cDNA Clone TBC1D1

• Plasmid: pCMV5-FLAG TBC1D1 M1-V700
• Parent Plasmid: pCMV5
• Expressed: FLAG-TBC1D1 M1-V700
• Genbank: NM_015173
• Tag: FLAG
• Species: Human

Sequence of Insert

ggtaccgccaccATGGACTACAAGGACGACGATGACAAGatggaaccaat
aacattcacagcaaggaaacatctgctttctaacgaggtctcggtggatt
ttggcctgcagctggtgggctccctgcctgtgcattccctgaccaccatg
cccatgctgccctgggttgtggctgaggtgcgaagactcagcaggcagtc
caccagaaaggaacctgtaaccaagcaagtccggctttgcgtttcaccct
ctggactgagatgtgaacctgagccagggagaagtcaacagtgggatccc
ctgatctattccagcatctttgagtgcaagcctcagcgtgttcacaaact
gattcacaacagtcatgacccaagttactttgcttgtctgattaaggaag
acgctgtccaccggcagagtatctgctatgtgttcaaagccgatgatcaa
acaaaagtgcctgagatcatcagctccatccgtcaggcggggaagatcgc
ccggcaggaggagctgcactgcccgtccgagttcgacgacacgttttcca
agaagttcgaggtgctcttctgcggccgcgtgacggtggcgcacaagaag
gctccgccggccctgatcgacgagtgcatcgagaagttcaatcacgtcag
cggcagccgggggtccgagagcccccgccccaacccgccccatgccgcgc
ccacagggagccaggagcctgtgcgcaggcccatgcgcaagtccttctcc
cagcccggcctgcgctcgctggcctttaggaaggagctgcaggatggggg
cctccgaagcagcggcttcttcagctccttcgaggagagcgacattgaga
accacctcattagcggacacaatattgtgcagcccacagatatcgaggaa
aatcgaactatgctcttcacgattggccagtctgaagtttacctcatcag
tcctgacaccaaaaaaatagcattggagaaaaattttaaggagatatcct
tttgctctcagggcatcagacacgtggaccactttgggtttatctgtcgg
gagtcttccggaggtggcggctttcattttgtctgttacgtgtttcagtg
cacaaatgaggctctggttgatgaaattatgatgaccctgaaacaggcct
tcacggtggccgcagtgcagcagacagctaaggcgccagcccagctgtgt
gagggctgccccctgcaaagcctgcacaagctctgtgagaggatagaggg
aatgaattcttccaaaacaaaactagaactgcaaaagcacctgacgacat
taacaaatcaggagcaggcgactatttttgaagaggttcagaaattgaga
ccgagaaatgagcagcgagagaatgaattgattatttcttttctgagatg
tttatatgaagagaaacagaaagaacacatccatattggggagatgaagc
agacatcgcagatggcagcagagaatattggaagtgaattaccacccagt
gccactcgatttaggctagatatgctgaaaaacaaagcaaagagatcttt
aacagagtctttagaaagtattttgtcccggggtaataaagccagaggcc
tgcaggaacactccatcagtgtggatctggatagctccctgtctagtaca
ttaagtaacaccagcaaagagccatctgtgtgtgaaaaggaggccttgcc
catctctgagagctcctttaagctcctcggctcctcggaggacctgtcca
gtgactcggagagtcatctcccagaagagccagctccgctgtcgccccag
caggccttcaggaggcgagcaaacaccctgagtcacttccccatcgaatg
ccaggaacctccacaacctgcccgggggtccccgggggtttcgcaaagga
aacttatgaggtatcactcagtgagcacagagacgcctcatgaacgaaag
gactttgaatccaaagcaaaccatcttggtgattctggtgggactcctgt
gaagacccggaggcattcctggaggcagcagatattcctccgagtagcca
ccccgcagaaggcgtgcgattcttccagcagatatgaagattattcagag
ctgggagagcttcccccacgatctcctttagaaccagtttgagtcgac

Amino Acid Sequence

MDYKDDDDKMEPITFTARKHLLSNEVSVDFGLQLVGSLPVHSLTTMPMLP
WVVAEVRRLSRQSTRKEPVTKQVRLCVSPSGLRCEPEPGRSQQWDPLIYS
SIFECKPQRVHKLIHNSHDPSYFACLIKEDAVHRQSICYVFKADDQTKVP
EIISSIRQAGKIARQEELHCPSEFDDTFSKKFEVLFCGRVTVAHKKAPPA
LIDECIEKFNHVSGSRGSESPRPNPPHAAPTGSQEPVRRPMRKSFSQPGL
RSLAFRKELQDGGLRSSGFFSSFEESDIENHLISGHNIVQPTDIEENRTM
LFTIGQSEVYLISPDTKKIALEKNFKEISFCSQGIRHVDHFGFICRESSG
GGGFHFVCYVFQCTNEALVDEIMMTLKQAFTVAAVQQTAKAPAQLCEGCP
LQSLHKLCERIEGMNSSKTKLELQKHLTTLTNQEQATIFEEVQKLRPRNE
QRENELIISFLRCLYEEKQKEHIHIGEMKQTSQMAAENIGSELPPSATRF
RLDMLKNKAKRSLTESLESILSRGNKARGLQEHSISVDLDSSLSSTLSNT
SKEPSVCEKEALPISESSFKLLGSSEDLSSDSESHLPEEPAPLSPQQAFR
RRANTLSHFPIECQEPPQPARGSPGVSQRKLMRYHSVSTETPHERKDFES
KANHLGDSGGTPVKTRRHSWRQQIFLRVATPQKACDSSSRYEDYSELGEL
PPRSPLEPV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11263
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence difference compared with NM_015173 CDS:; C1266a (silent in T422); ; Estimated MW of expressed protein : 79935 Daltons