cDNA Clone RTT101 (yeast)

• Plasmid: pGEX6P1-RTT101 (yeast)
• Parent Plasmid: pGEX6P1
• Expressed: GST-C3 RTT101 (yeast)
• Genbank: Z49322
• Tag: GST
• Species: Yeast

Sequence of Insert

GTCGACTCATGATAAATGAGAGCGTTTCCAAGAGAGAAGGATTCCACGAGTCTATAAGCAGAGAGACGAGTGCAAGCAACGCTTTAGGACTATACAATAAATTTAACGATGAGCGAAATCCCAGGTATCGAACGATGATTGCTGAGCTGCACGAGTTTTTTCACCTTACTTTAGCTGAAACTATAACTGAAACTGACGTGAAAGAATTGGAGTGTAATAAAGAAAAGGCCGCTAAATTTAGAAAGCTAATGCCTAAGATGTTGAACAATTGCCGTGAATTAACGCAAAGGAAATCATACATTCCTTACAACTCAGAATTTAATGGGAATGACGAAAAGCAAAAGAAGTTTCAGCTGCTACATCAACACCAAATTGTGCTGAGTTTTCAAGAATTTTGTGATGAATTGG
CAAAGTTAATAATTGACGCGCACGTCCTTAGCTTTTTGACCAGATGCGAC
TATTCTTATGAAATTATACCCAAAAATTGGACCTCTTTTTACAAGCTATT
TCAGTACGTAATGGGTGCCGTTGGTCCTATTATATCGTATGTGCCGGTAA
ATTATCCCATGATAAGAAAAGAACTAGGATTTGAGACCTTAACGATATTT
CAGTATTATGATTCCAAACTTTTCGAATGTATGAAGTCACATTTTGGAAG
GGAATTTTCCACCCTTGTAAGCGCTACTATTCATCACTATATTCATATGT
TTCCAATAACGAATACAATGTTAGAGAAGGAAGTTCCTATGCTCCGTATA
ATGTCTAACTGCAATTTTAGTATAGAGGGCTTGAGCCCCAAAGATTTTTA
TATGAAAACTCTCAGACAATATTACTGTGAGGAATCAAATTTAAGGCCGA
GGCTGGAAACTTTCAAGAATTTTAAGGTACTATTGACGAGGAATGCATTA
TTAGCATCTTTATTTTCCCCTGAATGGGTCAGCGATGCAAATGACCTATT
TATAAGCCATCTACTGTTGAACAAGAAGTCTATTAGCGAATACATAGAAA
TTGGGAAGGACACTTATGATGAAGAAAAAGAGAGATATTTCAAAACTGAG
ACACACTTTAGCTTATTGATGTTTAGAAATGCTTTTGAAGCAAAAAATAT
GCTGTCAAAATTCAAGGAATTCTGTGATGATGCCGTTTCAGAAAAGTTAA
AGGCAGCATATGGATCCAATCATGATACTGAAAGGCTATTTGATGAGGTA
GTTCAGCTAGCTAATGTAGATCACCTCAAAATATACTCCGATTCCATCGA
GTACCATTTATGTAATTTACTTGGAAGTACCAGCAAAGCCATAGAACAGT
ATGTCAAATATTTTGAGTCACATTTATTCATCATCGTCAGAAAGATAAAA
ACAACTAAAAAAGACCTACCCAGAGATATGAAAATAAAGTATCTCAACGA
AAATCTTCCTATATTGAGACTGAAGTTCGTTAACCTTCCCACATTTCCTA
ATTTTTTCGAAAGATCAATTTTCAGAAAGACGATCTTACAAAGTGATCAA
AATTCCTCATTTATTAAGGATATATTACCTGTTTACAAAGACAGCCTCAT
GGAACTTTTCAAACAAAGAATCATAACAAATGTCTCACAAGAGGATGAAA
TGCGATACCGAGATCAATATCAACCCTATTTATCGCAATTCTTTCAGCCG
GTAGAGGTTATGGCAGATCTGAGAATCAAATATGCATCGTTCTTGTCGTT
TTATGAGAATATCGAGGCTGCTGTGAAGTTCGGGAAGACATACAATGAAA
ACAACAGCAAGTCATTTTTCCCACTTATCTTTGATAGAGAAAGAATTCCC
AAAGTTTTTCAGCAATCCAACGAAGTAAAGAAAAATTTTGTCCTACCTCA
AGAGATGGATGATACGTGGAACCAATTTCTCCGTAATTATCACGAGCAGA
ATAAAGTGGAAGATTCAGATGCTTCCAAAAAAGAGCTATATCCGATGTGG
AACTTGCACCACTGCGAAGTGGAGTCGCCTTATATCATACAGGATGGAAC
TAACCTAATATTTGAATTGACCCTTTTCCAAACATGTGTGCTGACTTTAT
TCAACGAAAGCGATCATTTGACCTTGCAAGTTATATCAGAGCAAACGAAA
CTGGCGTACAAAGACTTAGCGCTAGTCTTAAAATCCTTCTGCAATTATAA
AATTCTGACAAGAGATATTGACAACACATACAGTATCAACGAGAGCTTCA
AACCTGATATGAAAAAAGTGAAAAATGGGAAACTACGTGTGGTTCTACCG
AGAACCGCGTCTCTCCAAAGTAGCAACACAGGCGGTGAAAGAACCAGTTC
TGCACACCACGAAGGAAGTAATTCCCAATGGACCCAAGAATTACTAAAGG
CATGCATAACTAGATCCGTTAAGAGCGAGAGGAATGGGCTAGACTACGAC
CATTTATTTGAAACGGTAAAGCAGCAGATAAAAGGCTTTAGTGTTGGTGA
GTTCAAAGACGCCTTGGCCAAGTTGTTACGAGACAAATTCATAACTAGGG
ACGAATCAACAGCAACTTACAAGTACTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFPGRLMINESVSKREGF
HESISRETSASNALGLYNKFNDERNPRYRTMIAELHEFFHLTLAETITET
DVKELECNKEKAAKFRKLMPKMLNNCRELTQRKSYIPYNSEFNGNDEKQK
KFQLLHQHQIVLSFQEFCDELAKLIIDAHVLSFLTRCDYSYEIIPKNWTS
FYKLFQYVMGAVGPIISYVPVNYPMIRKELGFETLTIFQYYDSKLFECMK
SHFGREFSTLVSATIHHYIHMFPITNTMLEKEVPMLRIMSNCNFSIEGLS
PKDFYMKTLRQYYCEESNLRPRLETFKNFKVLLTRNALLASLFSPEWVSD
ANDLFISHLLLNKKSISEYIEIGKDTYDEEKERYFKTETHFSLLMFRNAF
EAKNMLSKFKEFCDDAVSEKLKAAYGSNHDTERLFDEVVQLANVDHLKIY
SDSIEYHLCNLLGSTSKAIEQYVKYFESHLFIIVRKIKTTKKDLPRDMKI
KYLNENLPILRLKFVNLPTFPNFFERSIFRKTILQSDQNSSFIKDILPVY
KDSLMELFKQRIITNVSQEDEMRYRDQYQPYLSQFFQPVEVMADLRIKYA
SFLSFYENIEAAVKFGKTYNENNSKSFFPLIFDRERIPKVFQQSNEVKKN
FVLPQEMDDTWNQFLRNYHEQNKVEDSDASKKELYPMWNLHHCEVESPYI
IQDGTNLIFELTLFQTCVLTLFNESDHLTLQVISEQTKLAYKDLALVLKS
FCNYKILTRDIDNTYSINESFKPDMKKVKNGKLRVVLPRTASLQSSNTGG
ERTSSAHHEGSNSQWTQELLKACITRSVKSERNGLDYDHLFETVKQQIKG
FSVGEFKDALAKLLRDKFITRDESTATYKY*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11315
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion