cDNA Clone TBC1D1

• Plasmid: pCMV5-FLAG TBC1D1
• Parent Plasmid: pCMV5
• Expressed: FLAG-TBC1D1
• Genbank: NM_015173
• Tag: FLAG
• Species: Human

Sequence of Insert

ggtaccgccaccATGGACTACAAGGACGACGATGACAAGatggaaccaataacattcacagcaaggaaacatctgctttctaacgaggtctcggtggattttggcctgcagctggtgggctccctgcctgtgcattccctgaccaccatgcccatgctgccctgggttgtggctgaggtgcgaagactcagcaggcagtccaccagaaaggaacctgtaaccaagcaagtccggctttgcgtttcaccctctggactgagatgtgaacctgagccagggagaagtcaacagtgggatcccctgatctattccagcatctttgagtgcaagcctcagcgtgttcacaaactgattcacaacagtcatgacccaagttactttgcttgtctgattaaggaagacgctgtccaccggcagagtatctgctatgtgttcaaagccgatgatcaaacaaaagtgcctgagatcatcagctccatccgtcaggcggggaagatcgcccggcaggaggagctgcactgcccgtccgagttcgacgacacgttttccaagaagttcgaggtgctcttctgcggccgcgtgacggtggcgcacaagaaggctccgccggccctgatcgacgagtgcatcgagaagttcaatcacgtcagcggcagccgggggtccgagagcccccgccccaacccgccccatgccgcgcccacagggagccaggagcctgtgcgcaggcccatgcgcaagtccttctcccagcccggcctgcgctcgctggcctttaggaaggagctgcaggatgggggcctccgaagcagcggcttcttcagctccttcgaggagagcgacattgagaaccacctcattagcggacacaatattgtgcagcccacagatatcgaggaaaatcgaactatgctcttcacgattggccagtctgaagtttacctcatcagtcctgacaccaaaaaaatagcattggagaaaaattttaaggagatatccttttgctctcagggcatcagacacgtggaccactttgggtttatctgtcgggagtcttccggaggtggcggctttcattttgtctgttacgtgtttcagtgcacaaatgaggctctggttgatgaaattatgatgaccctgaaacaggccttcacggtggccgcagtgcagcagacagctaaggcgccagcccagctgtgtgagggctgccccctgcaaagcctgcacaagctctgtgagaggatagagggaatgaattcttccaaaacaaaactagaactgcaaaagcacctgacgacattaacaaatcaggagcaggcgactatttttgaagaggttcagaaattgagaccgagaaatgagcagcgagagaatgaattgattatttcttttctgagatgtttatatgaagagaaacagaaagaacacatccatattggggagatgaagcagacatcgcagatggcagcagagaatattggaagtgaattaccacccagtgccactcgatttaggctagatatgctgaaaaacaaagcaaagagatctttaacagagtctttagaaagtattttgtcccggggtaataaagccagaggcctgcaggaacactccatcagtgtggatctggatagctccctgtctagtacattaagtaacaccagcaaagagccatctgtgtgtgaaaaggaggccttgcccatctctgagagctcctttaagctcctcggctcctcggaggacctgtccagtgactcggagagtcatctcccagaagagccagctccgctgtcgccccagcaggccttcaggaggcgagcaaacaccctgagtcacttccccatcgaatgccaggaacctccacaacctgcccgggggtccccgggggtttcgcaaaggaaacttatgaggtatcactcagtgagcacagagacgcctcatgaacgaaaggactttgaatccaaagcaaaccatcttggtgattctggt
gggactcctgtgaagacccggaggcattcctggaggcagcagatattcct
ccgagtagccaccccgcagaaggcgtgcgattcttccagcagatatgaag
attattcagagctgggagagcttcccccacgatctcctttagaaccagtt
tgtgaagatgggccctttggccccccaccagaggaaaagaaaaggacatc
tcgtgagctccgagagctgtggcaaaaggctattcttcaacagatactgc
tgcttagaatggagaaggaaaatcagaagctccaagcctctgaaaatgat
ttgctgaacaagcgcctgaagctcgattatgaagaaattactccctgtct
taaagaagtaactacagtgtgggaaaagatgcttagcactccaggaagat
caaaaattaagtttgacatggaaaaaatgcactcggctgttgggcaaggt
gtgccacgtcatcaccgaggtgaaatctggaaatttctagctgagcaatt
ccaccttaaacaccagtttcccagcaaacagcagccaaaggatgtgccat
acaaagaactcttaaagcagctgacttcccagcagcatgcgattcttatt
gaccttgggcgaacctttcctacacacccatacttctctgcccagcttgg
agcaggacagctatcgctttacaacattttgaaggcctactcacttctag
accaggaagtgggatattgccaaggtctcagctttgtagcaggcattttg
cttcttcatatgagtgaggaagaggcgtttaaaatgctcaagtttctgat
gtttgacatggggctgcggaaacagtatcggccagacatgattattttac
agatccagatgtaccagctctcgaggttgcttcatgattaccacagagac
ctctacaatcacctggaggagcacgagatcggccccagcctctacgctgc
cccctggttcctcaccatgtttgcctcacagttcccgctgggattcgtag
ccagagtctttgatatgatttttcttcagggaacagaggtcatatttaaa
gtggctttaagtctgttgggaagccataagcccttgattctgcagcatga
aaacctagaaaccatagttgactttataaaaagcacgctacccaaccttg
gcttggtacagatggaaaagaccatcaatcaggtatttgaaatggacatc
gctaaacagttacaagcttatgaagttgagtaccacgtccttcaagaaga
acttatcgattcctctcctctcagtgacaaccaaagaatggataaattag
agaaaaccaacagcagcttacgcaaacagaaccttgacctccttgaacag
ttgcaggtggcaaatggtaggatccaaagccttgaggccaccattgagaa
gctcctgagcagtgagagcaagctgaagcaggccatgcttaccttagaac
tggagcggtcggccctgctgcagacggtggaggagctgcggcggcggagc
gcagagcccagcgaccgggagcctgagtgcacgcagcccgagcccacggg
cgactgagtcgac

Amino Acid Sequence

MDYKDDDDKMEPITFTARKHLLSNEVSVDFGLQLVGSLPVHSLTTMPMLP
WVVAEVRRLSRQSTRKEPVTKQVRLCVSPSGLRCEPEPGRSQQWDPLIYS
SIFECKPQRVHKLIHNSHDPSYFACLIKEDAVHRQSICYVFKADDQTKVP
EIISSIRQAGKIARQEELHCPSEFDDTFSKKFEVLFCGRVTVAHKKAPPA
LIDECIEKFNHVSGSRGSESPRPNPPHAAPTGSQEPVRRPMRKSFSQPGL
RSLAFRKELQDGGLRSSGFFSSFEESDIENHLISGHNIVQPTDIEENRTM
LFTIGQSEVYLISPDTKKIALEKNFKEISFCSQGIRHVDHFGFICRESSG
GGGFHFVCYVFQCTNEALVDEIMMTLKQAFTVAAVQQTAKAPAQLCEGCP
LQSLHKLCERIEGMNSSKTKLELQKHLTTLTNQEQATIFEEVQKLRPRNE
QRENELIISFLRCLYEEKQKEHIHIGEMKQTSQMAAENIGSELPPSATRF
RLDMLKNKAKRSLTESLESILSRGNKARGLQEHSISVDLDSSLSSTLSNT
SKEPSVCEKEALPISESSFKLLGSSEDLSSDSESHLPEEPAPLSPQQAFR
RRANTLSHFPIECQEPPQPARGSPGVSQRKLMRYHSVSTETPHERKDFES
KANHLGDSGGTPVKTRRHSWRQQIFLRVATPQKACDSSSRYEDYSELGEL
PPRSPLEPVCEDGPFGPPPEEKKRTSRELRELWQKAILQQILLLRMEKEN
QKLQASENDLLNKRLKLDYEEITPCLKEVTTVWEKMLSTPGRSKIKFDME
KMHSAVGQGVPRHHRGEIWKFLAEQFHLKHQFPSKQQPKDVPYKELLKQL
TSQQHAILIDLGRTFPTHPYFSAQLGAGQLSLYNILKAYSLLDQEVGYCQ
GLSFVAGILLLHMSEEEAFKMLKFLMFDMGLRKQYRPDMIILQIQMYQLS
RLLHDYHRDLYNHLEEHEIGPSLYAAPWFLTMFASQFPLGFVARVFDMIF
LQGTEVIFKVALSLLGSHKPLILQHENLETIVDFIKSTLPNLGLVQMEKT
INQVFEMDIAKQLQAYEVEYHVLQEELIDSSPLSDNQRMDKLEKTNSSLR
KQNLDLLEQLQVANGRIQSLEATIEKLLSSESKLKQAMLTLELERSALLQ
TVEELRRRSAEPSDREPECTQPEPTGD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11268
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence difference compared with NM_015173 CDS:; C1266a (silent in T422); ; Estimated MW of expressed protein: 134207 Daltons