cDNA Clone RTT101 (yeast)

• Plasmid: pRS413 Pro-RTT101 (ATG=Nco1)
• Parent Plasmid: pRS413
• Genbank: Z49322
• Species: Yeast

Sequence of Insert

ACTAGTTCCAATTGTGAAGCAGACATGATCTGTTTGGCATATCCAATATTATAGAAATTGTACAAGTCTTTATTAAAAGCACTGATATCTTCTTCCATAGAGCTGGCCTTCGTCGTATCATAATCAGATTTACCTTCATCCTGCAAAATTGAAACGTTATCATTTGAAGGTGTAAACATGTTTTCATACATTGAGTTTATATCATGATATTTATCGTCTGCGTCTTTTGCCCATCGTGTAAGTGTGTGAAATAAAGTGGTGCAATGTATGAGGTTTCGCTGTCCCACATATCGAGGGGAGAGTGCC
CAATTACTTAGCATCATAGACGTAATGTAAACATCAAAGAAAAGTCTAGT
GTTTTCTTCTTTTGTATTCATCAAAAATTCTACACACGTGAGCTCGGAGA
TTTTTGGATGTTCAATATTCCTGATTAAGTATGACGAAGCGGAACGCATA
GCTATCCTTTGCGTAATGATGAATATGAACAATGCGAATGAAGATCATCG
ATATCTTTGCACTAAAAGACTACTTTGAAGGAATCCTCAAGATATTAAAC
CCACCAATCTTTTTTACTGGTATAAATTCTCGTACGGGTTCACAGGAACC
ATGGTAAATGAGAGCGTTTCCAAGAGAGAAGGATTCCACGAGTCTATAAG
CAGAGAGACGAGTGCAAGCAACGCTTTAGGACTATACAATAAATTTAACG
ATGAGCGAAATCCCAGGTATCGAACGATGATTGCTGAGCTGCACGAGTTT
TTTCACCTTACTTTAGCTGAAACTATAACTGAAACTGACGTGAAAGAATT
GGAGTGTAATAAAGAAAAGGCCGCTAAATTTAGAAAGCTAATGCCTAAGA
TGTTGAACAATTGCCGTGAATTAACGCAAAGGAAATCATACATTCCTTAC
AACTCAGAATTTAATGGGAATGACGAAAAGCAAAAGAAGTTTCAGCTGCT
ACATCAACACCAAATTGTGCTGAGTTTTCAAGAATTTTGTGATGAATTGG
CAAAGTTAATAATTGACGCGCACGTCCTTAGCTTTTTGACCAGATGCGAC
TATTCTTATGAAATTATACCCAAAAATTGGACCTCTTTTTACAAGCTATT
TCAGTACGTAATGGGTGCCGTTGGTCCTATTATATCGTATGTGCCGGTAA
ATTATCCCATGATAAGAAAAGAACTAGGATTTGAGACCTTAACGATATTT
CAGTATTATGATTCCAAACTTTTCGAATGTATGAAGTCACATTTTGGAAG
GGAATTTTCCACCCTTGTAAGCGCTACTATTCATCACTATATTCATATGT
TTCCAATAACGAATACAATGTTAGAGAAGGAAGTTCCTATGCTCCGTATA
ATGTCTAACTGCAATTTTAGTATAGAGGGCTTGAGCCCCAAAGATTTTTA
TATGAAAACTCTCAGACAATATTACTGTGAGGAATCAAATTTAAGGCCGA
GGCTGGAAACTTTCAAGAATTTTAAGGTACTATTGACGAGGAATGCATTA
TTAGCATCTTTATTTTCCCCTGAATGGGTCAGCGATGCAAATGACCTATT
TATAAGCCATCTACTGTTGAACAAGAAGTCTATTAGCGAATACATAGAAA
TTGGGAAGGACACTTATGATGAAGAAAAAGAGAGATATTTCAAAACTGAG
ACACACTTTAGCTTATTGATGTTTAGAAATGCTTTTGAAGCAAAAAATAT
GCTGTCAAAATTCAAGGAATTCTGTGATGATGCCGTTTCAGAAAAGTTAA
AGGCAGCATATGGATCCAATCATGATACTGAAAGGCTATTTGATGAGGTA
GTTCAGCTAGCTAATGTAGATCACCTCAAAATATACTCCGATTCCATCGA
GTACCATTTATGTAATTTACTTGGAAGTACCAGCAAAGCCATAGAACAGT
ATGTCAAATATTTTGAGTCACATTTATTCATCATCGTCAGAAAGATAAAA
ACAACTAAAAAAGACCTACCCAGAGATATGAAAATAAAGTATCTCAACGA
AAATCTTCCTATATTGAGACTGAAGTTCGTTAACCTTCCCACATTTCCTA
ATTTTTTCGAAAGATCAATTTTCAGAAAGACGATCTTACAAAGTGATCAA
AATTCCTCATTTATTAAGGATATATTACCTGTTTACAAAGACAGCCTCAT
GGAACTTTTCAAACAAAGAATCATAACAAATGTCTCACAAGAGGATGAAA
TGCGATACCGAGATCAATATCAACCCTATTTATCGCAATTCTTTCAGCCG
GTAGAGGTTATGGCAGATCTGAGAATCAAATATGCATCGTTCTTGTCGTT
TTATGAGAATATCGAGGCTGCTGTGAAGTTCGGGAAGACATACAATGAAA
ACAACAGCAAGTCATTTTTCCCACTTATCTTTGATAGAGAAAGAATTCCC
AAAGTTTTTCAGCAATCCAACGAAGTAAAGAAAAATTTTGTCCTACCTCA
AGAGATGGATGATACGTGGAACCAATTTCTCCGTAATTATCACGAGCAGA
ATAAAGTGGAAGATTCAGATGCTTCCAAAAAAGAGCTATATCCGATGTGG
AACTTGCACCACTGCGAAGTGGAGTCGCCTTATATCATACAGGATGGAAC
TAACCTAATATTTGAATTGACCCTTTTCCAAACATGTGTGCTGACTTTAT
TCAACGAAAGCGATCATTTGACCTTGCAAGTTATATCAGAGCAAACGAAA
CTGGCGTACAAAGACTTAGCGCTAGTCTTAAAATCCTTCTGCAATTATAA
AATTCTGACAAGAGATATTGACAACACATACAGTATCAACGAGAGCTTCA
AACCTGATATGAAAAAAGTGAAAAATGGGAAACTACGTGTGGTTCTACCG
AGAACCGCGTCTCTCCAAAGTAGCAACACAGGCGGTGAAAGAACCAGTTC
TGCACACCACGAAGGAAGTAATTCCCAATGGACCCAAGAATTACTAAAGG
CATGCATAACTAGATCCGTTAAGAGCGAGAGGAATGGGCTAGACTACGAC
CATTTATTTGAAACGGTAAAGCAGCAGATAAAAGGCTTTAGTGTTGGTGA
GTTCAAAGACGCCTTGGCCAAGTTGTTACGAGACAAATTCATAACTAGGG
ACGAATCAACAGCAACTTACAAGTACTAAGCGGCCGC

Amino Acid Sequence

MVNESVSKREGFHESISRETSASNALGLYNKFNDERNPRYRTMIAELHEF
FHLTLAETITETDVKELECNKEKAAKFRKLMPKMLNNCRELTQRKSYIPY
NSEFNGNDEKQKKFQLLHQHQIVLSFQEFCDELAKLIIDAHVLSFLTRCD
YSYEIIPKNWTSFYKLFQYVMGAVGPIISYVPVNYPMIRKELGFETLTIF
QYYDSKLFECMKSHFGREFSTLVSATIHHYIHMFPITNTMLEKEVPMLRI
MSNCNFSIEGLSPKDFYMKTLRQYYCEESNLRPRLETFKNFKVLLTRNAL
LASLFSPEWVSDANDLFISHLLLNKKSISEYIEIGKDTYDEEKERYFKTE
THFSLLMFRNAFEAKNMLSKFKEFCDDAVSEKLKAAYGSNHDTERLFDEV
VQLANVDHLKIYSDSIEYHLCNLLGSTSKAIEQYVKYFESHLFIIVRKIK
TTKKDLPRDMKIKYLNENLPILRLKFVNLPTFPNFFERSIFRKTILQSDQ
NSSFIKDILPVYKDSLMELFKQRIITNVSQEDEMRYRDQYQPYLSQFFQP
VEVMADLRIKYASFLSFYENIEAAVKFGKTYNENNSKSFFPLIFDRERIP
KVFQQSNEVKKNFVLPQEMDDTWNQFLRNYHEQNKVEDSDASKKELYPMW
NLHHCEVESPYIIQDGTNLIFELTLFQTCVLTLFNESDHLTLQVISEQTK
LAYKDLALVLKSFCNYKILTRDIDNTYSINESFKPDMKKVKNGKLRVVLP
RTASLQSSNTGGERTSSAHHEGSNSQWTQELLKACITRSVKSERNGLDYD
HLFETVKQQIKGFSVGEFKDALAKLLRDKFITRDESTATYKY*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11355
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast expression plasmid for untagged RTT101.; Sequence differences compared with ref Z49322:; a322g and t385c in promoter sequence - in all other rtt101 pro constructs.; I2V due to conversion of ATG to Nco1 site.