cDNA Clone Mms1 (yeast)

• Plasmid: pRS415 Pro-15HA-Mms1
• Parent Plasmid: pRS415
• Genbank: NC_001148
• Tag: 15HA
• Species: Yeast

Sequence of Insert

CTGCAGCCCGGGGGATCCACTAGTTCTAGAGCGGCCGCTCGAGTTTTATGATAATCCCGCTTATTGGTCTACTGATCTTCTCTAACTCTGTAGTTATATCTATCTCTTCTGCTTCTATTTCTGCTTTTCTTTAGCGATGCTGATTTCCGCAAAAAAATTTTCATTCCGTGGTGCGTGATCTGATGAGAAATAAAGCCAAAAAGAAAAAAAGACAAGAAGACAAAAAGGTTGGGCGGCTAAGTAGCGCCAGGATATAAAAAGCAACATAGATTTCAGTACCCTATTATCCTGCCAAAATTTACTTTAAGAAGTCTCTATGAATATACAGGCTAAATTTGGAAACATGAGAAAGGAAAGATGAAGATACAAAGATTGCAAGCAGTCAAGAAGATTTAGGAAAAAAGGAAAAAGCAAAAAATATCGATTTTTATGACTTACAAACTAAATTGATATGTTCGAAATAATTTTGAGAAGCTGAGCTCAATCTGGTACTAATCACTTCAAGTTAGATGTGCAAAGAAAGAGAATAGCATAAATTCAAAACCAAAGGAAGTGCGAGGCATTACCCGCATATTATCGGGAAACAGAAGCCATGTTAGAGTGATTTCCAACAAATGGCCCTTTGCCAGAGATGGGCTCTGCAAAAGAATTGGAAGAAGAGAAGAAATCGCTTCACATTCCGTTTCGTTTCTGGGTCTAATTAAAGTAGTACGGTGTCAATTAGCCATGGGCTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATTACGCTGCTCAGTGCGGCCGCATCTTTTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATTACGCTGCTATGGGCTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATTACGCTGCTATGGGCTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATTACGCTGCTATGGGCTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGAT
TACGCTGCCATGGTAGGTTTGCGAACTCATGGTTTAGACAGGTATGAACATTATATTCGTCGCCCGTCGGATTTTGGCAAACTTGAGCTGCAAGATTGGTTGAATCATAAGTCATTCCGAGTTTCCCCCAATTTATTAATTGATTCAAGCACCACACGTGAGTGGAATGAACCTGAGCTTTTTTATCAAAATACCGAGGATGAAACTTGGGTGAGACCTTGTGTAGGGCCAAAGCTAGAACCATCAATGATGATGCTCAGATATCACGATTCGAATATTGGTCAAATGCCTCAATTTTGTTATCCAATCTCAAGTCCGATAAATTTTAAACCAGTATTAAAATACATTTTGCAAGAACGTTCTGAACTGTCAGACGGCTTCCCTCAAAAGTATAACACACTAATAGGTAGTCTTTTTGACATTGATAAAAACCCAGAAACCTTAGATGATTCAGATATAGAAGCATTGGATGACATAGAAATGAGCAGTGACAGCGGTAATGTTAAAGAACCAAAAATTGAATTGCAGGCGCTGGAAGAAATCCAACAAAAGCATTTCAGTTTAATAGTATCCAACAATGGAATCTTTCAAACAGGTAGCACTTCAATAACATACATACAGTCTGGCATATCTGGCAGCATAGCTATAAAACCCAACAACGTTGCAATTTTAATATTACTCACTCAACCAAGTGGTCACTTATTGTCTATTTTACCGCTTGATGACGGTAAAGAGACATATTTGCTACAATATTGGAACCTGGGACAAAAAGGTCAGTGGAACATAATCAAGCACCAAAACGAGAAGCAGTTTGTTCTTATACATAAGGAACTAGGCATTTGCAAATTTTTTGAATTCCATTTACCATTTACTTTTCAATTAGTAAACAATTTAACATTGACCGATTCCGTGATTATGAACGGATCCTTTTTCCCAACAAATTACACTGATTTAGATCCCTATTTCATTATATTTATAACAGCCATAAGATATGAAAGGATAGTCTACTTTGTCATAGAATGGAACAACAACGAAATAAAGAAAAAAGAGGTATATCAATTGACAGTATTTGATGGTGAGAAGACTAATATGACAATACCCATTGGACTAAATGCATGTTTAGTCGAAACACCCCTAAAGTTCTCTTTAGTTTCTGCAAATCAAATTATGTCAGGAGAGACTGAATTCCACTCATTCCAATTGAAGGCTCTCAAGGGAATCAAGTCATTTTTTCCAGCTCCTTTATTGTTATTAAAATTACAAGAACTACACCCACATACATTTAAAAAATTCCAATATTGTACCATAATATCCTCCTCG
ACAGGAAATATTTGTTTTTGCGTCACCGAACGATCGACAATAGTAAATGG
TAATTTAAAATTTTACGAGCTAACTAGGTTCAAAGGATTGAAATCCATTT
CACCACTACCTTCAAATCCGATAAATTTAGACTCCAGATCCTCAAGTTAT
GTACTGGTGGTCATAAGTTTTAGTAGAACATTAGAGCTAACATTATCTCT
