cDNA Clone ESC4 (yeast)

• Plasmid: pACT2.6 ESC4
• Parent Plasmid: pACT2.6
• Species: Yeast

Sequence of Insert

ggatccATGAGCACATCACTTCTGTTTGAGCAGCTAAATTTTTTAATTTTAGTGGCAGCAGAGGCTGAGCTTCCCATCGCTCACTCTACTAGAAAGCTTTTGATGGATAATTCATGCAATAACTGTCAAATATACGAGCTTTATAACGAAAATTTGAAAGATGTCAAGACGGATAAAGATTGGTTCATGAACAAATTCGGCCCGCAAACAGTACATTTTGTCATTAGTAATACAATCAACTTTCCTTTTTATAAGATAGTTTATTTCGATCTTTTGATCCCGGTGGTATCACACACTTGGGTCCAG
GATTCGGTCAAGACGAAGAGACATTTGAGGACAAATATGTATTCACCTAA
CCCATTTCATTTGCTCCGAGACTGTCAAGTATATATTTCCAAAAGCTCGT
TTAATAAGTGTGAATACATATTATACTCTGATTTGCTTCATTTATTAGGA
GGCACTTTAGTCAATTACATCTCAAATAGAACGACGCATGTGATTGTTCA
AAGTCCCCAGGACCCGATCATAGCAACGGTCAGTAAATTGACATTTGGCT
CTTTTTCCTCTTCATCGACCAACAAGCACACCGAAAAGCCTTTGAGAGAG
TGGAAATTTGTCTATCCAATATGGATATTATATCATTTCAAAATGGCGAA
GCCCTTGAAGGGCGAGCTTGCTACTTTGTGTGAGCTTGATATGCAGGACA
CCAGTGAGGAACAGTTATTCGCAAAATGGGAAGAGGTTATTGGCGATAAG
CAGACATCATCATCGCAGCTTACTCTTCATCCAAACAAAACTTTATTCAA
AAATCACCACTTTGCTATCTCACCAGATTTAAACTTTTTCACACCTTTGT
ATTGGTTTTTGAAAGGATTTATCGAGGATTTGGACGGCAAAGTAACTCCG
TTGAGTTTTTCTGACGATCTGAAGTCTGTTTACCAAGCTTTTCCTGATAT
TGACTGTTATATCGGCCACTCTGCTAACTCACCGATACTAGAAAAAACCA
AAAGCATTAAACCGGAAATTCATGTTGGGAACGTAAGTTGGTTGTTTTAT
ATGTTTGCGCTGCAAAAATTTACACCTGTATCGCAATGTAAACTTATTCA
CCAACCATTTCATGCGAAATTATTTACTTCTAAGGAATTAACAGTCGCGT
ACACTAATTATTTCGGCTCCCAAAGGTTTTATATTCAGAGGTTAGTAGAA
ATATTAGGAGGACTTAGCACACCTGAACTAACAAGAAAAAACACACACTT
GATCACGAAGAGTACAATTGGCAAAAAATTCAAAGTCGCAAAAAAATGGT
CACTGGACCCGCAAAATGCCATTATTGTGACAAATCATATGTGGTTAGAG
CAATGTTATATGAATAATTCCAAGTTAAACCCCAAAGACAGTAGATTTCA
AAATTTTAAATTGGATGATAACATGGGATGGAATATCGGCCAAATAGGAA
TGGATCATTCATCTCTCCCAACTCCAAAAAACCTATCGATGGTGACTTAC
GACACGCAATCCATATCAGAGAAGCCGCCTCCCACAAATGATCAAATGGA
TCAAATTAACGATAACACCAATGTATTATCAAAGAAAGATGGTACGCCCA
TTAGTAGTTTTGAGAACTCAATTGATGAAAAGATTGACAAATTACAAAAA
ATATCAGGAGAGGTGGCTGTAACCCATTCTGGAGATTTAGAACGGAGTTT
TGTTTCCAGACCTTCAAGGGCAAGTTTTCCAGTAGTTGACAGTAAAAAAT
CTAATTTACAAAAGAAAGACTCAAACTCAGATATTAGTATGGAAACAGAG
GTTTTCTGTGAAGGACATGAAAAAAGAGAAGAAAAGGAGTTTACGAAACC
TATTACGGAGTATGATGCACCTAAGAAACAAGAAATAAGGGAACAGTCAC
GCAAGAAGAATGACATAGATTATAAAAAAGAAGAGGAAGAAACGGAGCTG
CAAGTTCAGCTAGGACAACGAACTAAACGAGAGATCAAAACTTCGAAGAA
AAATGAAAAGGAGAAAGAAACCAATGAATGTCACATTGAAGTAGATCAAA
TGACGAATGAAAAGCAAGGGGAGGAAAGTACAGGAAAACTGATTTCTACG
GAAGACGTCACTTCTAAAAAAGATACAGATAAATTCTCGCATTTGTTTGA
AGGATTATCTGATAATGATGATCACATTAATGACGAAAAACCGGCAGTTA
ATTCCAAATATACCACACCCAAGACGTCTCAAAATATTACGAGTGGCGTT
GATACTCCAACCACCGCGCAAACGCAAGTTTTTATGCCATCAAGTGGCAA
TTCAAGGTTAGCAAAGACACAAGCTGCGAAGAGATTGCATACAGATATTG
AATCATTAAATGAATTTCAGAAAAACTTTAAAAGGAAGAGAATTGATAGC
GAGGAGATTTCATTGTCCCAAGATGTTGAAAGGTCAAATAATAACAAAGA
ACTGGCTACTAAAGCGGAAAAGATATTAGCTAGATTTAATGAACTACCAA
ATTATGACCTAAAAGCGGTTTGCACCGGTTGTTTCCATGATGGATTTAAT
GAGGTTGACATCGAAATATTGAACCAACTAGGTATAAAAATTTTTGACAA
TATTAAAGAAACGGATAAATTGAATTGTATTTTTGCTCCAAAGATTTTGA
GAACCGAGAAATTTTTAAAAAGCTTAAGTTTTGAACCGTTAAAATTCGCA
TTGAAACCTGAATTTATTATTGATTTACTGAAACAAATTCATTCTAAAAA
AGATAAACTTTCTCAAATAAATATAAACTTGTTTGATTATGAGATAAATG
GAATTAATGAGAGTATAATATCAAAAACCAAACTACCTACTAAGGTGTTC
GAAAGAGCGAATATTAGATGCATTAACTTAGTCAATGATATACCGGGTGG
TGTAGATACAATAGGCTCTGTTCTAAAGGCACATGGTATCGAAAAAATCA
ACGTTTTACGTAGTAAGAAATGCACATTTGAAGACATAATCCCAAATGAC
GTTTCTAAGCAGGAAAACGGAGGCATTTTTAAATACGTACTTATCGTCAC
CAAAGCTTCACAGGTTAAAAAATTTACCAAACTGATTAATGATCGTGATA
AAAACGAAACAATTTTGATTGTTGAATGGAATTGGTGTGTTGAATCAATT
TTTCATCTCAATGTTGATTTTACATCAAAGAAAAATGTTTTGTATCAAAA
GAAAAATAACTAActcgag

