cDNA Clone Claspin

• Plasmid: pGEX6P-2-Claspin
• Parent Plasmid: pGEX6P2
• Expressed: GST-Claspin
• Genbank: NM_022111
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGACAGGCGAGGTGGGTTCTGAGGTTCACCTAGAAATCAATGACCCAAACGTCATTTCACAAGAGGAAGCAGATAGTCCTTCAGATAGTGGACAGGGCAGCTATGAAACAATTGGACCCTTGAGTGAAGGAGATTCAGATGAAGAGATATTTGTAAGTAAGAAGTTGAAAAACAGGAAGGTTCTACAAGACAGTGATTCCGAAACAGAGGACACAAATGCCTCTCCAGAGAAAACTACCTATGACAGTGCCGAGGAGGAAAATAAAGAGAATTTATATGCTGGGAAAAATACAAAAATC
AAAAGGATTTACAAAACTGTGGCAGACAGTGATGAAAGTTACATGGAAAA
GTCTTTGTATCAGGAAAATCTTGAAGCGCAAGTGAAACCTTGCTTAGAGC
TGAGTCTTCAGTCTGGAAACTCTACAGACTTTACCACTGACAGAAAGAGT
TCCAAAAAGCACATACATGATAAAGAAGGAACTGCAGGAAAAGCAAAAGT
AAAATCAAAAAGAAGACTTGAGAAAGAGGAGAGAAAAATGGAAAAAATTA
GACAGCTAAAAAAGAAGGAAACAAAAAACCAGGAAGATGATGTAGAACAG
CCATTTAATGACAGTGGCTGTCTTCTTGTGGATAAAGACCTTTTTGAAAC
TGGGTTGGAGGATGAAAATAACTCTCCATTGGAAGATGAAGAGTCATTAG
AATCAATAAGAGCAGCTGTAAAAAACAAAGTAAAAAAGCACAAGAAAAAA
GAACCATCTTTGGAGAGTGGGGTCCATTCATTTGAGGAAGGAAGTGAGTT
ATCAAAAGGAACCACGAGGAAGGAAAGAAAGGCAGCCAGATTAAGTAAAG
AAGCATTAAAACAACTGCATAGTGAGACTCAGCGCCTTATTCGAGAGTCT
GCACTGAACCTTCCATATCATATGCCTGAGAATAAAACCATTCATGATTT
CTTCAAACGTAAACCCCGGCCCACTTGCCACGGAAATGCCATGGCACTAT
TGAAGTCATCTAAATATCAGTCAAGCCATCACAAAGAAATCATAGACACT
GCAAATACTACTGAAATGAACAGTGATCACCATAGTAAAGGTTCTGAGCA
GACAACAGGTGCAGAAAATGAAGTGGAAACTAATGCACTCCCTGTAGTTT
CAAAGGAAACCCAGATCATTACTGGATCAGATGAGTCTTGCAGGAAGGAT
TTGGTAAAAAATGAAGAGCTAGAAATTCAGGAGAAACAGAAGCAGAGTGA
CATTAGACCTTCACCTGGGGACAGCTCAGTGTTGCAACAGGAATCCAACT
TCCTCGGGAACAATCACAGTGAGGAATGTCAGGTTGGAGGGCTTGTAGCA
TTTGAACCTCATGCCCTGGAGGGTGAAGGCCCCCAAAATCCAGAAGAAAC
AGATGAGAAAGTGGAAGAGCCTGAGCAGCAAAATAAATCATCAGCAGTTG
GGCCACCTGAAAAAGTGAGACGGTTTACTCTGGATAGACTTAAGCAACTG
GGAGTAGATGTTTCCATTAAACCACGGCTAGGTGCTGATGAAGATTCCTT
TGTGATACTTGAACCTGAAACCAACAGAGAACTGGAAGCCTTGAAGCAGC
GTTTCTGGAAGCATGCTAATCCAGCAGCCAAACCCAGGGCTGGTCAGACA
GTGAATGTGAACGTCATAGTGAAAGACATGGGCACTGATGGAAAGGAAGA
GCTAAAAGCAGATGTGGTACCTGTGACTTTAGCACCTAAGAAGTTGGATG
GGGCAAGCCACACAAAACCAGGTGAAAAGCTTCAGGTGTTAAAAGCTAAA
CTGCAAGAAGCAATGAAACTCCGAAGGTTTGAGGAGCGCCAGAAGCGCCA
AGCACTGTTTAAATTAGATAATGAAGATGGGTTTGAGGAAGAGGAGGAGG
AAGAGGAAGAAATGACAGATGAGTCTGAGGAAGATGGAGAAGAGAAGGTA
GAGAAAGAAGAGAAAGAGGAAGAACTAGAGGAAGAGGAGGAGAAAGAAGA
GGAGGAGGAAGAAGAAGGAAATCAGGAGACTGCAGAATTCCTTCTTAGTA
GTGAAGAAATAGAAACAAAAGATGAAAAAGAAATGGATAAAGAAAATAAT
GATGGCAGTAGTGAAATTGGCAAGGCAGTTGGCTTCCTCTCTGTTCCCAA
GTCTCTCTCATCAGATTCTACTTTACTTCTGTTTAAGGACAGCTCTTCCA
AGATGGGTTACTTTCCTACTGAAGAAAAATCAGAAACAGATGAAAACTCA
GGCAAGCAGCCTAGCAAACTGGATGAGGATGATTCATGTTCATTGCTAAC
AAAGGAGAGCAGCCACAATAGCAGCTTTGAGCTGATTGGCTCCACGATTC
CATCCTATCAGCCTTGCAACAGACAAACAGGCCGTGGGACCAGTTTTTTC
CCTACAGCAGGAGGATTCAGATCTCCTTCCCCTGGGCTATTTCGAGCCAG
TTTGGTCAGCTCAGCTTCTAAGAGTTCAGGGAAACTGTCTGAGCCTTCAC
TTCCCATAGAGGATTCCCAGGATCTGTATAACGCCTCCCCAGAGCCTAAG
ACACTTTTCCTAGGAGCAGGAGACTTCCAGTTCTGTTTAGAAGATGACAC
TCAGAGCCAACTGTTGGATGCAGATGGGTTCTTAAATGTTAGAAACCACA
GGAATCAGTACCAAGCTTTGAAGCCTCGATTGCCATTGGCCAGTATGGAT
GAGAATGCCATGGATGCCAACATGGATGAGCTGTTGGATTTGTGTACTGG
AAAGTTCACATCTCAGGCTGAAAAACATCTACCCAGGAAGAGTGACAAGA
AAGAGAACATGGAGGAACTTCTGAACCTTTGTTCAGGAAAATTCACTTCT
CAGGATGCCTCCACTCCAGCCTCATCAGAGTTAAATAAACAGGAGAAGGA
GAGCAGCATGGGTGATCCAATGGAAGAAGCACTTGCTCTTTGCTCAGGCT
CTTTTCCCACAGACAAGGAAGAGGAAGACGAGGAGGAGGAATTTGGAGAC
TTTCGGCTTGTTTCAAATGATAATGAGTTTGATAGTGATGAGGATGAACA
CAGTGACTCTGGTAATGATCTGGCACTGGAAGACCATGAAGATGATGATG
AAGAAGAACTCCTGAAGCGATCTGAGAAGTTGAAAAGGCAAATGAGGTTG
AGGAAATACCTGGAGGATGAGGCAGAGGTGTCAGGAAGTGATGTGGGAAG
CGAAGATGAGTATGATGGGGAAGAAATTGATGAATATGAAGAGGACGTAA
TTGATGAAGTACTTCCTTCTGATGAGGAACTGCAGAGTCAAATCAAGAAA
ATACACATGAAAACTATGTTGGATGATGATAAGCGACAGCTACGTTTATA
CCAAGAGAGGTACCTTGCTGATGGGGATCTGCACAGCGATGGTCCTGGGC
GAATGAGGAAGTTTCGATGGAAAAACATAGATGATGCTTCCCAGATGGAC
TTGTTCCACAGAGACTCTGATGATGATCAGACTGAAGAACAGCTTGATGA
GTCAGAAGCCAGGTGGAGGAAGGAGCGAATTGAACGAGAGCAGTGGCTTC
GGGACATGGCACAGCAGGGGAAAATTACAGCTGAAGAAGAAGAAGAAATT
GGGGAGGACAGTCAGTTTATGATACTGGCCAAGAAAGTTACAGCCAAAGC
ACTGCAGAAGAATGCCAGTCGCCCTATGGTTATTCAGGAATCAAAGTCTT
TGCTCAGAAATCCTTTTGAAGCCATCAGACCAGGAAGTGCTCAACAGGTG
AAGACAGGCTCACTGCTAAACCAGCCCAAAGCTGTGCTTCAGAAACTGGC
TGCTCTCTCTGACCATAACCCCAGTGCTCCTCGAAATTCAAGAAACTTTG
TCTTTCATACACTTTCTCCTGTCAAGGCTGAGGCGGCAAAGGAATCGTCT
AAGTCTCAGGTAAAGAAAAGGGGTCCATCTTTCATGACTTCTCCTTCACC
TAAGCACCTCAAAACAGATGATAGCACTTCAGGATTGACGCGAAGCATCT
TCAAATATTTGGAGAGCTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMTGEVGSEVHLEINDPNVI
SQEEADSPSDSGQGSYETIGPLSEGDSDEEIFVSKKLKNRKVLQDSDSET
EDTNASPEKTTYDSAEEENKENLYAGKNTKIKRIYKTVADSDESYMEKSL
YQENLEAQVKPCLELSLQSGNSTDFTTDRKSSKKHIHDKEGTAGKAKVKS
KRRLEKEERKMEKIRQLKKKETKNQEDDVEQPFNDSGCLLVDKDLFETGL
EDENNSPLEDEESLESIRAAVKNKVKKHKKKEPSLESGVHSFEEGSELSK
GTTRKERKAARLSKEALKQLHSETQRLIRESALNLPYHMPENKTIHDFFK
RKPRPTCHGNAMALLKSSKYQSSHHKEIIDTANTTEMNSDHHSKGSEQTT
GAENEVETNALPVVSKETQIITGSDESCRKDLVKNEELEIQEKQKQSDIR
PSPGDSSVLQQESNFLGNNHSEECQVGGLVAFEPHALEGEGPQNPEETDE
KVEEPEQQNKSSAVGPPEKVRRFTLDRLKQLGVDVSIKPRLGADEDSFVI
LEPETNRELEALKQRFWKHANPAAKPRAGQTVNVNVIVKDMGTDGKEELK
ADVVPVTLAPKKLDGASHTKPGEKLQVLKAKLQEAMKLRRFEERQKRQAL
FKLDNEDGFEEEEEEEEEMTDESEEDGEEKVEKEEKEEELEEEEEKEEEE
EEEGNQETAEFLLSSEEIETKDEKEMDKENNDGSSEIGKAVGFLSVPKSL
SSDSTLLLFKDSSSKMGYFPTEEKSETDENSGKQPSKLDEDDSCSLLTKE
SSHNSSFELIGSTIPSYQPCNRQTGRGTSFFPTAGGFRSPSPGLFRASLV
SSASKSSGKLSEPSLPIEDSQDLYNASPEPKTLFLGAGDFQFCLEDDTQS
QLLDADGFLNVRNHRNQYQALKPRLPLASMDENAMDANMDELLDLCTGKF
TSQAEKHLPRKSDKKENMEELLNLCSGKFTSQDASTPASSELNKQEKESS
MGDPMEEALALCSGSFPTDKEEEDEEEEFGDFRLVSNDNEFDSDEDEHSD
SGNDLALEDHEDDDEEELLKRSEKLKRQMRLRKYLEDEAEVSGSDVGSED
EYDGEEIDEYEEDVIDEVLPSDEELQSQIKKIHMKTMLDDDKRQLRLYQE
RYLADGDLHSDGPGRMRKFRWKNIDDASQMDLFHRDSDDDQTEEQLDESE
ARWRKERIEREQWLRDMAQQGKITAEEEEEIGEDSQFMILAKKVTAKALQ
KNASRPMVIQESKSLLRNPFEAIRPGSAQQVKTGSLLNQPKAVLQKLAAL
SDHNPSAPRNSRNFVFHTLSPVKAEAAKESSKSQVKKRGPSFMTSPSPKH
LKTDDSTSGLTRSIFKYLES*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11285
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; Sequence difference compared with NM_022111 CDS:; a1752g (silent) - originates from EST 7961377 used for amplification.; ; Estimated MW of expressed protein : 177913 Daltons