cDNA Clone KIF1

• Plasmid: pCMV5-HA KIF1
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-KIF1
• Genbank: NM_006612.4 GI:142386707
• Tag: HA
• Species: Human

Sequence of Insert

ggatccatggctggtgcctcggtgaaagtggcagtgagggttcggccctttaacgcccgtgagaccagccaggatgccaagtgtgtggtcagcatgcagggcaacaccacctccatcatcaatcctaaacagagcaaggatgcccccaaaagcttcacctttgactactcctactggtcacacacttcgacggaggacccccagtttgcatctcagcagcaagtgtatcgggacattggagaagagatgctgctccacgcctttgaaggctacaacgtgtgcatctttgcctatgggcagaccggg
gctgggaaatcctataccatgatggggcgacaggagccagggcagcaggg
catcgtgccccagctctgtgaggacctcttctctcgcgttagtgagaacc
agagtgctcagctatcctactctgtggaggtgagctatatggagatctac
tgtgagcgggtacgagacctcttgaaccccaagagtcggggttctctgcg
ggtccgggagcaccccatcctgggcccgtacgtgcaggacctgtccaaat
tggctgtgacctcctacgcagacattgctgacctcatggactgtggaaat
aaagcacggactgtggctgccaccaacatgaatgagaccagcagccgttc
ccatgccgtctttaccatcgtcttcacacagcgctgccatgaccagctca
cggggctggactcggagaaggtcagtaagatcagtttggtggaccttgct
gggagtgagcgagccgactcctcaggggcccggggcatgcgcctgaagga
aggagccaacatcaataagtccctgactacactagggaaagtgatctcgg
cccttgcagatatgcaatcaaagaagcgaaagtcggattttatcccctac
agggactctgtgctcacctggctgctcaaggaaaatttgggggggaactc
acgcacagccatgattgcagccctgagccctgctgacatcaattacgagg
agactctcagcaccctcaggtatgctgaccgcaccaagcaaatccgctgc
aatgccatcatcaacgaggaccctaatgcccggctgattagagagctgca
ggaggaagtagcccggctgcgggaactgctgatggctcagggactgtcag
cctctgctctggaaggcctgaagacggaagaagggagtgtcagaggcgcc
ctgccagctgtgtcatctcccccagctccagtttcaccctcatcacccac
cacacataatggggagctggagccgtcattctcccccaacacggagtccc
agattgggcctgaggaagccatggagaggctgcaggagacagagaagatt
atagctgagctgaacgagacatgggaggagaagctacgcaagacagaagc
cctgaggatggagagagaagcattgctggctgagatgggggtggccgtcc
gggaggatgggggaactgtgggcgtcttctctccaaagaagactccccac
ctggtgaacctgaacgaagaccctctgatgtctgagtgtctgctctacca
catcaaagatggcgtcaccagggtcggccaagtagatatggacatcaagc
tgaccggacagttcattcgggagcaacactgtctgttccggagcatcccc
cagccagatggagaagtggtggtcactctggagccttgtgaaggagctga
gacatatgtgaatgggaagcttgtgacggagccgctggtgctgaagtcag
ggaataggattgtgatgggcaagaaccacgttttccgcttcaaccacccg
gagcaggcaaggctggaacgggaacgaggggtccccccacccccaggacc
gccctctgagccagtcgactggaactttgcccagaaggaactgctggagc
agcaaggcatcgacataaagctggaaatggagaagaggctgcaggatctg
gagaatcagtaccggaaagaaaaggaagaagccgatcttctgctggagca
gcagcgactgtatgcagactcggacagcggggatgactctgacaagcgct
cttgtgaagagagctggaggctcatctcctccttgcgggagcagctgccg
cccaccacggtccagaccattgtcaaacgctgtggtctgcccagcagtgg
caagcgcagggcccctcgcagggtttatcagatcccccagcgacgcaggc
tgcagggcaaagacccccgctgggccaccatggctgacctgaagatgcag
gcggtgaaggagatctgctacgaggtggccctggctgacttccgccacgg
gcgggctgagattgaggccctggccgccctcaagatgcgggagctgtgtc
gcacctatggcaagccagacggccccggagacgcctggagggctgtggcc
cgggatgtctgggacactgtaggcgaggaggaaggaggtggagctggcag
tggtggtggcagtgaggagggagcccgaggggcggaggtggaggacctcc
gggcccacatcgacaagctgacggggattctgcaggaggtgaagctgcag
aacagcagcaaggaccgggagctgcaggccctgcgggaccgcatgctccg
catggagagggtcatccccctggcccaggatcatgaggatgagaatgaag
aaggtggtgaggtcccctgggccccgcctgaaggatcagaggcagcagag
gaggcagcccccagtgaccgcatgccgtcagcccggcccccctcgccacc
actgtcaagctgggagcgggtgtcacggctcatggaggaggaccctgcct
tccgtcgtggtcgtcttcgctggctcaagcaggagcagctacggctgcag
ggactgcagggctctgggggccggggcggggggctgcgcaggcccccagc
ccgctttgtgccccctcacgactgcaagctacgcttccccttcaagagca
acccccagcaccgggagtcttggccagggatggggagcggggaggctcca
actccgctccaaccccctgaggaggtcactccccatccagccacccctgc
ccgccggcctccgagtccccgaaggtcccaccatccccgcaggaactccc
tggatggagggggccgatcccggggagcgggttctgcacagcctgaaccc
cagcacttccagcccaaaaagcacaactcttatccccagccaccccaacc
ctacccagcccagcggcccccagggccccgctaccccccatacactactc
ccccacgaatgagacggcagcgttctgcccctgacctcaaggagagtggg
gcagctgtgtgagcggccgc

Amino Acid Sequence

MYPYDVPDYAGSMAGASVKVAVRVRPFNARETSQDAKCVVSMQGNTTSII
NPKQSKDAPKSFTFDYSYWSHTSTEDPQFASQQQVYRDIGEEMLLHAFEG
YNVCIFAYGQTGAGKSYTMMGRQEPGQQGIVPQLCEDLFSRVSENQSAQL
SYSVEVSYMEIYCERVRDLLNPKSRGSLRVREHPILGPYVQDLSKLAVTS
YADIADLMDCGNKARTVAATNMNETSSRSHAVFTIVFTQRCHDQLTGLDS
EKVSKISLVDLAGSERADSSGARGMRLKEGANINKSLTTLGKVISALADM
QSKKRKSDFIPYRDSVLTWLLKENLGGNSRTAMIAALSPADINYEETLST
LRYADRTKQIRCNAIINEDPNARLIRELQEEVARLRELLMAQGLSASALE
GLKTEEGSVRGALPAVSSPPAPVSPSSPTTHNGELEPSFSPNTESQIGPE
EAMERLQETEKIIAELNETWEEKLRKTEALRMEREALLAEMGVAVREDGG
TVGVFSPKKTPHLVNLNEDPLMSECLLYHIKDGVTRVGQVDMDIKLTGQF
IREQHCLFRSIPQPDGEVVVTLEPCEGAETYVNGKLVTEPLVLKSGNRIV
MGKNHVFRFNHPEQARLERERGVPPPPGPPSEPVDWNFAQKELLEQQGID
IKLEMEKRLQDLENQYRKEKEEADLLLEQQRLYADSDSGDDSDKRSCEES
WRLISSLREQLPPTTVQTIVKRCGLPSSGKRRAPRRVYQIPQRRRLQGKD
PRWATMADLKMQAVKEICYEVALADFRHGRAEIEALAALKMRELCRTYGK
PDGPGDAWRAVARDVWDTVGEEEGGGAGSGGGSEEGARGAEVEDLRAHID
KLTGILQEVKLQNSSKDRELQALRDRMLRMERVIPLAQDHEDENEEGGEV
PWAPPEGSEAAEEAAPSDRMPSARPPSPPLSSWERVSRLMEEDPAFRRGR
LRWLKQEQLRLQGLQGSGGRGGGLRRPPARFVPPHDCKLRFPFKSNPQHR
ESWPGMGSGEAPTPLQPPEEVTPHPATPARRPPSPRRSHHPRRNSLDGGG
RSRGAGSAQPEPQHFQPKKHNSYPQPPQPYPAQRPPGPRYPPYTTPPRMR
RQRSAPDLKESGAAV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11441
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 124301 Daltons