AGAAGATTTGAGATGTTTAGATAAAAAAGACGTTATTAAGCCTTTGAAAA
ATATCACCTTCAAGCACACAATTGATAGTTCCACAGAGGAGAACTCTCAA
ATTTTAGCATTTACGTCTTCTAAATTTTATAACACACACACAGGCTCTAA
CATCAATGACACGAGAAATTCTCAAGTTTGGCTTACCTCACCAAATGCAA
TAACTCAACCTTGCATTGATTATAAACTCAGGAAAACTCATCAACTTATC
CACTTAAAGCAATTTCAAATTTTTAGACATCTTAGGATATGGAAATGTAA
GAACCTTGATATTGCTCTGTTACAGAGACTTGGAATAAACCAGTCAAATA
CCGAAAGTTCGTTAATTTTTGCGACCGACGCTGTTTCTAACAACAGAATA
TTTTTATTAGATTTAACTATGACAACGACAATCGATAATGATGATCCCGT
TCAAGGACTGATAAATATAGAAGATTTACTATGTGATACTGAAAACGAAA
CTATCCTTCTAAATTTTACGAAAAATAACTTGATTCAAGTAACTAGGGAT
ACGATATACATCGATCCCATTGGTGGGGACAAAGAGTTGCGTAAGATTTC
TCCAGGTTGGGAATTCGAAAACGTTACATACAATGATGGTATTTTAATAG
TTTGGAATGCTGGGCTTGGCTGTGTCTCTTATATTGAAAATATAGACGCT
GTTGATGAGTCTGGCGCCTTAGTTTCAAACTTAAGCAGCAGCAAAGGCAT
GAGCAAGTTCTTCAAGCAGTTAGGAACTGTCACGAGTGTCAATTTTCAAA
TCAAAGAGTCTACAGATGATCCAACCAAGTATGACATTTGGATCCTTTTA
CCAGACTGTGTCATTCGTACACCCTTTTCTGACTGGATTAGTGATTCACT
TGATTTTTCTGATGTGTATATTTTGAGTGTTCAGCAGGCGTTAATAAACG
GCCCTTATTTTTGCTCTCTCGATTATGAATCATATTTTGAGGTGCACACT
TTACAGAACAACTGCTTCAAAAAAGGATCCAGATGTACAAGCAGAGTTAA
TTTTCAAGGGAAAGATATTAAATTTAGAAGCTTTGGTGTGAATCAATGTT
TGGCATTTAGTGCATTTGAAATTTTTGTCATCAATTTAACGCCAATTCAC
GACAGTAGAGAATTGGATTTTTACAAGCTAAAATTACCGCACCTAGGTAA
TAACAATTCAATTCTTGAAGTCTGTCCGGACATAGAAAACAACCAATTAT
TCATACTCTACTCTGATGGCTTAAGAATCCTCGAACTATCATACCTAACA
TCAAATAATGGAAATTTCTTATTAAAATCTACAAGAAGCAAAAATAAGAA
ATTTTTATATCTAGACAAAATAAATCGAATGCTGGTATTGAATCAGGACT
TGAGGGAATGGGAGTGTATTAGACTATCAGATGGTAAGGCAGTTGGTTTA
GATTCTCAACTTCTTAAGGATGATTCTGAAGAAATCCTAGAAATAAAGGA
ATTACCAATAGCAACAGAGGACAATCCTTTAGAAAAGAAAACTGTATTAT
TGATCTCTTTTACTAGCTCATTAAAACTAGTTTTATTAACTGCTGCAAAA
AACAAAATTTCCAATCAAATAATAGATTCGTATAAACTTGACAATTCAAG
ACTCCTCAATCATTTGGTCATTACTCCCAGAGGTGAAATATTCTTTCTGG
ATTATAAAGTTATGGGCACCGATAACGAAATGTCCTTTAACAAGTTGAAA
GTCACAAAACATTGCATTGACCAGGAGGAGAGAAATAATACGACTTTGCG
GCTCACTTTGGAAACCCGGTTTACATTTAAGAGTTGGAGTACAGTTAAGA
CGTTTACTGTAGTAGGCGATAATATCATTGCTACCACAAATATGGGCGAA
AAGCTCTACTTGATTAAGGATTTCTCTTCATCATCTGACGAATCGAGAAG
GGTGTATCCCTTGGAAATGTATCCTGATTCAAAAGTTCAAAAGATAATAC
CATTAAATGAATGCTGCTTTGTTGTTGCCGCTTACTGTGGAAATAGGAAC
GATTTAGATTCAAGATTAATTTTTTACTCTTTACCCACCATCAAAGTTGG
GCTTAATAACGAAACAGGCAGCCTACCAGATGAATATGGGAATGGGAGAG
TCGACGACATATTCGAGGTTGACTTTCCTGAAGGATTTCAATTTGGCACT
ATGGCTTTGTATGATGTTTTACATGGTGAGAGGCACGTAAATCGTTACAG
CGAAGGCATACGGTCGGAGAATGACGAAGCAGAGGTTGCCCTAAGGCAGC
GTAGAAATTTACTACTCTTTTGGCGAAACCACTCTTCTACACCAAAACCT
TCACTACGCCGAGCCGCCACTATAGTATATGAGGATCATGTATCATCCCG
TTATTTTGAGGATATAAGTTCTATATTAGGAAGCACTGCAATGAGAACTA
AAAGACTATCTCCCTATAATGCGGTAGCATTGGACAAGCCTATTCAAGAT
ATTAGTTACGATCCCGCAGTACAAACTTTATATGTGCTAATGGCAGATCA
AACAATTCACAAATTCGGCAAGGACAGGTTGCCTTGCCAGGACGAATACG
AACCAAGATGGAATTCTGGCTATTTGGTTTCAAGAAGGTCAATAGTTAAA
TCTGACCTCATCTGTGAGGTTGGGTTATGGAACCTTAGCGATAACTGCAA
GAACACAGTATAACCGCGG

Amino Acid Sequence

MGYPYDVPDYAGYPYDVPDYAGSYPYDVPDYAAQCGRIFYPYDVPDYAGY
PYDVPDYAGSYPYDVPDYAAMGYPYDVPDYAGYPYDVPDYAGSYPYDVPD
YAAMGYPYDVPDYAGYPYDVPDYAGSYPYDVPDYAAMGYPYDVPDYAGYP
YDVPDYAGSYPYDVPDYAAMVGLRTHGLDRYEHYIRRPSDFGKLELQDWL
NHKSFRVSPNLLIDSSTTREWNEPELFYQNTEDETWVRPCVGPKLEPSMM
MLRYHDSNIGQMPQFCYPISSPINFKPVLKYILQERSELSDGFPQKYNTL
IGSLFDIDKNPETLDDSDIEALDDIEMSSDSGNVKEPKIELQALEEIQQK
HFSLIVSNNGIFQTGSTSITYIQSGISGSIAIKPNNVAILILLTQPSGHL
LSILPLDDGKETYLLQYWNLGQKGQWNIIKHQNEKQFVLIHKELGICKFF
EFHLPFTFQLVNNLTLTDSVIMNGSFFPTNYTDLDPYFIIFITAIRYERI
VYFVIEWNNNEIKKKEVYQLTVFDGEKTNMTIPIGLNACLVETPLKFSLV
SANQIMSGETEFHSFQLKALKGIKSFFPAPLLLLKLQELHPHTFKKFQYC
TIISSSTGNICFCVTERSTIVNGNLKFYELTRFKGLKSISPLPSNPINLD
SRSSSYVLVVISFSRTLELTLSLEDLRCLDKKDVIKPLKNITFKHTIDSS
TEENSQILAFTSSKFYNTHTGSNINDTRNSQVWLTSPNAITQPCIDYKLR
KTHQLIHLKQFQIFRHLRIWKCKNLDIALLQRLGINQSNTESSLIFATDA
VSNNRIFLLDLTMTTTIDNDDPVQGLINIEDLLCDTENETILLNFTKNNL
IQVTRDTIYIDPIGGDKELRKISPGWEFENVTYNDGILIVWNAGLGCVSY
IENIDAVDESGALVSNLSSSKGMSKFFKQLGTVTSVNFQIKESTDDPTKY
DIWILLPDCVIRTPFSDWISDSLDFSDVYILSVQQALINGPYFCSLDYES
YFEVHTLQNNCFKKGSRCTSRVNFQGKDIKFRSFGVNQCLAFSAFEIFVI
NLTPIHDSRELDFYKLKLPHLGNNNSILEVCPDIENNQLFILYSDGLRIL
ELSYLTSNNGNFLLKSTRSKNKKFLYLDKINRMLVLNQDLREWECIRLSD
GKAVGLDSQLLKDDSEEILEIKELPIATEDNPLEKKTVLLISFTSSLKLV
LLTAAKNKISNQIIDSYKLDNSRLLNHLVITPRGEIFFLDYKVMGTDNEM
SFNKLKVTKHCIDQEERNNTTLRLTLETRFTFKSWSTVKTFTVVGDNIIA
TTNMGEKLYLIKDFSSSSDESRRVYPLEMYPDSKVQKIIPLNECCFVVAA
YCGNRNDLDSRLIFYSLPTIKVGLNNETGSLPDEYGNGRVDDIFEVDFPE
GFQFGTMALYDVLHGERHVNRYSEGIRSENDEAEVALRQRRNLLLFWRNH
SSTPKPSLRRAATIVYEDHVSSRYFEDISSILGSTAMRTKRLSPYNAVAL
DKPIQDISYDPAVQTLYVLMADQTIHKFGKDRLPCQDEYEPRWNSGYLVS
RRSIVKSDLICEVGLWNLSDNCKNTV*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11435
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast expression plasmid for 15HA-tagged Mms1.; L2V in Mms1 due to Nco1 site.; ; template pRS415 Pro-13Myc-Mms1 (DU11421 digested with Nco1 and gel purified to remove 13myc)