cDNA Clone Itch

• Plasmid: pCMV5 C-Myc-Itch
• Parent Plasmid: pCMV5
• Expressed: cMyc-Itch
• Genbank: NM_031483
• Tag: Myc
• Species: Human

Sequence of Insert

ggtaccgccaccatggaacaaaaacttatttctgaagaagatctgtctgacagtggatcacaacttggttcaatgggtagcctcaccatgaaatcacagcttcagatcactgtcatctcagcaaaacttaaggaaaataagaagaattggtttggaccaagtccttacgtagaggtcacagtagatggacagtcaaagaagacagaaaaatgcaacaacacaaacagtcccaagtggaagcaaccccttacagttatcgttacccctgtgagtaaattacattttcgtgtgtggagtcaccagacactgaaatctgatgttttgttgggaactgctgcattagatatttatgaaacattaaagtcaaacaatatgaaacttgaagaagtagttgtgactttgcagcttggaggtgacaaagagccaacagagacaataggagacttgtcaatttgtcttgatgggctacagttagagtctgaagttgttaccaatggtgaaactacatgttcagaaagtgcttctcagaatgatgatggctccagatccaaggatgaaacaagagtgagcacaaatggatcagatgaccctgaagatgcaggagctggtgaa
aataggagagtcagtgggaataattctccatcactctcaaatggtggttt
taaaccttctagacctccaagaccttcacgaccaccaccacccaccccac
gtagaccagcatctgtcaatggttcaccatctgccacttctgaaagtgat
gggtctagtacaggctctctgccgccgacaaatacaaatacaaatacatc
tgaaggagcaacatctggattaataattcctcttactatatctggaggct
caggccctaggccattaaatcctgtaactcaagctcccttgccacctggt
tgggagcagagagtggaccagcacgggcgagtttactatgtagatcatgt
tgagaaaagaacaacatgggatagaccagaacctctacctcctggctggg
aacggcgggttgacaacatgggacgtatttattatgttgaccatttcaca
agaacaacaacgtggcagaggccaacactggaatccgtccggaactatga
acaatggcagctacagcgtagtcagcttcaaggagcaatgcagcagttta
accagagattcatttatgggaatcaagatttatttgctacatcacaaagt
aaagaatttgatcctcttggtccattgccacctggatgggagaagagaac
agacagcaatggcagagtatatttcgtcaaccacaacacacgaattacac
aatgggaagaccccagaagtcaaggtcaattaaatgaaaagcccttacct
gaaggttgggaaatgagattcacagtggatggaattccatattttgtgga
ccacaatagaagaactaccacctatatagatccccgcacaggaaaatctg
ccctagacaatggacctcagatagcctatgttcgggacttcaaagcaaag
gttcagtatttccggttctggtgtcagcaactggccatgccacagcacat
aaagattacagtgacaagaaaaacattgtttgaggattcctttcaacaga
taatgagcttcagtccccaagatctgcgaagacgtttgtgggtgattttt
ccaggagaagaaggtttagattatggaggtgtagcaagagaatggttctt
tcttttgtcacatgaagtgttgaacccaatgtattgcctgtttgaatatg
cagggaaggataactactgcttgcagataaaccccgcttcttacatcaat
ccagatcacctgaaatattttcgttttattggcagattcattgccatggc
tctgttccatgggaaattcatagacacgggtttttctttaccattctata
agcgtatcttgaacaaaccagttggactcaaggatttagaatctattgat
ccagaattttacaattctctcatctgggttaaggaaaacaatattgagga
atgtgatttggaaatgtacttctccgttgacaaagaaattctaggtgaaa
ttaagagtcatgatctgaaacctaatggtggcaatattcttgtaacagaa
gaaaataaagaggaatacatcagaatggtagctgagtggaggttgtctcg
aggtgttgaagaacagacacaagctttctttgaaggctttaatgaaattc
ttccccagcaatatttgcaatactttgatgcaaaggaattagaggtcctt
ttatgtggaatgcaagagattgatttgaatgactggcaaagacatgccat
ctaccgtcattatgcaaggaccagcaaacaaatcatgtggttttggcagt
ttgttaaagaaattgataatgagaagagaatgagacttctgcagtttgtt
actggaacctgccgattgccagtaggaggatttgctgatctcatggggag
caatggaccacagaaattctgcattgaaaaagttgggaaagaaaattggc
tacccagaagtcatacctgttttaatcgcctggacctgccaccatacaag
agctatgagcaactgaaggaaaagctgttgtttgccatagaagaaacaga
aggatttggacaagagtaaggatcc

Amino Acid Sequence

MEQKLISEEDLSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPS
PYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLK
SDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLD
GLQLESEVVTNGETTCSESASQNDDGSRSKDETRVSTNGSDDPEDAGAGE
NRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESD
GSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPG
WEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFT
RTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQS
KEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLP
EGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAK
VQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIF
PGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYIN
PDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESID
PEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTE
ENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVL
LCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFV
TGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYK
SYEQLKEKLLFAIEETEGFGQE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11352
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Nucleotide sequence difference compared with NM_031483 CDS: ; t1809c (silent in F603); ; Estimated MW of expressed protein : 99859 Daltons