Amino Acid Sequence

MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSSNGPNLI
TTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTA
YNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEICMAYPYDVPDYAS
LGGHMAMGGSMSTSLLFEQLNFLILVAAEAELPIAHSTRKLLMDNSCNNC
QIYELYNENLKDVKTDKDWFMNKFGPQTVHFVISNTINFPFYKIVYFDLL
IPVVSHTWVQDSVKTKRHLRTNMYSPNPFHLLRDCQVYISKSSFNKCEYI
LYSDLLHLLGGTLVNYISNRTTHVIVQSPQDPIIATVSKLTFGSFSSSST
NKHTEKPLREWKFVYPIWILYHFKMAKPLKGELATLCELDMQDTSEEQLF
AKWEEVIGDKQTSSSQLTLHPNKTLFKNHHFAISPDLNFFTPLYWFLKGF
IEDLDGKVTPLSFSDDLKSVYQAFPDIDCYIGHSANSPILEKTKSIKPEI
HVGNVSWLFYMFALQKFTPVSQCKLIHQPFHAKLFTSKELTVAYTNYFGS
QRFYIQRLVEILGGLSTPELTRKNTHLITKSTIGKKFKVAKKWSLDPQNA
IIVTNHMWLEQCYMNNSKLNPKDSRFQNFKLDDNMGWNIGQIGMDHSSLP
TPKNLSMVTYDTQSISEKPPPTNDQMDQINDNTNVLSKKDGTPISSFENS
IDEKIDKLQKISGEVAVTHSGDLERSFVSRPSRASFPVVDSKKSNLQKKD
SNSDISMETEVFCEGHEKREEKEFTKPITEYDAPKKQEIREQSRKKNDID
YKKEEEETELQVQLGQRTKREIKTSKKNEKEKETNECHIEVDQMTNEKQG
EESTGKLISTEDVTSKKDTDKFSHLFEGLSDNDDHINDEKPAVNSKYTTP
KTSQNITSGVDTPTTAQTQVFMPSSGNSRLAKTQAAKRLHTDIESLNEFQ
KNFKRKRIDSEEISLSQDVERSNNNKELATKAEKILARFNELPNYDLKAV
CTGCFHDGFNEVDIEILNQLGIKIFDNIKETDKLNCIFAPKILRTEKFLK
SLSFEPLKFALKPEFIIDLLKQIHSKKDKLSQININLFDYEINGINESII
SKTKLPTKVFERANIRCINLVNDIPGGVDTIGSVLKAHGIEKINVLRSKK
CTFEDIIPNDVSKQENGGIFKYVLIVTKASQVKKFTKLINDRDKNETILI
VEWNWCVESIFHLNVDFTSKKNVLYQKKNN*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11444
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence differences in ESC4 compared with reference CDS:; t3006g (silent in S1002)