cDNA Clone ESC4 (yeast)

• Plasmid: pRS415 13Myc RTTFL 743/758/773/806A
• Parent Plasmid: pRS415
• Tag: 13Myc
• Species: Yeast

Amino Acid Sequence

MAGRRIPGLINGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLN
GEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDL
NGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEED
LNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLINHTMGTSLLFEQLNF
LILVAAEAELPIAHSTRKLLMDNSCNNCQIYELYNENLKDVKTDKDWFMN
KFGPQTVHFVISNTINFPFYKIVYFDLLIPVVSHTWVQDSVKTKRHLRTN
MYSPNPFHLLRDCQVYISKSSFNKCEYILYSDLLHLLGGTLVNYISNRTT
HVIVQSPQDPIIATVSKLTFGSFSSSSTNKHTEKPLREWKFVYPIWILYH
FKMAKPLKGELATLCELDMQDTSEEQLFAKWEEVIGDKQTSSSQLTLHPN
KTLFKNHHFAISPDLNFFTPLYWFLKGFIEDLDGKVTPLSFSDDLKSVYQ
AFPDIDCYIGHSANSPILEKTKSIKPEIHVGNVSWLFYMFALQKFTPVSQ
CKLIHQPFHAKLFTSKELTVAYTNYFGSQRFYIQRLVEILGGLSTPELTR
KNTHLITKSTIGKKFKVAKKWSLDPQNAIIVTNHMWLEQCYMNNSKLNPK
DSRFQNFKLDDNMGWNIGQIGMDHSSLPTPKNLSMVTYDTQSISEKPPPT
NDQMDQINDNTNVLSKKDGTPISSFENSIDEKIDKLQKISGEVAVTHSGD
LERSFVSRPSRASFPVVDSKKSNLQKKDSNSDISMETEVFCEGHEKREEK
EFTKPITEYDAPKKQEIREQSRKKNDIDYKKEEEETELQVQLGQRTKREI
KTSKKNEKEKETNECHIEVDQMTNEKQGEESTGKLISTEDVTSKKDTDKF
SHLFEGLSDNDDHINDEKPAVNSKYTTPKTAQNITSGVDTPTTAQAQVFM
PSSGNSRLAKAQAAKRLHTDIESLNEFQKNFKRKRIDSEEISLAQDVERS
NNNKELATKAEKILARFNELPNYDLKAVCTGCFHDGFNEVDIEILNQLGI
KIFDNIKETDKLNCIFAPKILRTEKFLKSLSFEPLKFALKPEFIIDLLKQ
IHSKKDKLSQININLFDYEINGINESIISKTKLPTKVFERANIRCINLVN
DIPGGVDTIGSVLKAHGIEKINVLRSKKCTFEDIIPNDVSKQENGGIFKY
VLIVTKASQVKKFTKLINDRDKNETILIVEWNWCVESIFHLNVDFTSKKN
VLYQKKNN*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11272
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast non-intergrating expression plasmid.; Sequence differences (other than intended mutations) in ESC4 compared with reference CDS:; a4g (S2G) (resulting from generation of Nco1 site); t3006g (silent in S1002)