cDNA Clone CDC53 (yeast)

• Plasmid: pETBlue2.6His CDC53
• Parent Plasmid: pETBlue2 6His
• Tag: 6His
• Species: Yeast

Sequence of Insert

GGATCCATGTCTGAGACTCTGCCTAGATCTGATGATTTAGAGGCCACTTGGAACTTTATAGAGCCAGGAATTAACCAAATACTGGGTAATGAGAAAAACCAAGCATCCACGTCTAAGCGAGTTTACAAAATTCTTTCTCCAACCATGTATATGGAAGTCTATACCGCAATTTATAACTACTGTGTGAACAAATCACGCTCGTCTGGACATTTTAGTACTGACAGTAGAACTGGCCAATCAACAATTTTGGTTGGCAGTGAGATTTACGAAAAGTTAAAGAATTATTTGAAGAATTACATTTTAAAT
TTTAAGCAGTCTAATTCAGAGACCTTTTTGCAGTTTTACGTCAAGCGCTG
GAAAAGGTTTACAATAGGTGCCATTTTTTTAAACCATGCATTCGATTATA
TGAATAGATATTGGGTTCAAAAGGAAAGAAGTGACGGCAAAAGGCATATT
TTTGATGTAAACACCTTGTGCTTGATGACATGGAAAGAAGTCATGTTTGA
TCCGAGTAAAGATGTTTTAATAAACGAATTATTAGACCAGGTAACCTTGG
GAAGGGAGGGGCAAATAATTCAAAGAAGTAATATTAGCACTGCCATAAAG
TCTTTAGTTGCACTAGGTATCGATCCACAGGATTTGAAAAAGTTGAACCT
AAATGTCTACATCCAAGTTTTCGAAAAGCCATTTTTAAAGAAGACTCAGG
AGTACTACACGCAATATACAAACGATTATTTAGAGAAACACTCGGTAACT
GAGTATATTTTTGAAGCACATGAAATCATCAAACGTGAGGAAAAAGCAAT
GACAATATATTGGGATGATCATACAAAAAAACCACTATCCATGGCATTAA
ACAAGGTCTTGATCACAGACCATATTGAAAAGTTGGAAAATGAGTTTGTT
GTCCTTCTGGATGCCAGAGATATTGAAAAAATTACTTCTTTGTACGCACT
AATACGCAGAGACTTTACATTAATCCCAAGAATGGCTTCAGTGTTTGAAA
ATTATGTTAAAAAGACAGGTGAGAATGAAATTTCGAGTCTACTGGCAATG
CATAAACACAATATTATGAAAAACGAAAACGCAAACCCTAAAAAACTAGC
ACTAATGACAGCTCACTCCCTTTCTCCTAAAGACTATATCAAGAAATTAC
TGGAAGTACACGATATATTCTCTAAGATTTTTAATGAAAGTTTCCCCGAC
GACATACCTTTAGCTAAAGCTCTAGATAATGCGTGTGGTGCTTTCATCAA
CATCAACGAGTTCGCATTACCTGCTGGATCTCCAAAAAGTGCCACCTCGA
AGACTTCCGAGATGCTAGCTAAGTACAGTGATATACTATTAAAGAAGGCC
ACCAAACCTGAAGTGGCAAGTGACATGTCAGATGAAGATATTATAACAAT
ATTCAAATATTTGACCGACAAAGATGCGTTCGAAACTCATTATAGAAGAC
TTTTCGCCAAGCGTTTAATTCATGGCACTTCAACATCAGCAGAAGACGAA
GAAAATATTATTCAAAGGCTGCAAGCGGCAAATAGTATGGAATATACAGG
CAAGATAACTAAAATGTTTCAAGATATTAGACTTTCCAAGATCTTGGAAG
ACGATTTTGCTGTCGCCCTGAAGAATGAACCAGATTACTCTAAAGCAAAA
TATCCAGATCTACAACCATTTGTATTGGCAGAAAATATGTGGCCATTTTC
ATACCAAGAAGTTGAATTTAAGCTACCCAAGGAATTGGTACCATCTCACG
AAAAATTGAAGGAGTCATACAGCCAAAAGCATAATGGTAGAATATTGAAG
TGGCTATGGCCATTATGTCGTGGCGAATTGAAGGCAGATATTGGAAAACC
CGGCAGGATGCCGTTCAACTTTACGGTAACACTGTTTCAAATGGCAATTT
TGCTACTATATAACGATGCTGATGTATTAACCTTAGAAAATATTCAAGAG
GGCACGAGCTTAACCATTCAGCACATCGCAGCGGCAATGGTACCATTCAT
CAAATTCAAACTGATTCAACAGGTCCCACCAGGGCTAGATGCTCTCGTGA
AACCTGAAACACAATTCAAGCTATCACGTCCTTATAAGGCGCTTAAGACC
AATATTAATTTCGCTAGCGGAGTCAAAAACGACATTCTGCAATCGCTATC
CGGTGGAGGCCACGATAATCACGGTAATAAGTTAGGAAACAAACGCTTGA
CGGAAGACGAAAGAATAGAAAAAGAATTGAATACAGAAAGGCAGATTTTC
TTGGAAGCTTGTATTGTGAGAATAATGAAAGCGAAAAGAAATCTGCCACA
TACAACATTGGTCAACGAATGCATTGCGCAATCACACCAAAGGTTCAATG
CAAAGGTTTCTATGGTCAAGAGAGCCATCGATAGCTTAATACAAAAGGGA
TACCTACAGAGGGGAGACGATGGTGAATCGTATGCTTACCTTGCTTAAGCGGCCGC

Amino Acid Sequence

MGHHHHHHLGSMSETLPRSDDLEATWNFIEPGINQILGNEKNQASTSKRV
YKILSPTMYMEVYTAIYNYCVNKSRSSGHFSTDSRTGQSTILVGSEIYEK
LKNYLKNYILNFKQSNSETFLQFYVKRWKRFTIGAIFLNHAFDYMNRYWV
QKERSDGKRHIFDVNTLCLMTWKEVMFDPSKDVLINELLDQVTLGREGQI
IQRSNISTAIKSLVALGIDPQDLKKLNLNVYIQVFEKPFLKKTQEYYTQY
TNDYLEKHSVTEYIFEAHEIIKREEKAMTIYWDDHTKKPLSMALNKVLIT
DHIEKLENEFVVLLDARDIEKITSLYALIRRDFTLIPRMASVFENYVKKT
GENEISSLLAMHKHNIMKNENANPKKLALMTAHSLSPKDYIKKLLEVHDI
FSKIFNESFPDDIPLAKALDNACGAFININEFALPAGSPKSATSKTSEML
AKYSDILLKKATKPEVASDMSDEDIITIFKYLTDKDAFETHYRRLFAKRL
IHGTSTSAEDEENIIQRLQAANSMEYTGKITKMFQDIRLSKILEDDFAVA
LKNEPDYSKAKYPDLQPFVLAENMWPFSYQEVEFKLPKELVPSHEKLKES
YSQKHNGRILKWLWPLCRGELKADIGKPGRMPFNFTVTLFQMAILLLYND
ADVLTLENIQEGTSLTIQHIAAAMVPFIKFKLIQQVPPGLDALVKPETQF
KLSRPYKALKTNINFASGVKNDILQSLSGGGHDNHGNKLGNKRLTEDERI
EKELNTERQIFLEACIVRIMKAKRNLPHTTLVNECIAQSHQRFNAKVSMV
KRAIDSLIQKGYLQRGDDGESYAYLA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11294
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct