cDNA Clone Ddb2

• Plasmid: pFastbacHTb Ddb2
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-Ddb2
• Genbank: NM_000107.1 GI:4557514
• Tag: 6His
• Species: Human

Sequence of Insert

ggatccatggctcccaagaaacgcccagaaacccagaagacctccgagat
tgtattacgccccaggaacaagaggagcaggagtcccctggagctggagc
ccgaggccaagaagctctgtgcgaagggctccggtcctagcagaagatgt
gactcagactgcctctgggtggggctggctggcccacagatcctgccacc
atgccgcagcatcgtcaggaccctccaccagcataagctgggcagagctt
cctggccatctgtccagcaggggctccagcagtcctttttgcacactctg
gattcttaccggatattacaaaaggctgccccctttgacaggagggctac
atccttggcgtggcacccaactcaccccagcaccgtggctgtgggttcca
aagggggagatatcatgctctggaattttggcatcaaggacaaacccacc
ttcatcaaagggattggagctggagggagcatcactgggctgaagtttaa
ccctctcaataccaaccagttttacgcctcctcaatggagggaacaacta
ggctgcaagactttaaaggcaacattctacgagtttttgccagctcagac
accatcaacatctggttttgtagcctggatgtgtctgctagtagccgaat
ggtggtcacaggagacaacgtggggaacgtgatcctgctgaacatggacg
gcaaagagctttggaatctcagaatgcacaaaaagaaagtgacgcatgtg
gccctgaacccatgctgtgattggttcctggccacagcctccgtagatca
aacagtgaaaatttgggacctgcgccaggttagagggaaagccagcttcc
tctactcgctgccgcacaggcatcctgtcaacgcagcttgtttcagtccc
gatggagcccggctcctgaccacggaccagaagagcgagatccgagttta
ctctgcttcccagtgggactgccccctgggcctgatcccgcaccctcacc
gtcacttccagcacctcacacctatcaaggcagcctggcatcctcgctac
aacctcattgttgtgggccgatacccagatcctaatttcaaaagttgtac
cccttatgaattgaggacgatcgacgtgttcgatggaaactcagggaaga
tgatgtgtcagctctatgacccagaatcttctggcatcagttcgcttaat
gaattcaatcccatgggggacacgctggcctctgcaatgggttaccacat
tctcatctggagccaggaggaagccaggacacggaagtgaggatcc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMAPKKRPETQKTSEIVLRPRNK
RSRSPLELEPEAKKLCAKGSGPSRRCDSDCLWVGLAGPQILPPCRSIVRT
LHQHKLGRASWPSVQQGLQQSFLHTLDSYRILQKAAPFDRRATSLAWHPT
HPSTVAVGSKGGDIMLWNFGIKDKPTFIKGIGAGGSITGLKFNPLNTNQF
YASSMEGTTRLQDFKGNILRVFASSDTINIWFCSLDVSASSRMVVTGDNV
GNVILLNMDGKELWNLRMHKKKVTHVALNPCCDWFLATASVDQTVKIWDL
RQVRGKASFLYSLPHRHPVNAACFSPDGARLLTTDQKSEIRVYSASQWDC
PLGLIPHPHRHFQHLTPIKAAWHPRYNLIVVGRYPDPNFKSCTPYELRTI
DVFDGNSGKMMCQLYDPESSGISSLNEFNPMGDTLASAMGYHILIWSQEE
ARTRK*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU11499
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; Sequence difference compared with NM_000107.1 CDS: ; c1017t (silent); ; Estimated MW of expressed protein : 51234 Daltons