cDNA Clone slit-robo-GAP 3

• Plasmid: pGEX6P-1-srGAP3a
• Parent Plasmid: pGEX6P1
• Expressed: GST-srGAP3a
• Genbank: NM_014850
• Tag: GST
• Species: Human

Sequence of Insert

gaattcatgtcatctcaaactaagttcaagaaagacaaagagatcattgctgaatatgaagcccaaataaaagagatccgcacgcagctggtggagcagttcaaatgtctggagcagcaatcagagtcgcgactgcagctgcttcaagacctccaggagtttttccgccggaaagctgagattgagctcgagtactcccgcagcctggagaagctggctgagcgcttctcctccaaaatccgcagctcccgggagcaccagttcaagaaggaccagtacctcctctcgcctgtgaactgttggtat
ctggttctgcatcagacccggcgggagagccgagaccatgccaccctcaa
tgacatcttcatgaacaatgtcatcgtccgcctctcccagatcagtgagg
atgtcatcagactcttcaaaaagagcaaggagattggcctgcagatgcac
gaggagctcctgaaggtgaccaatgagctctacacagtcatgaaaaccta
ccacatgtaccatgcagagagcatcagtgcggaaagcaagctgaaggagg
ctgagaagcaggaggagaagcagttcaataagtcaggagacctcagcatg
aacctgctccggcacgaggaccggccccagcgccgcagctctgtgaagaa
gattgagaagatgaaggagaagaggcaggccaagtactctgagaacaagc
tgaaatgcacaaaggcccggaatgactacttgctcaatctggcagccacc
aacgcagctataagcaaatactacatccatgatgtctctgatctgatcga
ttgctgtgatttgggcttccatgccagcctggcccgcaccttccggacct
atctctcagctgaatacaacctggagacctctcgccacgaagggctggat
gtcattgagaatgcagtggacaacctggattcccgaagtgacaagcacac
agtcatggacatgtgcaatcaagtcttctgccctccactcaagttcgagt
tccagccccacatgggggatgaggtctgccaggtcagcgctcagcagccc
gtccagacagaactgctcatgcgttatcaccagctgcagtccagactggc
caccctcaagatagagaatgaggaggttaggaaaaccctggatgccacca
tgcagacattacaggacatgctgactgtggaggactttgatgtctccgat
gccttccaacacagtcgatcgacagagtccgtcaagtcggctgcctctga
gacctacatgagcaagatcaacattgccaagaggagagccaaccagcagg
aaacagaaatgttttattttacaaaatttaaagagtatgtgaatggcagt
aacctcatcaccaagctgcaggccaagcacgatttactcaagcagaccct
gggcgaaggggaaagagcagaatgcggcaccaccaggcccccctgtcttc
cccctaaaccacagaaaatgaggagacctaggcctctctcagtgtatagc
cataaactctttaacggcagtatggaagcattcattaaggattcaggaca
agctataccgcttgtagtcgagagctgcatccgttacatcaatttatatg
gactccagcagcaggggatcttcagagtgccaggatctcaggtggaagtc
aatgacatcaaaaattcctttgagagaggtgaagacccccttgtggacga
tcaaaatgaacgagatatcaattcagtcgctggtgttttaaaactgtatt
tccgaggactggaaaacccactctttcctaaggaaaggtttcaagatttg
atatctactattaaactggagaacccagccgagagggtgcaccagatcca
acaaatcctcgtcacccttccccgcgtggtcattgtggtcatgagatacc
tcttcgctttcctcaaccacctctcccagtatagcgacgagaacatgatg
gatccctacaacctggccatctgcttcgggcctaccctcatgcacatccc
tgatgggcaggaccctgtgtcctgccaggcacacatcaatgaagtcatca
aaaccatcatcatccatcatgaagccatcttccccagcccccgggagcta
gagggacctgtgtatgaaaaatgcatggctggaggggaagaatattgtga
cagcccacacagcgagccaggggccatcgatgaagttgaccatgacaatg
gcactgagcctcataccagcgatgaagaagtggagcagatcgaggctatt
gccaagtttgactacatggggcggtccccgcgtgagctatccttcaagaa
gggggcctcgctgctcctgtaccaccgcgcctcggaggactggtgggagg
gccggcacaacggcgtggatggactcatcccccatcagtacatagttgta
caggacatggatgatgccttctccgacagcctgagccagaaggctgacag
cgaggccagcagtgggccattgctggatgacaaggcctcttccaaaaacg
acctccagtcccccacggagcacatctcggattacggctttgggggggtg
atgggccgagtgcggttacgatctgatggagcagccatccccagacgccg
aagcgggggcgacacacacagcccgccccggggcctgggccccagcatag
acacaccaccccgggctgctgcctgccccagcagcccccacaaaatcccc
ctcacccgggggaggatcgagagccctgagaagcggaggatggcgacgtt
cgggagcgctggcagcatcaactaccctgacaagaaggcgctctccgaag
ggcactcgatgaggtcgacctgcggttccaccaggcacagcagcctaggg
gaccacaagtccctggaggccgaggccctggcagaagacatcgagaagac
catgagcacggctctgcacgagttgcgggaactcgagaggcagaacacgg
tcaagcaggcgccagatgtggtgctggacaccctggagcccctgaagaac
ccgccaggccccgtcagctcggagcccgccagtccccttcacaccatcgt
catccgcgaccccgatgccgccatgcgccgcagcagcagctcctccaccg
agatgatgaccaccttcaagccagccctgtccgcccgcctggctggcgcc
cagctccgcccgccccccatgcggcccgtgcggccggtggtccagcaccg
gtccagcagcagcagcagctcgggcgtgggcagcccggccgtgacgccca
ccgagaagatgttccccaacagctcagcggacaagtcgggcaccatgtga
gcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMSSQTKFKKDKEIIAE
YEAQIKEIRTQLVEQFKCLEQQSESRLQLLQDLQEFFRRKAEIELEYSRS
LEKLAERFSSKIRSSREHQFKKDQYLLSPVNCWYLVLHQTRRESRDHATL
NDIFMNNVIVRLSQISEDVIRLFKKSKEIGLQMHEELLKVTNELYTVMKT
YHMYHAESISAESKLKEAEKQEEKQFNKSGDLSMNLLRHEDRPQRRSSVK
KIEKMKEKRQAKYSENKLKCTKARNDYLLNLAATNAAISKYYIHDVSDLI
DCCDLGFHASLARTFRTYLSAEYNLETSRHEGLDVIENAVDNLDSRSDKH
TVMDMCNQVFCPPLKFEFQPHMGDEVCQVSAQQPVQTELLMRYHQLQSRL
ATLKIENEEVRKTLDATMQTLQDMLTVEDFDVSDAFQHSRSTESVKSAAS
ETYMSKINIAKRRANQQETEMFYFTKFKEYVNGSNLITKLQAKHDLLKQT
LGEGERAECGTTRPPCLPPKPQKMRRPRPLSVYSHKLFNGSMEAFIKDSG
QAIPLVVESCIRYINLYGLQQQGIFRVPGSQVEVNDIKNSFERGEDPLVD
DQNERDINSVAGVLKLYFRGLENPLFPKERFQDLISTIKLENPAERVHQI
QQILVTLPRVVIVVMRYLFAFLNHLSQYSDENMMDPYNLAICFGPTLMHI
PDGQDPVSCQAHINEVIKTIIIHHEAIFPSPRELEGPVYEKCMAGGEEYC
DSPHSEPGAIDEVDHDNGTEPHTSDEEVEQIEAIAKFDYMGRSPRELSFK
KGASLLLYHRASEDWWEGRHNGVDGLIPHQYIVVQDMDDAFSDSLSQKAD
SEASSGPLLDDKASSKNDLQSPTEHISDYGFGGVMGRVRLRSDGAAIPRR
RSGGDTHSPPRGLGPSIDTPPRAAACPSSPHKIPLTRGRIESPEKRRMAT
FGSAGSINYPDKKALSEGHSMRSTCGSTRHSSLGDHKSLEAEALAEDIEK
TMSTALHELRELERQNTVKQAPDVVLDTLEPLKNPPGPVSSEPASPLHTI
VIRDPDAAMRRSSSSSTEMMTTFKPALSARLAGAQLRPPPMRPVRPVVQH
RSSSSSSSGVGSPAVTPTEKMFPNSSADKSGTM*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11368
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 151697 Daltons