cDNA Clone RTT101 (yeast)

• Plasmid: pETBlue2.6His RTT101
• Parent Plasmid: pETBlue2 6His
• Genbank: Z49322
• Tag: 6His
• Species: Yeast

Sequence of Insert

GTCGACTCATGATAAATGAGAGCGTTTCCAAGAGAGAAGGATTCCACGAGTCTATAAGCAGAGAGACGAGTGCAAGCAACGCTTTAGGACTATACAATAAATTTAACGATGAGCGAAATCCCAGGTATCGAACGATGATTGCTGAGCTGCACGAGTTTTTTCACCTTACTTTAGCTGAAACTATAACTGAAACTGACGTGAAAGAATTGGAGTGTAATAAAGAAAAGGCCGCTAAATTTAGAAAGCTAATGCCTAAGATGTTGAACAATTGCCGTGAATTAACGCAAAGGAAATCATACATTCCTTACAACTCAGAATTTAATGGGAATGACGAAAAGCAAAAGAAGTTTCAGCTGCTACATCAACACCAAATTGTGCTGAGTTTTCAAGAATTTTGTGATGAATTGG
CAAAGTTAATAATTGACGCGCACGTCCTTAGCTTTTTGACCAGATGCGAC
TATTCTTATGAAATTATACCCAAAAATTGGACCTCTTTTTACAAGCTATT
TCAGTACGTAATGGGTGCCGTTGGTCCTATTATATCGTATGTGCCGGTAA
ATTATCCCATGATAAGAAAAGAACTAGGATTTGAGACCTTAACGATATTT
CAGTATTATGATTCCAAACTTTTCGAATGTATGAAGTCACATTTTGGAAG
GGAATTTTCCACCCTTGTAAGCGCTACTATTCATCACTATATTCATATGT
TTCCAATAACGAATACAATGTTAGAGAAGGAAGTTCCTATGCTCCGTATA
ATGTCTAACTGCAATTTTAGTATAGAGGGCTTGAGCCCCAAAGATTTTTA
TATGAAAACTCTCAGACAATATTACTGTGAGGAATCAAATTTAAGGCCGA
GGCTGGAAACTTTCAAGAATTTTAAGGTACTATTGACGAGGAATGCATTA
TTAGCATCTTTATTTTCCCCTGAATGGGTCAGCGATGCAAATGACCTATT
TATAAGCCATCTACTGTTGAACAAGAAGTCTATTAGCGAATACATAGAAA
TTGGGAAGGACACTTATGATGAAGAAAAAGAGAGATATTTCAAAACTGAG
ACACACTTTAGCTTATTGATGTTTAGAAATGCTTTTGAAGCAAAAAATAT
GCTGTCAAAATTCAAGGAATTCTGTGATGATGCCGTTTCAGAAAAGTTAA
AGGCAGCATATGGATCCAATCATGATACTGAAAGGCTATTTGATGAGGTA
GTTCAGCTAGCTAATGTAGATCACCTCAAAATATACTCCGATTCCATCGA
GTACCATTTATGTAATTTACTTGGAAGTACCAGCAAAGCCATAGAACAGT
ATGTCAAATATTTTGAGTCACATTTATTCATCATCGTCAGAAAGATAAAA
ACAACTAAAAAAGACCTACCCAGAGATATGAAAATAAAGTATCTCAACGA
AAATCTTCCTATATTGAGACTGAAGTTCGTTAACCTTCCCACATTTCCTA
ATTTTTTCGAAAGATCAATTTTCAGAAAGACGATCTTACAAAGTGATCAA
AATTCCTCATTTATTAAGGATATATTACCTGTTTACAAAGACAGCCTCAT
GGAACTTTTCAAACAAAGAATCATAACAAATGTCTCACAAGAGGATGAAA
TGCGATACCGAGATCAATATCAACCCTATTTATCGCAATTCTTTCAGCCG
GTAGAGGTTATGGCAGATCTGAGAATCAAATATGCATCGTTCTTGTCGTT
TTATGAGAATATCGAGGCTGCTGTGAAGTTCGGGAAGACATACAATGAAA
ACAACAGCAAGTCATTTTTCCCACTTATCTTTGATAGAGAAAGAATTCCC
AAAGTTTTTCAGCAATCCAACGAAGTAAAGAAAAATTTTGTCCTACCTCA
AGAGATGGATGATACGTGGAACCAATTTCTCCGTAATTATCACGAGCAGA
ATAAAGTGGAAGATTCAGATGCTTCCAAAAAAGAGCTATATCCGATGTGG
AACTTGCACCACTGCGAAGTGGAGTCGCCTTATATCATACAGGATGGAAC
TAACCTAATATTTGAATTGACCCTTTTCCAAACATGTGTGCTGACTTTAT
TCAACGAAAGCGATCATTTGACCTTGCAAGTTATATCAGAGCAAACGAAA
CTGGCGTACAAAGACTTAGCGCTAGTCTTAAAATCCTTCTGCAATTATAA
AATTCTGACAAGAGATATTGACAACACATACAGTATCAACGAGAGCTTCA
AACCTGATATGAAAAAAGTGAAAAATGGGAAACTACGTGTGGTTCTACCG
AGAACCGCGTCTCTCCAAAGTAGCAACACAGGCGGTGAAAGAACCAGTTC
TGCACACCACGAAGGAAGTAATTCCCAATGGACCCAAGAATTACTAAAGG
CATGCATAACTAGATCCGTTAAGAGCGAGAGGAATGGGCTAGACTACGAC
CATTTATTTGAAACGGTAAAGCAGCAGATAAAAGGCTTTAGTGTTGGTGA
GTTCAAAGACGCCTTGGCCAAGTTGTTACGAGACAAATTCATAACTAGGG
ACGAATCAACAGCAACTTACAAGTACTAAGCGGCCGC

Amino Acid Sequence

MGHHHHHHLGSPEFPGRLMINESVSKREGFHESISRETSASNALGLYNKF
NDERNPRYRTMIAELHEFFHLTLAETITETDVKELECNKEKAAKFRKLMP
KMLNNCRELTQRKSYIPYNSEFNGNDEKQKKFQLLHQHQIVLSFQEFCDE
LAKLIIDAHVLSFLTRCDYSYEIIPKNWTSFYKLFQYVMGAVGPIISYVP
VNYPMIRKELGFETLTIFQYYDSKLFECMKSHFGREFSTLVSATIHHYIH
MFPITNTMLEKEVPMLRIMSNCNFSIEGLSPKDFYMKTLRQYYCEESNLR
PRLETFKNFKVLLTRNALLASLFSPEWVSDANDLFISHLLLNKKSISEYI
EIGKDTYDEEKERYFKTETHFSLLMFRNAFEAKNMLSKFKEFCDDAVSEK
LKAAYGSNHDTERLFDEVVQLANVDHLKIYSDSIEYHLCNLLGSTSKAIE
QYVKYFESHLFIIVRKIKTTKKDLPRDMKIKYLNENLPILRLKFVNLPTF
PNFFERSIFRKTILQSDQNSSFIKDILPVYKDSLMELFKQRIITNVSQED
EMRYRDQYQPYLSQFFQPVEVMADLRIKYASFLSFYENIEAAVKFGKTYN
ENNSKSFFPLIFDRERIPKVFQQSNEVKKNFVLPQEMDDTWNQFLRNYHE
QNKVEDSDASKKELYPMWNLHHCEVESPYIIQDGTNLIFELTLFQTCVLT
LFNESDHLTLQVISEQTKLAYKDLALVLKSFCNYKILTRDIDNTYSINES
FKPDMKKVKNGKLRVVLPRTASLQSSNTGGERTSSAHHEGSNSQWTQELL
KACITRSVKSERNGLDYDHLFETVKQQIKGFSVGEFKDALAKLLRDKFIT
RDESTATYKY*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11295
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct