cDNA Clone ESC4 (yeast)

• Synonyms: RTTFL
• Plasmid: pRS415 13Myc RTT-FL 255/361/390/502A
• Parent Plasmid: pRS415
• Tag: 13Myc
• Species: Yeast

Amino Acid Sequence

MAGRRIPGLINGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLN
GEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDL
NGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEED
LNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLINHTMGTSLLFEQLNF
LILVAAEAELPIAHSTRKLLMDNSCNNCQIYELYNENLKDVKTDKDWFMN
KFGPQTVHFVISNTINFPFYKIVYFDLLIPVVSHTWVQDSVKTKRHLRTN
MYSPNPFHLLRDCQVYISKSSFNKCEYILYSDLLHLLGGTLVNYISNRTT
HVIVQSPQDPIIATVSKLTFGSFSSSSTNKHTEKPLREWKFVYPIWILYH
FKMAKPLKGELATLCELDMQDTSEEQLFAKWEEVIGDKQTSSAQLTLHPN
KTLFKNHHFAISPDLNFFTPLYWFLKGFIEDLDGKVTPLSFSDDLKSVYQ
AFPDIDCYIGHSANSPILEKTKSIKPEIHVGNVSWLFYMFALQKFTPVAQ
CKLIHQPFHAKLFTSKELTVAYTNYFGAQRFYIQRLVEILGGLSTPELTR
KNTHLITKSTIGKKFKVAKKWSLDPQNAIIVTNHMWLEQCYMNNSKLNPK
DSRFQNFKLDDNMGWNIGQIGMDHSSLPTPKNLSMVTYDAQSISEKPPPT
NDQMDQINDNTNVLSKKDGTPISSFENSIDEKIDKLQKISGEVAVTHSGD
LERSFVSRPSRASFPVVDSKKSNLQKKDSNSDISMETEVFCEGHEKREEK
EFTKPITEYDAPKKQEIREQSRKKNDIDYKKEEEETELQVQLGQRTKREI
KTSKKNEKEKETNECHIEVDQMTNEKQGEESTGKLISTEDVTSKKDTDKF
SHLFEGLSDNDDHINDEKPAVNSKYTTPKTSQNITSGVDTPTTAQTQVFM
PSSGNSRLAKTQAAKRLHTDIESLNEFQKNFKRKRIDSEEISLSQDVERS
NNNKELATKAEKILARFNELPNYDLKAVCTGCFHDGFNEVDIEILNQLGI
KIFDNIKETDKLNCIFAPKILRTEKFLKSLSFEPLKFALKPEFIIDLLKQ
IHSKKDKLSQININLFDYEINGINESIISKTKLPTKVFERANIRCINLVN
DIPGGVDTIGSVLKAHGIEKINVLRSKKCTFEDIIPNDVSKQENGGIFKY
VLIVTKASQVKKFTKLINDRDKNETILIVEWNWCVESIFHLNVDFTSKKN
VLYQKKNN*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11261
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast non-intergrating expression plasmid.; Sequence differences (other than intended mutations) in ESC4 compared with reference CDS:; a4g (S2G) (resulting from generation of Nco1 site); t3006g (silent in S1002)