cDNA Clone ERK5

• Synonyms: MAPK7
• Plasmid: pLZRSpBMN GFP-ERK5 1-740
• Parent Plasmid: pLZRS BMN GFP
• Expressed: GFP-ERK5 1-740
• Genbank: NM_002749
• Tag: GFP
• Species: Human

Sequence of Insert

AAGCTTGCCACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGT
GCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCG
TGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAG
TTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGAC
CACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGA
AGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAG
CGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGT
GAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCG
ACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTAC
AACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAA
GGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCG
CCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTG
CCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAA
CGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGA
TCACTCTCGGCATGGACGAGCTGTACAAGTCCGGACTCAGATCCATGGCC
GAGCCTCTGAAGGAGGAAGACGGCGAGGACGGCTCTGCGGAGCCCCCCGG
GCCCGTGAAGGCCGAACCCGCCCACACCGCTGCCTCTGTAGCGGCCAAGA
ACCTGGCCCTGCTTAAAGCCCGCTCCTTCGATGTGACCTTTGACGTGGGC
GACGAGTACGAGATCATCGAGACCATAGGCAACGGGGCCTATGGAGTGGT
GTCCTCCGCCCGCCGCCGCCTCACCGGCCAGCAGGTGGCCATCAAGAAGA
TCCCTAATGCTTTCGATGTGGTGACCAATGCCAAGCGGACCCTCAGGGAG
CTGAAGATCCTCAAGCACTTTAAACACGACAACATCATCGCCATCAAGGA
CATCCTGAGGCCCACCGTGCCCTATGGCGAATTCAAATCTGTCTACGTGG
TCCTGGACCTGATGGAAAGCGACCTGCACCAGATCATCCACTCCTCACAG
CCCCTCACACTGGAACACGTGCGCTACTTCCTGTACCAACTGCTGCGGGG
CCTGAAGTACATGCACTCGGCTCAGGTCATCCACCGTGACCTGAAGCCCT
CCAACCTATTGGTGAATGAGAACTGTGAGCTCAAGATTGGTGACTTTGGT
ATGGCTCGTGGCCTGTGCACCTCGCCCGCTGAACATCAGTACTTCATGAC
TGAGTATGTGGCCACGCGCTGGTACCGTGCGCCCGAGCTCATGCTCTCTT
TGCATGAGTATACACAGGCTATTGACCTCTGGTCTGTGGGCTGCATCTTT
GGTGAGATGCTGGCCCGGCGCCAGCTCTTCCCAGGCAAAAACTATGTACA
CCAGCTACAGCTCATCATGATGGTGCTGGGTACCCCATCACCAGCCGTGA
TTCAGGCTGTGGGGGCTGAGAGGGTGCGGGCCTATATCCAGAGCTTGCCA
CCACGCCAGCCTGTGCCCTGGGAGACAGTGTACCCAGGTGCCGACCGCCA
GGCCCTATCACTGCTGGGTCGCATGCTGCGTTTTGAGCCCAGCGCTCGCA
TCTCAGCAGCTGCTGCCCTTCGCCACCCTTTCCTGGCCAAGTACCATGAT
CCTGATGATGAGCCTGACTGTGCCCCGCCCTTTGACTTTGCCTTTGACCG
CGAAGCCCTCACTCGGGAGCGCATTAAGGAGGCCATTGTGGCTGAAATTG
AGGACTTCCATGCAAGGCGTGAGGGCATCCGCCAACAGATCCGCTTCCAG
CCTTCTCTACAGCCTGTGGCTAGTGAGCCTGGCTGTCCAGATGTTGAAAT
GCCCAGTCCCTGGGCTCCCAGTGGGGACTGTGCCATGGAGTCTCCACCAC
CAGCCCCGCCACCATGCCCCGGCCCTGCACCTGACACCATTGATCTGACC
CTGCAGCCACCTCCACCAGTCAGTGAGCCTGCCCCACCAAAGAAAGATGG
TGCCATCTCAGACAATACTAAGGCTGCCCTTAAAGCTGCCCTGCTCAAGT
CTTTGAGGAGCCGGCTCAGAGATGGCCCCAGCGCACCCCTGGAGGCTCCT
GAGCCTCGGAAGCCGGTGACAGCCCAGGAGCGCCAGCGGGAGCGGGAGGA
GAAGCGGCGGAGGCGGCAAGAACGAGCCAAGGAGCGGGAGAAACGGCGGC
AGGAGCGGGAGCGAAAGGAACGGGGGGCTGGGGCCTCTGGGGGCCCCTCC
ACTGACCCCTTGGCTGGACTAGTGCTCAGTGACAATGACAGAAGCCTGTT
GGAACGCTGGACTCGAATGGCCCGGCCCGCAGCCCCAGCCCTCACCTCTG
TGCCGGCCCCTGCCCCAGCGCCAACGCCAACCCCAACCCCAGTCCAACCT
ACCAGTCCTCCTCCTGGCCCTGTAGCCCAGCCCACTGGCCCGCAACCACA
ATCTGCGGGCTCTACCTCTGGCCCTGTACCCCAGCCTGCCTGCCCACCCC
CTGGCCCTGCACCCCACCCCACTGGCCCTCCTGGGCCCATCCCTGTCCCC
GCGCCACCCCAGATTGCCACCTCCACCAGCCTCCTGGCTGCCCAGTCACT
TGTGCCACCCCCTGGGCTGCCTGGCTCCAGCACCCCAGGAGTTTTGCCTT
ACTTCCCACCTGGCCTGCCGCCCCCAGACGCCGGGGGAGCCCCTCAGTCT
TCCATGTCAGAGTCACCCGACGTCAACCTTGTGACCCAGCAGCTATCTAA
GTCACAGGTGGAGGACCCCCTGCCCCCTGTGTTCTCAGGCACACCAAAGG
GCAGTGGGGCTGGCTAgGGTGTTGGCTTTGACCTGGAGGAATTCTTAAAC
CAGTCTTTCGACATGGGCGTGGCTGATGGGCCACAGGATGGCCAGGCAGA
TTCAGCCTCTCTCTCAGCCTCCCTGCTTGCTGACTGGCTCGAAGGCCATG
GCATGAACCCTGCCGATATTGAGTCCCTGCAGCGTGAGATCCAGATGGAC
TCCCCAATGCTGCTGGCTGACCTGCCTGACCTCCAGGACCCCTGAGCGGC
CGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSMAEPLK
EEDGEDGSAEPPGPVKAEPAHTAASVAAKNLALLKARSFDVTFDVGDEYE
IIETIGNGAYGVVSSARRRLTGQQVAIKKIPNAFDVVTNAKRTLRELKIL
KHFKHDNIIAIKDILRPTVPYGEFKSVYVVLDLMESDLHQIIHSSQPLTL
EHVRYFLYQLLRGLKYMHSAQVIHRDLKPSNLLVNENCELKIGDFGMARG
LCTSPAEHQYFMTEYVATRWYRAPELMLSLHEYTQAIDLWSVGCIFGEML
ARRQLFPGKNYVHQLQLIMMVLGTPSPAVIQAVGAERVRAYIQSLPPRQP
VPWETVYPGADRQALSLLGRMLRFEPSARISAAAALRHPFLAKYHDPDDE
PDCAPPFDFAFDREALTRERIKEAIVAEIEDFHARREGIRQQIRFQPSLQ
PVASEPGCPDVEMPSPWAPSGDCAMESPPPAPPPCPGPAPDTIDLTLQPP
PPVSEPAPPKKDGAISDNTKAALKAALLKSLRSRLRDGPSAPLEAPEPRK
PVTAQERQREREEKRRRRQERAKEREKRRQERERKERGAGASGGPSTDPL
AGLVLSDNDRSLLERWTRMARPAAPALTSVPAPAPAPTPTPTPVQPTSPP
PGPVAQPTGPQPQSAGSTSGPVPQPACPPPGPAPHPTGPPGPIPVPAPPQ
IATSTSLLAAQSLVPPPGLPGSSTPGVLPYFPPGLPPPDAGGAPQSSMSE
SPDVNLVTQQLSKSQVEDPLPPVFSGTPKGSGAG*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11470
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Retroviral expression vector. Ex Doreen Cantrell’s lab.; Nucleotide sequence difference compared with NM_002749 CDS:; t2127c (silent)