cDNA Clone AS160

• Synonyms: TBC1D4
• Plasmid: pCMV5-FLAG AS160(B) M1-I762
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG-AS160(B) M1-I762
• Genbank: NM_014832
• Tag: FLAG
• Species: Human

Sequence of Insert

ggatccATGGAGCCGCCCAGCTGCATTCAGGATGAGCCGTTCCCGCACCCCCTGGAGCCCGAGCCGGGCGTCTCAGCTCAGCCCGGCCCCGGGAAGCCAAGCGATAAGCGGTTCCGGCTGTGGTACGTTGGGGGGTCGTGCCTGGACCACAGGACCACGCTGCCTATGCTGCCCTGGCTCATGGCCGAGATCCGCAGGCGCAGCCAGAAGCCCGAGGCGGGCGGCTGCGGGGCGCCGGCGGCCCGAGAGGTGATCCTGGTGCTCAGCGCGCCCTTCCTGCGTTGCGTCCCCGCGCCGGGCGCTGGG
GCCTCGGGGGGCACTAGTCCGTCGGCCACGCAGCCCAACCCGGCGGTATT
CATCTTCGAGCACAAGGCGCAGCATATCTCGCGCTTCATCCACAACAGCC
ACGACCTCACCTACTTTGCCTACCTGATCAAGGCGCAGCCCGACGACCCC
GAGTCGCAGATGGCCTGCCACGTTTTCCGCGCCACAGACCCCAGCCAGGT
TCCTGATGTTATTAGCAGCATAAGGCAATTATCTAAAGCGGCCATGAAAG
AGGATGCCAAACCCAGCAAAGATAATGAGGACGCCTTTTACAACTCTCAG
AAGTTCGAAGTCCTGTACTGTGGAAAGGTGACCGTGACCCACAAGAAGGC
CCCCTCAAGCCTCATCGATGACTGCATGGAGAAGTTCAGCCTGCACGAAC
AGCAGCGCCTGAAGATCCAAGGCGAGCAGCGCGGTCCGGACCCAGGAGAG
GACCTGGCTGACTTGGAGGTGGTGGTGCCCGGGTCCCCCGGAGACTGCCT
GCCGGAGGAGGCTGACGGCACCGACACCCACCTTGGCTTACCTGCCGGGG
CCAGCCAGCCTGCCCTGACCAGCTCTCGGGTCTGCTTCCCTGAGCGGATT
TTGGAAGATTCTGGCTTTGATGAGCAGCAGGAGTTTCGGTCTCGGTGCAG
CAGTGTCACCGGCGTGCAACGGAGAGTTCACGAGGGCAGCCAGAAATCCC
AGCCGCGACGGAGACACGCGAGCGCACCCAGTCACGTCCAGCCCTCGGAC
TCGGAGAAGAACAGGACCATGCTCTTCCAGGTTGGGCGATTTGAGATTAA
CCTTATCAGTCCAGACACTAAATCAGTTGTGCTAGAAAAGAATTTTAAAG
ATATCTCCCCTTGTTCTCAGGGTATAAAGCATGTGGATCACTTTGGCTTT
ATCTGCCGGGAGTCTCCAGAGCCTGGACTTAGCCAGTATATTTGTTATGT
ATTCCAGTGTGCCAGCGAATCTCTGGTTGATGAGGTAATGCTGACTCTGA
AACAGGCCTTCAGTACGGCGGCTGCCCTGCAGAGTGCCAAGACGCAGATT
AAACTGTGTGAGGCCTGCCCGATGCACTCTTTGCATAAGCTCTGTGAAAG
GATTGAAGGTCTCTACCCACCAAGAGCCAAGCTGGTGATACAGAGGCATC
TCTCATCACTGACAGATAATGAGCAAGCTGACATCTTTGAAAGAGTTCAG
AAAATGAAGCCAGTCAGTGACCAGGAAGAAAATGAACTTGTGATTTTACA
CCTGAGGCAGCTGTGTGAAGCCAAGCAGAAGACACACGTGCACATCGGGG
AAGGCCCTGCTACTATTTCAAATAGTACAATCCCAGAAAATGCAACAAGC
AGTGGAAGGTTCAAACTTGACATTCTGAAAAATAAAGCTAAGAGATCCTT
AACTAGCTCCCTGGAAAATATCTTCTCAAGGGGAGCTAACAGAATGAGAG
GTCGGCTTGGAAGTGTGGACAGTTTTGAACGGTCCAACAGTCTTGCTTCA
GAGAAGGACTACTCACCAGGGGATTCTCCACCAGGGACACCGCCAGCGTC
CCCACCGTCCTCAGCTTGGCAAACGTTTCCCGAAGAGGATTCCGACTCCC
CGCAGTTTCGAAGACGGGCACACACGTTCAGCCACCCACCTTCAAGCACA
AAGAGAAAGCTGAATTTGCAGGATGGGAGGGCTCAGGGTGTGCGTTCCCC
TCTGCTGAGGCAGAGCTCCAGTGAACAGTGCAGCAATCTTTCGTCAGTTC
GACGCATGTACAAGGAGAGTAATTCTTCCTCCAGTCTTCCAAGTCTTCAC
ACTTCCTTCTCTGCCCCTTCCTTCACTGCCCCCTCTTTCCTGAAAAGCTT
TTACCAGAATTCAGGTAGACTGTCCCCACAGTATGAAAATGAAATCAGTG
ATGGAGAAGGGAGAAAAAGGACCTCATCTACCTGCAGCAATGAGTCCCTA
AGTGTGGGAGGAACCTCTGTCACTCCTCGCCGGATCtaagcggccgc

Amino Acid Sequence

MDYKDDDDKGSMEPPSCIQDEPFPHPLEPEPGVSAQPGPGKPSDKRFRLW
YVGGSCLDHRTTLPMLPWLMAEIRRRSQKPEAGGCGAPAAREVILVLSAP
FLRCVPAPGAGASGGTSPSATQPNPAVFIFEHKAQHISRFIHNSHDLTYF
AYLIKAQPDDPESQMACHVFRATDPSQVPDVISSIRQLSKAAMKEDAKPS
KDNEDAFYNSQKFEVLYCGKVTVTHKKAPSSLIDDCMEKFSLHEQQRLKI
QGEQRGPDPGEDLADLEVVVPGSPGDCLPEEADGTDTHLGLPAGASQPAL
TSSRVCFPERILEDSGFDEQQEFRSRCSSVTGVQRRVHEGSQKSQPRRRH
ASAPSHVQPSDSEKNRTMLFQVGRFEINLISPDTKSVVLEKNFKDISPCS
QGIKHVDHFGFICRESPEPGLSQYICYVFQCASESLVDEVMLTLKQAFST
AAALQSAKTQIKLCEACPMHSLHKLCERIEGLYPPRAKLVIQRHLSSLTD
NEQADIFERVQKMKPVSDQEENELVILHLRQLCEAKQKTHVHIGEGPATI
SNSTIPENATSSGRFKLDILKNKAKRSLTSSLENIFSRGANRMRGRLGSV
DSFERSNSLASEKDYSPGDSPPGTPPASPPSSAWQTFPEEDSDSPQFRRR
AHTFSHPPSSTKRKLNLQDGRAQGVRSPLLRQSSSEQCSNLSSVRRMYKE
SNSSSSLPSLHTSFSAPSFTAPSFLKSFYQNSGRLSPQYENEISDGEGRK
RTSSTCSNESLSVGGTSVTPRRI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU11239
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence differences in cloned AS160 (B) (long form) compared with NM_014832: ; S387P, S537A, (K958R), delta R733-S740, (delta G865). These differences are present in all AS160 (long form) DU clones.; ; Estimated MW of expressed protein : 85274 Daltons