATTTAGAACGGAGTTTTGTTTCCAGA
CCTTCAAGGGCAAGTTTTCCAGTAGTTGACAGTAAAAAATCTAATTTACA
AAAGAAAGACTCAAACTCAGATATTAGTATGGAAACAGAGGTTTTCTGTG
AAGGACATGAAAAAAGAGAAGAAAAGGAGTTTACGAAACCTATTACGGAG
TATGATGCACCTAAGAAACAAGAAATAAGGGAACAGTCACGCAAGAAGAA
TGACATAGATTATAAAAAAGAAGAGGAAGAAACGGAGCTGCAAGTTCAGC
TAGGACAACGAACTAAACGAGAGATCAAAACTTCGAAGAAAAATGAAAAG
GAGAAAGAAACCAATGAATGTCACATTGAAGTAGATCAAATGACGAATGA
AAAGCAAGGGGAGGAAAGTACAGGAAAACTGATTTCTACGGAAGACGTCA
CTTCTAAAAAAGATACAGATAAATTCTCGCATTTGTTTGAAGGATTATCT
GATAATGATGATCACATTAATGACGAAAAACCGGCAGTTAATTCCAAATA
TACCACACCCAAGACGTCTCAAAATATTACGAGTGGCGTTGATACTCCAA
CCACCGCGCAAACGCAAGTTTTTATGCCATCAAGTGGCAATTCAAGGTTA
GCAAAGACACAAGCTGCGAAGAGATTGCATACAGATATTGAATCATTAAA
TGAATTTCAGAAAAACTTTAAAAGGAAGAGAATTGATAGCGAGGAGATTT
CATTGTCCCAAGATGTTGAAAGGTCAAATAATAACAAAGAACTGGCTACT
AAAGCGGAAAAGATATTAGCTAGATTTAATGAACTACCAAATTATGACCT
AAAAGCGGTTTGCACCGGTTGTTTCCATGATGGATTTAATGAGGTTGACA
TCGAAATATTGAACCAACTAGGTATAAAAATTTTTGACAATATTAAAGAA
ACGGATAAATTGAATTGTATTTTTGCTCCAAAGATTTTGAGAACCGAGAA
ATTTTTAAAAAGCTTAAGTTTTGAACCGTTAAAATTCGCATTGAAACCTG
AATTTATTATTGATTTACTGAAACAAATTCATTCTAAAAAAGATAAACTT
TCTCAAATAAATATAAACTTGTTTGATTATGAGATAAATGGAATTAATGA
GAGTATAATATCAAAAACCAAACTACCTACTAAGGTGTTCGAAAGAGCGA
ATATTAGATGCATTAACTTAGTCAATGATATACCGGGTGGTGTAGATACA
ATAGGCTCTGTTCTAAAGGCACATGGTATCGAAAAAATCAACGTTTTACG
TAGTAAGAAATGCACATTTGAAGACATAATCCCAAATGACGTTTCTAAGC
AGGAAAACGGAGGCATTTTTAAATACGTACTTATCGTCACCAAAGCTTCA
CAGGTTAAAAAATTTACCAAACTGATTAATGATCGTGATAAAAACGAAAC
AATTTTGATTGTTGAATGGAATTGGTGTGTTGAATCAATTTTTCATCTCA
ATGTTGATTTTACATCAAAGAAAAATGTTTTGTATCAAAAGAAAAATAAC
GGTGAACAAAAGCTAATCTCCGAGGAAGACTTGAACGGTGAACAAAAATT
AATCTCAGAAGAAGACTTGTAGAAGGGCGAATTCCACAGTGGATATCAAG
CTTATCGATACCGTCGAC

Amino Acid Sequence

MSTSLLFEQLNFLILVAAEAELPIAHSTRKLLMDNSCNNCQIYELYNENL
KDVKTDKDWFMNKFGPQTVHFVISNTINFPFYKIVYFDLLIPVVSHTWVQ
DSVKTKRHLRTNMYSPNPFHLLRDCQVYISKSSFNKCEYILYSDLLHLLG
GTLVNYISNRTTHVIVQSPQDPIIATVSKLTFGSFSSSSTNKHTEKPLRE
WKFVYPIWILYHFKMAKPLKGELATLCELDMQDTSEEQLFAKWEEVIGDK
QTSSSQLTLHPNKTLFKNHHFAISPDLNFFTPLYWFLKGFIEDLDGKVTP
LSFSDDLKSVYQAFPDIDCYIGHSANSPILEKTKSIKPEIHVGNVSWLFY
MFALQKFTPVSQCKLIHQPFHAKLFTSKELTVAYTNYFGSQRFYIQRLVE
ILGGLSTPELTRKNTHLITKSTIGKKFKVAKKWSLDPQNAIIVTNHMWLE
QCYMNNSKLNPKDSRFQNFKLDDNMGWNIGQIGMDHSSLPTPKNLSMVTY
DTQSISEKPPPTNDQMDQINDNTNVLSKKDGTPISSFENSIDEKIDKLQK
ISGEVAVTHSGDLERSFVSRPSRASFPVVDSKKSNLQKKDSNSDISMETE
VFCEGHEKREEKEFTKPITEYDAPKKQEIREQSRKKNDIDYKKEEEETEL
QVQLGQRTKREIKTSKKNEKEKETNECHIEVDQMTNEKQGEESTGKLIST
EDVTSKKDTDKFSHLFEGLSDNDDHINDEKPAVNSKYTTPKTSQNITSGV
DTPTTAQTQVFMPSSGNSRLAKTQAAKRLHTDIESLNEFQKNFKRKRIDS
EEISLSQDVERSNNNKELATKAEKILARFNELPNYDLKAVCTGCFHDGFN
EVDIEILNQLGIKIFDNIKETDKLNCIFAPKILRTEKFLKSLSFEPLKFA
LKPEFIIDLLKQIHSKKDKLSQININLFDYEINGINESIISKTKLPTKVF
ERANIRCINLVNDIPGGVDTIGSVLKAHGIEKINVLRSKKCTFEDIIPND
VSKQENGGIFKYVLIVTKASQVKKFTKLINDRDKNETILIVEWNWCVESI
FHLNVDFTSKKNVLYQKKNNGEQKLISEEDLNGEQKLISEEDL*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11514
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence difference compared with DQ332633 CDS:; t3006g (silent)