cDNA Clone RTT101 (yeast)

• Plasmid: pRS306 PRO-15HA-RTT101 (M1-G216)
• Parent Plasmid: pRS306
• Genbank: Z49322
• Tag: HA

Sequence of Insert

DNA sequence of promoter
TGGTGGATAAACTTTGTATAACAAATCTGCCCTTTGCCTTTACTATATTTTCCAATTGTGAAGCAGACATGATCTGTTTGGCATATCCAATATTATAGAAATTGTACAAGTCTTTATTAAAAGCACTGATATCTTCTTCCATAGAGCTGGCCTTCGTCGTATCATAATCAGATTTACCTTCATCCTGCAAAATTGAAACGTTATCATTTGAAGGTGTAAACATGTTTTCATACATTGAGTTTATATCATGATATTTATCGTCTGCGTCTTTTGCCCATCGTGTAAGTGTGTGAAATAAAGTGGTGCAATGTATGAGGTTTCGCTGTCCCACATATCGAGGGGAGAGTGCCCAATTACTTAGCATCATAGACGTAATGTAAACATCAAAGAAAAGTCTAGTGTTTTCTTCTTTTGTATTCATCAAAAATTCTACACACGTGAGCTCGGAGATTTTTGGATGTTCAATATTCCTGATTAAGTATGACGAAGCGGAACGCATAGCTATCCTTTGCGTAATGATGAATATGAACAATGCGAATGAAGATCATCGATATCTTTGCACTAAAAGACTACTTTGAAGGAATCCTCAAGATATTAAACCCACCAATCTTTTTTACTGGTATAAATTCTCGTACGGGTTCACAGGAACC

DNA sequence of 15HA-RTT101 fragment
ATGGGCTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGT
CCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATTACGCTGCTC
AGTGCGGCCGCATCTTTTACCCATACGATGTTCCTGACTATGCGGGCTAT
CCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGA
TTACGCTGCTATGGGCTACCCATACGATGTTCCTGACTATGCGGGCTATC
CCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGAT
TACGCTGCTATGGGCTACCCATACGATGTTCCTGACTATGCGGGCTATCC
CTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATT
ACGCTGCTATGGGCTACCCATACGATGTTCCTGACTATGCGGGCTATCCC
TATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATTA
CGCTGCCATGGTAAATGAGAGCGTTTCCAAGAGAGAAGGATTCCACGAGT
CTATAAGCAGAGAGACGAGTGCAAGCAACGCTTTAGGACTATACAATAAA
TTTAACGATGAGCGAAATCCCAGGTATCGAACGATGATTGCTGAGCTGCA
CGAGTTTTTTCACCTTACTTTAGCTGAAACTATAACTGAAACTGACGTGA
AAGAATTGGAGTGTAATAAAGAAAAGGCCGCTAAATTTAGAAAGCTAATG
CCTAAGATGTTGAACAATTGCCGTGAATTAACGCAAAGGAAATCATACAT
TCCTTACAACTCAGAATTTAATGGGAATGACGAAAAGCAAAAGAAGTTTC
AGCTGCTACATCAACACCAAATTGTGCTGAGTTTTCAAGAATTTTGTGAT
GAATTGGCAAAGTTAATAATTGACGCGCACGTCCTTAGCTTTTTGACCAG
ATGCGACTATTCTTATGAAATTATACCCAAAAATTGGACCTCTTTTTACA
AGCTATTTCAGTACGTAATGGGTGCCGTTGGTCCTATTATATCGTATGTG
CCGGTAAATTATCCCATGATAAGAAAAGAACTAGGATTTGAGACCTTAAC
GATATTTCAGTATTATGATTCCAAACTTTTCGAATGTATGAAGTCACATT
TTGGAAG

Amino Acid Sequence

MGYPYDVPDYAGYPYDVPDYAGSYPYDVPDYAAQCGRIFYPYDVPDYAGY
PYDVPDYAGSYPYDVPDYAAMGYPYDVPDYAGYPYDVPDYAGSYPYDVPD
YAAMGYPYDVPDYAGYPYDVPDYAGSYPYDVPDYAAMGYPYDVPDYAGYP
YDVPDYAGSYPYDVPDYAAMVNESVSKREGFHESISRETSASNALGLYNK
FNDERNPRYRTMIAELHEFFHLTLAETITETDVKELECNKEKAAKFRKLM
PKMLNNCRELTQRKSYIPYNSEFNGNDEKQKKFQLLHQHQIVLSFQEFCD
ELAKLIIDAHVLSFLTRCDYSYEIIPKNWTSFYKLFQYVMGAVGPIISYV
PVNYPMIRKELGFETLTIFQYYDSKLFECMKSHFG

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11326
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence differences in promoter sequence compared with reference:; a372g and t435c; J. Rouse notified.; Parental Plasmid DU11273