cDNA Clone Ubr1 (yeast)

• Plasmid: pETBlue2.6His Ubr1
• Parent Plasmid: pETBlue2 6His
• Tag: 6His
• Species: Yeast

Sequence of Insert

GGATCCATGTCCGTTGCTGATGATGATTTAGGATCTTTACAAGGTCACATTAGGAGAACACTGAGGTCTATTCATAACCTCCCCTATTTTAGGTATACGAGAGGTCCTACTGAAAGGGCTGACATGAGCAGAGCCCTTAAAGAGTTCATTTACAGATATCTATACTTTGTCATTTCTAACAGCGGAGAGAACTTACCTACTTTATTCAATGCTCATCCAAAACAAAAATTATCTAACCCAGAGCTTACTGTTTTTCCTGACAGTTTAGAAGATGCTGTGGATATTGATAAGATAACATCTCAACAA
ACTATTCCGTTTTATAAGATAGATGAATCCAGAATAGGAGACGTCCATAA
ACATACCGGAAGAAATTGTGGGAGGAAATTCAAAATAGGGGAACCCTTGT
ATAGGTGTCATGAGTGTGGTTGCGATGATACTTGTGTGCTTTGTATTCAT
TGTTTTAATCCAAAAGATCATGTGAATCATCATGTTTGTACCGATATATG
TACTGAATTCACTAGTGGAATTTGTGACTGTGGAGATGAAGAAGCTTGGA
ATTCTCCACTTCATTGCAAAGCTGAAGAACAAGAAAACGATATATCGGAA
GATCCCGCCACAAATGCCGACATAAAGGAAGAAGATGTATGGAATGATTC
AGTGAATATAGCATTAGTGGAGTTGGTTCTTGCAGAAGTGTTTGACTATT
TTATCGATGTTTTTAACCAGAATATCGAACCTCTACCCACAATACAGAAA
GATATCACTATCAAATTAAGAGAGATGACACAACAGGGAAAAATGTATGA
AAGAGCGCAGTTTTTAAACGATTTAAAATATGAAAACGATTATATGTTTG
ACGGAACAACAACAGCAAAGACGAGCCCCTCTAATAGCCCTGAGGCATCT
CCATCGCTTGCGAAAATCGATCCCGAAAATTACACGGTAATAATATATAA
CGATGAATATCATAACTATTCGCAGGCTACGACTGCCTTGAGACAGGGGG
TTCCAGATAACGTACACATTGATCTATTAACATCCAGGATTGACGGAGAA
GGTCGAGCAATGTTGAAATGTTCACAAGATTTATCATCTGTCTTAGGTGG
TTTTTTTGCTGTTCAAACGAATGGATTAAGTGCCACTTTAACATCGTGGT
CAGAATATCTTCATCAAGAAACATGCAAGTATATCATCCTTTGGATTACA
CACTGTTTAAACATACCAAACTCCTCCTTCCAAACCACTTTTAGGAACAT
GATGGGTAAAACCCTGTGTTCAGAATACTTAAATGCAACAGAATGTCGTG
ATATGACACCGGTGGTGGAGAAATATTTCAGCAACAAGTTCGATAAGAAT
GATCCCTACAGATACATCGATTTATCTATCCTTGCAGATGGCAATCAAAT
TCCTTTGGGTCATCACAAAATTCTACCCGAATCAAGCACCCATTCACTTT
CACCATTGATAAATGATGTAGAAACTCCAACATCAAGAACATATTCTAAT
ACAAGATTACAACATATTCTTTATTTTGATAACAGATATTGGAAAAGACT
GAGAAAAGATATTCAGAATGTGATCATTCCAACGTTGGCCTCTTCAAATT
TATACAAGCCCATTTTCTGTCAACAGGTAGTCGAAATATTCAACCACATC
ACAAGATCTGTGGCATATATGGATAGAGAACCTCAGCTAACAGCTATAAG
AGAGTGTGTCGTACAGTTATTTACATGCCCTACGAATGCCAAAAATATTT
TCGAAAACCAAAGTTTTTTGGATATTGTGTGGTCAATTATAGACATTTTT
AAGGAGTTTTGTAAGGTGGAAGGTGGTGTATTGATCTGGCAGAGAGTTCA
AAAAAGCAATTTAACAAAGAGTTACAGTATATCATTCAAGCAAGGGTTGT
ATACAGTTGAAACTTTATTGAGTAAGGTACATGATCCTAATATTCCATTG
AGACCTAAGGAAATAATATCTCTATTAACTCTTTGCAAGCTCTTCAATGG
AGCGTGGAAGATAAAGCGTAAAGAAGGTGAACATGTTCTTCACGAAGATC
AAAATTTCATCTCCTATTTAGAATATACCACCTCCATTTACAGTATTATT
CAAACAGCGGAAAAAGTTAGCGAAAAATCAAAAGATTCCATTGATTCAAA
GCTTTTTTTAAATGCAATAAGAATTATCAGTTCATTTCTAGGCAATAGAT
CACTAACATACAAGTTAATATATGATTCTCATGAAGTCATTAAGTTTAGC
GTAAGCCACGAAAGAGTTGCATTTATGAATCCTTTACAAACAATGCTTTC
ATTCCTTATCGAAAAAGTGTCTTTAAAGGATGCATACGAAGCGTTAGAAG
ACTGTTCAGATTTTCTGAAAATATCGGATTTCTCTTTGAGATCAGTTGTA
TTGTGCTCTCAAATTGACGTCGGGTTTTGGGTAAGAAATGGTATGTCTGT
GTTACATCAAGCATCGTATTATAAAAACAATCCCGAATTAGGTTCCTATT
CCAGAGATATACATTTGAATCAGTTAGCTATCCTTTGGGAACGTGATGAC
ATTCCGAGAATAATATACAATATTCTTGATAGATGGGAATTACTAGACTG
GTTTACAGGTGAAGTTGACTATCAACACACTGTCTATGAGGATAAAATTT
CATTCATCATTCAACAATTCATTGCCTTTATTTATCAAATATTGACCGAA
AGACAATATTTTAAAACTTTCTCTTCTCTAAAGGATAGAAGAATGGATCA
AATCAAAAATTCAATCATTTATAACCTTTACATGAAACCCCTTTCGTATT
CAAAGCTACTAAGATCAGTTCCTGACTACCTAACTGAAGATACTACCGAG
TTTGACGAAGCACTTGAAGAAGTCTCTGTTTTCGTTGAGCCTAAGGGCTT
GGCCGACAATGGTGTTTTTAAATTAAAAGCAAGCTTGTATGCAAAGGTTG
ATCCGTTAAAGTTGCTGAATTTGGAAAACGAATTCGAAAGTAGTGCAACT
ATTATTAAATCACATTTGGCTAAGGATAAGGATGAAATAGCAAAAGTTGT
ACTAATACCCCAAGTCTCAATAAAACAGTTAGATAAAGACGCTCTCAATC
TGGGTGCTTTTACAAGGAATACGGTATTTGCCAAAGTGGTCTATAAACTA
CTCCAGGTATGTCTGGATATGGAGGATAGCACTTTCCTGAATGAGCTACT
ACATTTAGTTCATGGTATATTCAGGGATGATGAATTGATAAATGGTAAGG
ACTCTATACCAGAAGCTTATTTGTCAAAGCCTATATGTAATTTACTCTTG
AGCATAGCCAATGCTAAATCAGACGTATTTTCCGAGAGTATTGTCCGTAA
AGCCGACTATTTGTTAGAGAAGATGATAATGAAAAAACCAAATGAACTTT
TTGAGTCATTAATTGCCAGTTTTGGCAACCAATACGTTAATGATTATAAG
GATAAGAAACTACGCCAAGGAGTTAACTTACAAGAGACAGAGAAGGAACG
AAAAAGAAGATTGGCCAAGAAACATCAAGCAAGGCTTCTAGCCAAGTTTA
ACAATCAACAAACGAAATTCATGAAAGAACATGAGTCTGAATTTGATGAG
CAGGATAACGATGTTGACATGGTCGGTGAAAAAGTATATGAATCGGAGGA
TTTTACCTGCGCACTATGTCAAGATTCCAGTTCGACCGATTTTTTTGTGA
TACCTGCGTACCATGATCATTCCCCGATATTTAGACCTGGTAATATTTTT
AACCCAAATGAATTTATGCCCATGTGGGATGGTTTCTACAACGATGATGA
AAAACAAGCTTATATTGACGATGATGTTTTAGAGGCTTTGAAGGAAAACG
GTAGTTGTGGATCTAGGAAAGTATTTGTATCCTGTAATCATCACATTCAT
CATAACTGCTTCAAGAGGTACGTTCAAAAAAAGAGATTTTCATCAAACGC
GTTTATATGTCCTTTATGCCAAACATTTTCGAATTGTACTTTACCTCTTT
GTCAAACCTCAAAGGCAAATACTGGTTTGTCGCTAGATATGTTTTTGGAA
TCAGAACTTTCTTTGGATACATTGTCAAGGCTCTTCAAACCTTTTACTGA
GGAAAATTATCGTACAATAAACTCTATATTTTCGTTGATGATATCTCAGT
GTCAAGGTTTTGATAAAGCGGTAAGAAAGCGGGCCAACTTTTCGCATAAG
GACGTTTCTTTAATATTAAGTGTGCACTGGGCAAATACCATTTCAATGTT
AGAAATTGCTTCTAGGTTAGAAAAGCCATACAGTATTTCATTTTTCAGAA
GCAGGGAACAGAAATACAAAACTTTAAAAAATATATTAGTCTGCATTATG
CTGTTCACCTTTGTCATAGGCAAGCCAAGTATGGAGTTTGAACCGTACCC
GCAGCAGCCCGATACTGTTTGGAACCAAAATCAACTCTTCCAATATATTG
TTCGAAGCGCTTTATTTTCTCCAGTTTCACTTCGTCAGACTGTTACAGAG
GCGTTAACAACGTTCTCTAGACAGTTTCTAAGAGATTTTCTTCAGGGTCT
TTCTGATGCAGAACAGGTTACCAAATTGTATGCTAAAGCGTCCAAAATTG
GGGATGTTCTTAAAGTAAGTGAGCAGATGCTCTTCGCCCTAAGGACCATA
TCTGATGTACGAATGGAGGGATTGGACTCCGAAAGTATTATTTATGATCT
AGCTTATACCTTCCTGTTGAAGAGTCTTCTTCCTACGATAAGAAGGTGCT
TAGTCTTTATCAAAGTGCTACACGAATTAGTGAAAGATTCTGAAAATGAA
ACGCTGGTCATCAACGGTCATGAGGTTGAAGAAGAACTGGAATTCGAAGA
TACCGCCGAGTTTGTCAATAAGGCATTGAAAATGATTACTGAAAAAGAAT
CCTTAGTCGATCTTTTGACAACGCAGGAGTCCATTGTATCGCATCCATAT
TTGGAGAACATCCCATATGAATATTGTGGGATTATAAAACTGATAGATTT
ATCAAAATATTTGAATACTTACGTTACCCAATCTAAGGAAATCAAGCTAC
GTGAAGAGCGTTCTCAGCATATGAAGAATGCGGATAATCGATTAGATTTC
AAAATATGTTTGACATGTGGTGTCAAAGTGCATTTAAGAGCGGACCGCCA
TGAAATGACCAAGCATTTGAATAAAAATTGTTTTAAACCATTTGGCGCCT
TCCTGATGCCCAATTCAAGTGAAGTTTGTCTCCATTTGACACAGCCTCCT
TCTAATATTTTTATATCTGCACCATATCTAAATTCGCATGGTGAAGTTGG
TAGAAATGCCATGAGAAGAGGTGATTTAACCACCTTGAATTTGAAAAGAT
ATGAGCACTTAAACAGATTATGGATTAACAATGAAATTCCAGGTTATATT
AGTAGAGTAATGGGTGACGAATTTCGAGTGACTATACTTTCCAATGGTTT
CTTGTTTGCATTTAATAGAGAACCAAGGCCAAGAAGAATACCTCCAACAG
ATGAAGATGATGAAGATATGGAAGAGGGAGAAGACGGGTTTTTTACGGAG
GGAAATGACGAAATGGATGTTGATGATGAAACTGGACAGGCCGCCAACTT
ATTTGGGGTTGGCGCAGAAGGTATTGCTGGTGGCGGAGTACGTGATTTTT
TCCAATTCTTCGAAAACTTTAGGAACACTCTACAACCGCAGGGAAATGGC
GATGATGATGCACCTCAGAATCCCCCACCAATATTGCAGTTCTTAGGACC
TCAATTTGATGGAGCTACTATCATAAGAAATACAAATCCAAGAAACCTCG
ATGAGGATGACTCTGATGATAATGATGACTCTGATGAGCGAGAGATTTGG
TAGCGGCCGC

Amino Acid Sequence

MGHHHHHHLGSMSVADDDLGSLQGHIRRTLRSIHNLPYFRYTRGPTERAD
MSRALKEFIYRYLYFVISNSGENLPTLFNAHPKQKLSNPELTVFPDSLED
AVDIDKITSQQTIPFYKIDESRIGDVHKHTGRNCGRKFKIGEPLYRCHEC
GCDDTCVLCIHCFNPKDHVNHHVCTDICTEFTSGICDCGDEEAWNSPLHC
KAEEQENDISEDPATNADIKEEDVWNDSVNIALVELVLAEVFDYFIDVFN
QNIEPLPTIQKDITIKLREMTQQGKMYERAQFLNDLKYENDYMFDGTTTA
KTSPSNSPEASPSLAKIDPENYTVIIYNDEYHNYSQATTALRQGVPDNVH
IDLLTSRIDGEGRAMLKCSQDLSSVLGGFFAVQTNGLSATLTSWSEYLHQ
ETCKYIILWITHCLNIPNSSFQTTFRNMMGKTLCSEYLNATECRDMTPVV
EKYFSNKFDKNDPYRYIDLSILADGNQIPLGHHKILPESSTHSLSPLIND
VETPTSRTYSNTRLQHILYFDNRYWKRLRKDIQNVIIPTLASSNLYKPIF
CQQVVEIFNHITRSVAYMDREPQLTAIRECVVQLFTCPTNAKNIFENQSF
LDIVWSIIDIFKEFCKVEGGVLIWQRVQKSNLTKSYSISFKQGLYTVETL
LSKVHDPNIPLRPKEIISLLTLCKLFNGAWKIKRKEGEHVLHEDQNFISY
LEYTTSIYSIIQTAEKVSEKSKDSIDSKLFLNAIRIISSFLGNRSLTYKL
IYDSHEVIKFSVSHERVAFMNPLQTMLSFLIEKVSLKDAYEALEDCSDFL
KISDFSLRSVVLCSQIDVGFWVRNGMSVLHQASYYKNNPELGSYSRDIHL
NQLAILWERDDIPRIIYNILDRWELLDWFTGEVDYQHTVYEDKISFIIQQ
FIAFIYQILTERQYFKTFSSLKDRRMDQIKNSIIYNLYMKPLSYSKLLRS
VPDYLTEDTTEFDEALEEVSVFVEPKGLADNGVFKLKASLYAKVDPLKLL
NLENEFESSATIIKSHLAKDKDEIAKVVLIPQVSIKQLDKDALNLGAFTR
NTVFAKVVYKLLQVCLDMEDSTFLNELLHLVHGIFRDDELINGKDSIPEA
YLSKPICNLLLSIANAKSDVFSESIVRKADYLLEKMIMKKPNELFESLIA
SFGNQYVNDYKDKKLRQGVNLQETEKERKRRLAKKHQARLLAKFNNQQTK
FMKEHESEFDEQDNDVDMVGEKVYESEDFTCALCQDSSSTDFFVIPAYHD
HSPIFRPGNIFNPNEFMPMWDGFYNDDEKQAYIDDDVLEALKENGSCGSR
KVFVSCNHHIHHNCFKRYVQKKRFSSNAFICPLCQTFSNCTLPLCQTSKA
NTGLSLDMFLESELSLDTLSRLFKPFTEENYRTINSIFSLMISQCQGFDK
AVRKRANFSHKDVSLILSVHWANTISMLEIASRLEKPYSISFFRSREQKY
KTLKNILVCIMLFTFVIGKPSMEFEPYPQQPDTVWNQNQLFQYIVRSALF
SPVSLRQTVTEALTTFSRQFLRDFLQGLSDAEQVTKLYAKASKIGDVLKV
SEQMLFALRTISDVRMEGLDSESIIYDLAYTFLLKSLLPTIRRCLVFIKV
LHELVKDSENETLVINGHEVEEELEFEDTAEFVNKALKMITEKESLVDLL
TTQESIVSHPYLENIPYEYCGIIKLIDLSKYLNTYVTQSKEIKLREERSQ
HMKNADNRLDFKICLTCGVKVHLRADRHEMTKHLNKNCFKPFGAFLMPNS
SEVCLHLTQPPSNIFISAPYLNSHGEVGRNAMRRGDLTTLNLKRYEHLNR
LWINNEIPGYISRVMGDEFRVTILSNGFLFAFNREPRPRRIPPTDEDDED
MEEGEDGFFTEGNDEMDVDDETGQAANLFGVGAEGIAGGGVRDFFQFFEN
FRNTLQPQGNGDDDAPQNPPPILQFLGPQFDGATIIRNTNPRNLDEDDSD
DNDDSDEREIW*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11293
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct