cDNA Clone TBC1D1

• Synonyms: TBC; TBC1
• Plasmid: pCMV5-HA TBC1D1 R125W
• Parent Plasmid: pCMV5
• Expressed: HA-TBC1D1 R125W
• Genbank: NM_015173.3 GI:359807029 with silent change c1266a
• Tag: HA
• Species: Human

Sequence of Insert

GGTACCGCCACCATGTACCCATACGATGTGCCAGATTACGCCATGGAACCAATAACATTCACAGCAAGGAAACATCTGCTTTCTAACGAGGTCTCGGTGGATTTTGGCCTGCAGCTGGTGGGCTCCCTGCCTGTGCATTCCCTGACCACCATGCCCATGCTGCCCTGGGTTGTGGCTGAGGTGCGAAGACTCAGCAGGCAGTCCACCAGAAAGGAACCTGTAACCAAGCAAGTCCGGCTTTGCGTTTCACCCTCTGGACTGAGATGTGAACCTGAGCCAGGGAGAAGTCAACAGTGGGATCCCCTGATCTATTCCAGCATCTTTGAGTGCAAGCCTCAGCGTGTTCACAAACTGATTCACAACAGTCATGACCCAAGTTACTTTGCTTGTCTGATTAAGGAAGACGCTGTCCACTGGCAGAGTATCTGCTATGTGTTCAAAGCCGATGATCAAACAAAAGTGCCTGAGATCATCAGCTCCATCCGTCAGGCGGGGAAGATCGCCCGGCAGGAGGAGCTGCACTGCCCGTCCGAGTTCGACGACACGTTTTCCAAGAAGTTCGAGGTGCTCTTCTGCGGCCGCGTGACGGTGGCGCACAAGAAGGCTCCGCCGGCCCTGATCGACGAGTGCATCGAGAAGTTCAATCACGTCAGCGGCAGCCGGGGGTCCGAGAGCCCCCGCCCCAACCCGCCCCATGCCGCGCCCACAGGGAGCCAGGAGCCTGTGCGCAGGCCCATGCGCAAGTCCTTCTCCCAGCCCGGCCTGCGCTCGCTGGCCTTTAGGAAGGAGCTGCAGGATGGGGGCCTCCGAAGCAGCGGCTTCTTCAGCTCCTTCGAGGAGAGCGACATTGAGAACCACCTCATTAGCGGACACAATATTGTGCAGCCCACAGATATCGAGGAAAATCGAACTATGCTCTTCACGATTGGCCAGTCTGAAGTTTACCTCATCAGTCCTGACACCAAAAAAATAGCATTGGAGAAAAATTTTAAGGAGATATCCTTTTGCTCTCAGGGCATCAGACACGTGGACCACTTTGGGTTTATCTGTCGGGAGTCTTCCGGAGGTGGCGGCTTTCATTTTGTCTGTTACGTGTTTCAGTGCACAAATGAGGCTCTGGTTGATGAAATTATGATGACCCTGAAACAGGCCTTCACGGTGGCCGCAGTGCAGCAGACAGCTAAGGCGCCAGCCCAGCTGTGTGAGGGCTGCCCCCTGCAAAGCCTGCACAAGCTCTGTGAGAGGATAGAGGGAATGAATTCTTCCAAAACAAAACTAGAACTGCAAAAGCACCTGACGACATTAACAAATCAGGAGCAGGCGACTATTTTTGAAGAGGTTCAGAAATTGAGACCGAGAAATGAGCAGCGAGAGAATGAATTGATTATTTCTTTTCTGAGATGTTTATATGAAGAGAAACAGAAAGAACACATCCATATTGGGGAGATGAAGCAGACATCGCAGATGGCAGCAGAGAATATTGGAAGTGAATTACCACCCAGTGCCACTCGATTTAGGCTAGATATGCTGAAAAACAAAGCAAAGAGATCTTTAACAGAGTCTTTAGAAAGTATTTTGTCCCGGGGTAATAAAGCCAGAGGCCTGCAGGAACACTCCATCAGTGTGGATCTGGATAGCTCCCTGTCTAGTACATTAAGTAACACCAGCAAAGAGCCATCTGTGTGTGAAAAGGAGGCCTTGCCCATCTCTGAGAGCTCCTTTAAGCTCCTCGGCTCCTCGGAGGACCTGTCCAGTGACTCGGAGAGTCATCTCCCAGAAGAGCCAGCTCCGCTGTCGCCCCAGCAGGCCTTCAGGAGGCGAGCAAACACCCTGAGTCACTTCCCCATCGAATGCCAGGAACCTCCACAACCTGCCCGGGGGTCCCCGGGGGTTTCGCAAAGGAAACTTATGAGGTATCACTCAGTGAGCACAGAGACGCCTCATGAACGAAAGGACTTTGAATCCAAAGCAAACCATCTTGGTGATTCTGGTGGGACTCCTGTGAAGACCCGGAGGCATTCCTGGAGGCAGCAGATATTCCTCCGAGTAGCCACCCCGCAGAAGGCGTGCGATTCTTCCAGCAGATATGAAGATTATTCAGAGCTGGGAGAGCTTCCCCCACGATCTCCTTTAGAACCAGTTTGTGAAGATGGGCCCTTTGGCCCCCCACCAGAGGAAAAGAAAAGGACATCTCGTGAGCTCCGAGAGCTGTGGCAAAAGGCTATTCTTCAACAGATACTGCTGCTTAGAATGGAGAAGGAAAATCAGAAGCTCCAAGCCTCTGAAAATGATTTGCTGAACAAGCGCCTGAAGCTCGATTATGAAGAAATTACTCCCTGTCTTAAAGAAGTAACTACAGTGTGGGAAAAGATGCTTAGCACTCCAGGAAGATCAAAAATTAAGTTTGACATGGAAAAAATGCACTCGGCTGTTGGGCAAGGTGTGCCACGTCATCACCGAGGTGAAATCTGGAAATTTCTAGCTGAGCAATTCCACCTTAAACACCAGTTTCCCAGCAAACAGCAGCCAAAGGATGTGCCATACAAAGAACTCTTAAAGCAGCTGACTTCCCAGCAGCATGCGATTCTTATTGACCTTGGGCGAACCTTTCCTACACACCCATACTTCTCTGCCCAGCTTGGAGCAGGACAGCTATCGCTTTACAACATTTTGAAGGCCTACTCACTTCTAGACCAGGAAGTGGGATATTGCCAAGGTCTCAGCTTTGTAGCAGGCATTTTGCTTCTTCATATGAGTGAGGAAGAGGCGTTTAAAATGCTCAAGTTTCTGATGTTTGACATGGGGCTGCGGAAACAGTATCGGCCAGACATGATTATTTTACAGATCCAGATGTACCAGCTCTCGAGGTTGCTTCATGATTACCACAGAGACCTCTACAATCACCTGGAGGAGCACGAGATCGGCCCCAGCCTCTACGCTGCCCCCTGGTTCCTCACCATGTTTGCCTCACAGTTCCCGCTGGGATTCGTAGCCAGAGTCTTTGATATGATTTTTCTTCAGGGAACAGAGGTCATATTTAAAGTGGCTTTAAGTCTGTTGGGAAGCCATAAGCCCTTGATTCTGCAGCATGAAAACCTAGAAACCATAGTTGACTTTATAAAAAGCACGCTACCCAACCTTGGCTTGGTACAGATGGAAAAGACCATCAATCAGGTATTTGAAATGGACATCGCTAAACAGTTACAAGCTTATGAAGTTGAGTACCACGTCCTTCAAGAAGAACTTATCGATTCCTCTCCTCTCAGTGACAACCAAAGAATGGATAAATTAGAGAAAACCAACAGCAGCTTACGCAAACAGAACCTTGACCTCCTTGAACAGTTGCAGGTGGCAAATGGTAGGATCCAAAGCCTTGAGGCCACCATTGAGAAGCTCCTGAGCAGTGAGAGCAAGCTGAAGCAGGCCATGCTTACCTTAGAACTGGAGCGGTCGGCCCTGCTGCAGACGGTGGAGGAGCTGCGGCGGCGGAGCGCAGAGCCCAGCGACCGGGAGCCTGAGTGCACGCAGCCCGAGCCCACGGGCGACTGAGTCGAC

Amino Acid Sequence

MYPYDVPDYAMEPITFTARKHLLSNEVSVDFGLQLVGSLPVHSLTTMPML
PWVVAEVRRLSRQSTRKEPVTKQVRLCVSPSGLRCEPEPGRSQQWDPLIY
SSIFECKPQRVHKLIHNSHDPSYFACLIKEDAVHWQSICYVFKADDQTKV
PEIISSIRQAGKIARQEELHCPSEFDDTFSKKFEVLFCGRVTVAHKKAPP
ALIDECIEKFNHVSGSRGSESPRPNPPHAAPTGSQEPVRRPMRKSFSQPG
LRSLAFRKELQDGGLRSSGFFSSFEESDIENHLISGHNIVQPTDIEENRT
MLFTIGQSEVYLISPDTKKIALEKNFKEISFCSQGIRHVDHFGFICRESS
GGGGFHFVCYVFQCTNEALVDEIMMTLKQAFTVAAVQQTAKAPAQLCEGC
PLQSLHKLCERIEGMNSSKTKLELQKHLTTLTNQEQATIFEEVQKLRPRN
EQRENELIISFLRCLYEEKQKEHIHIGEMKQTSQMAAENIGSELPPSATR
FRLDMLKNKAKRSLTESLESILSRGNKARGLQEHSISVDLDSSLSSTLSN
TSKEPSVCEKEALPISESSFKLLGSSEDLSSDSESHLPEEPAPLSPQQAF
RRRANTLSHFPIECQEPPQPARGSPGVSQRKLMRYHSVSTETPHERKDFE
SKANHLGDSGGTPVKTRRHSWRQQIFLRVATPQKACDSSSRYEDYSELGE
LPPRSPLEPVCEDGPFGPPPEEKKRTSRELRELWQKAILQQILLLRMEKE
NQKLQASENDLLNKRLKLDYEEITPCLKEVTTVWEKMLSTPGRSKIKFDM
EKMHSAVGQGVPRHHRGEIWKFLAEQFHLKHQFPSKQQPKDVPYKELLKQ
LTSQQHAILIDLGRTFPTHPYFSAQLGAGQLSLYNILKAYSLLDQEVGYC
QGLSFVAGILLLHMSEEEAFKMLKFLMFDMGLRKQYRPDMIILQIQMYQL
SRLLHDYHRDLYNHLEEHEIGPSLYAAPWFLTMFASQFPLGFVARVFDMI
FLQGTEVIFKVALSLLGSHKPLILQHENLETIVDFIKSTLPNLGLVQMEK
TINQVFEMDIAKQLQAYEVEYHVLQEELIDSSPLSDNQRMDKLEKTNSSL
RKQNLDLLEQLQVANGRIQSLEATIEKLLSSESKLKQAMLTLELERSALL
QTVEELRRRSAEPSDREPECTQPEPTGD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11274
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee