cDNA Clone ESC4 (yeast)

• Synonyms: RTTFL
• Plasmid: pRS415 RTT-FL 255/361/390/502A (ATG=Nco1)
• Parent Plasmid: pRS415
• Species: Yeast

Sequence of Insert

GGATCCTCTCCAATAAGATATCCCAATAAGCTATTGAGAATTTCAAGCGCGTGAAATTCAAATTTATCATCAGAGAACGATGTGCGGGAACTCCAGGGCTTTTCTGTATTTTCTCATAGACATTATTTATCAGTAATTGCAGCTGTGTAACAATAAAATTGACGCCACTTTCTTCGTCTTGTAGCTCCTCGTTAAGCTGCGAAA
TTAACCTGCTCGTATTCACAGTAACACACTTAACCACAGAATGTTCTTCG
ACATTAGAAATCTCTTGTATTTTTTGTACATCCCAAAAGAATTCAGACAT
TTCTATTGAACCATTCCAGAAATGTTGTTCCAGAAGTTTATTCTCTTGAA
CCTTTACACTATTTCGTATTTTCTCGGTTTTAGCCGCCCACTTTTCACGC
GTTAACAGGGTAAGCTATATCAGATGTGACTTTTCTTTTTCACTCATCTA
ATGTTGTTTTTCGCGTCACATTTTTTGCCAATAAATAAATACATCACAAT
GTTCTATATTGTATATTCAATGGAAGTCATTAGGTTATTGGGCAAGATTT
CAAGGAGGTAGCATACTGGTAAATATACGATCCATGGGCACATCACTTCT
GTTTGAGCAGCTAAATTTTTTAATTTTAGTGGCAGCAGAGGCTGAGCTTC
CCATCGCTCACTCTACTAGAAAGCTTTTGATGGATAATTCATGCAATAAC
TGTCAAATATACGAGCTTTATAACGAAAATTTGAAAGATGTCAAGACGGA
TAAAGATTGGTTCATGAACAAATTCGGCCCGCAAACAGTACATTTTGTCA
TTAGTAATACAATCAACTTTCCTTTTTATAAGATAGTTTATTTCGATCTT
TTGATCCCGGTGGTATCACACACTTGGGTCCAGGATTCGGTCAAGACGAA
GAGACATTTGAGGACAAATATGTATTCACCTAACCCATTTCATTTGCTCC
GAGACTGTCAAGTATATATTTCCAAAAGCTCGTTTAATAAGTGTGAATAC
ATATTATACTCTGATTTGCTTCATTTATTAGGAGGCACTTTAGTCAATTA
CATCTCAAATAGAACGACGCATGTGATTGTTCAAAGTCCCCAGGACCCGA
TCATAGCAACGGTCAGTAAATTGACATTTGGCTCTTTTTCCTCTTCATCG
ACCAACAAGCACACCGAAAAGCCTTTGAGAGAGTGGAAATTTGTCTATCC
AATATGGATATTATATCATTTCAAAATGGCGAAGCCCTTGAAGGGCGAGC
TTGCTACTTTGTGTGAGCTTGATATGCAGGACACCAGTGAGGAACAGTTA
TTCGCAAAATGGGAAGAGGTTATTGGCGATAAGCAGACATCATCAGCGCA
GCTTACTCTTCATCCAAACAAAACTTTATTCAAAAATCACCACTTTGCTA
TCTCACCAGATTTAAACTTTTTCACACCTTTGTATTGGTTTTTGAAAGGA
TTTATCGAGGATTTGGACGGCAAAGTAACTCCGTTGAGTTTTTCTGACGA
TCTGAAGTCTGTTTACCAAGCTTTTCCTGATATTGACTGTTATATCGGCC
ACTCTGCTAACTCACCGATACTAGAAAAAACCAAAAGCATTAAACCGGAA
ATTCATGTTGGGAACGTAAGTTGGTTGTTTTATATGTTTGCGCTGCAAAA
ATTTACACCTGTAGCGCAATGTAAACTTATTCACCAACCATTTCATGCGA
AATTATTTACTTCTAAGGAATTAACAGTCGCGTACACTAATTATTTCGGC
GCCCAAAGGTTTTATATTCAGAGGTTAGTAGAAATATTAGGAGGACTTAG
CACACCTGAACTAACAAGAAAAAACACACACTTGATCACGAAGAGTACAA
TTGGCAAAAAATTCAAAGTCGCAAAAAAATGGTCACTGGACCCGCAAAAT
GCCATTATTGTGACAAATCATATGTGGTTAGAGCAATGTTATATGAATAA
TTCCAAGTTAAACCCCAAAGACAGTAGATTTCAAAATTTTAAATTGGATG
ATAACATGGGATGGAATATCGGCCAAATAGGAATGGATCATTCATCTCTC
CCAACTCCAAAAAACCTATCGATGGTGACTTACGACGCGCAATCCATATC
AGAGAAGCCGCCTCCCACAAATGATCAAATGGATCAAATTAACGATAACA
CCAATGTATTATCAAAGAAAGATGGTACGCCCATTAGTAGTTTTGAGAAC
TCAATTGATGAAAAGATTGACAAATTACAAAAAATATCAGGAGAGGTGGC
TGTAACCCATTCTGGAGATTTAGAACGGAGTTTTGTTTCCAGACCTTCAA
GGGCAAGTTTTCCAGTAGTTGACAGTAAAAAATCTAATTTACAAAAGAAA
GACTCAAACTCAGATATTAGTATGGAAACAGAGGTTTTCTGTGAAGGACA
TGAAAAAAGAGAAGAAAAGGAGTTTACGAAACCTATTACGGAGTATGATG
CACCTAAGAAACAAGAAATAAGGGAACAGTCACGCAAGAAGAATGACATA
GATTATAAAAAAGAAGAGGAAGAAACGGAGCTGCAAGTTCAGCTAGGACA
ACGAACTAAACGAGAGATCAAAACTTCGAAGAAAAATGAAAAGGAGAAAG
AAACCAATGAATGTCACATTGAAGTAGATCAAATGACGAATGAAAAGCAA
GGGGAGGAAAGTACAGGAAAACTGATTTCTACGGAAGACGTCACTTCTAA
AAAAGATACAGATAAATTCTCGCATTTGTTTGAAGGATTATCTGATAATG
ATGATCACATTAATGACGAAAAACCGGCAGTTAATTCCAAATATACCACA
CCCAAGACGTCTCAAAATATTACGAGTGGCGTTGATACTCCAACCACCGC
GCAAACGCAAGTTTTTATGCCATCAAGTGGCAATTCAAGGTTAGCAAAGA
CACAAGCTGCGAAGAGATTGCATACAGATATTGAATCATTAAATGAATTT
CAGAAAAACTTTAAAAGGAAGAGAATTGATAGCGAGGAGATTTCATTGTC
CCAAGATGTTGAAAGGTCAAATAATAACAAAGAACTGGCTACTAAAGCGG
AAAAGATATTAGCTAGATTTAATGAACTACCAAATTATGACCTAAAAGCG
GTTTGCACCGGTTGTTTCCATGATGGATTTAATGAGGTTGACATCGAAAT
ATTGAACCAACTAGGTATAAAAATTTTTGACAATATTAAAGAAACGGATA
AATTGAATTGTATTTTTGCTCCAAAGATTTTGAGAACCGAGAAATTTTTA
AAAAGCTTAAGTTTTGAACCGTTAAAATTCGCATTGAAACCTGAATTTAT
TATTGATTTACTGAAACAAATTCATTCTAAAAAAGATAAACTTTCTCAAA
TAAATATAAACTTGTTTGATTATGAGATAAATGGAATTAATGAGAGTATA
ATATCAAAAACCAAACTACCTACTAAGGTGTTCGAAAGAGCGAATATTAG
ATGCATTAACTTAGTCAATGATATACCGGGTGGTGTAGATACAATAGGCT
CTGTTCTAAAGGCACATGGTATCGAAAAAATCAACGTTTTACGTAGTAAG
AAATGCACATTTGAAGACATAATCCCAAATGACGTTTCTAAGCAGGAAAA
CGGAGGCATTTTTAAATACGTACTTATCGTCACCAAAGCTTCACAGGTTA
AAAAATTTACCAAACTGATTAATGATCGTGATAAAAACGAAACAATTTTG
ATTGTTGAATGGAATTGGTGTGTTGAATCAATTTTTCATCTCAATGTTGA
TTTTACATCAAAGAAAAATGTTTTGTATCAAAAGAAAAATAACTAAGTCGAC

Amino Acid Sequence

MGTSLLFEQLNFLILVAAEAELPIAHSTRKLLMDNSCNNCQIYELYNENL
KDVKTDKDWFMNKFGPQTVHFVISNTINFPFYKIVYFDLLIPVVSHTWVQ
DSVKTKRHLRTNMYSPNPFHLLRDCQVYISKSSFNKCEYILYSDLLHLLG
GTLVNYISNRTTHVIVQSPQDPIIATVSKLTFGSFSSSSTNKHTEKPLRE
WKFVYPIWILYHFKMAKPLKGELATLCELDMQDTSEEQLFAKWEEVIGDK
QTSSAQLTLHPNKTLFKNHHFAISPDLNFFTPLYWFLKGFIEDLDGKVTP
LSFSDDLKSVYQAFPDIDCYIGHSANSPILEKTKSIKPEIHVGNVSWLFY
MFALQKFTPVAQCKLIHQPFHAKLFTSKELTVAYTNYFGAQRFYIQRLVE
ILGGLSTPELTRKNTHLITKSTIGKKFKVAKKWSLDPQNAIIVTNHMWLE
QCYMNNSKLNPKDSRFQNFKLDDNMGWNIGQIGMDHSSLPTPKNLSMVTY
DAQSISEKPPPTNDQMDQINDNTNVLSKKDGTPISSFENSIDEKIDKLQK
ISGEVAVTHSGDLERSFVSRPSRASFPVVDSKKSNLQKKDSNSDISMETE
VFCEGHEKREEKEFTKPITEYDAPKKQEIREQSRKKNDIDYKKEEEETEL
QVQLGQRTKREIKTSKKNEKEKETNECHIEVDQMTNEKQGEESTGKLIST
EDVTSKKDTDKFSHLFEGLSDNDDHINDEKPAVNSKYTTPKTSQNITSGV
DTPTTAQTQVFMPSSGNSRLAKTQAAKRLHTDIESLNEFQKNFKRKRIDS
EEISLSQDVERSNNNKELATKAEKILARFNELPNYDLKAVCTGCFHDGFN
EVDIEILNQLGIKIFDNIKETDKLNCIFAPKILRTEKFLKSLSFEPLKFA
LKPEFIIDLLKQIHSKKDKLSQININLFDYEINGINESIISKTKLPTKVF
ERANIRCINLVNDIPGGVDTIGSVLKAHGIEKINVLRSKKCTFEDIIPND
VSKQENGGIFKYVLIVTKASQVKKFTKLINDRDKNETILIVEWNWCVESI
FHLNVDFTSKKNVLYQKKNN*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11254
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast non-intergrating expression plasmid.; Sequence differences (other than intended mutations) in ESC4 compared with reference CDS:; a4g (S2G) (resulting from generation of Nco1 site); t3006g (silent in S1002)