cDNA Clone CDC45L

• Plasmid: pGEX6P-3-CDC45L
• Parent Plasmid: pGEX6P3
• Expressed: GST-CDC45L
• Genbank: NM_003504
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTTCGTGTCCGATTTCCGCAAAGAGTTCTACGAGGTGGTCCAGAGCCAGAGGGTCCTTCTCTTCGTGGCCTCGGACGTGGATGCTCTGTGTGCGTGCAAGATCCTTCAGGCCTTGTTCCAGTGTGACCACGTGCAATATACGCTGGTTCCAGTTTCTGGGTGGCAAGAACTTGAAACTGCATTTCTTGAGCATAAAGAACAGTTTCATTATTTTATTCTCATAAACTGTGGAGCTAATGTAGACCTATTGGATATTCTTCAACCTGATGAAGACACTATATTCTTTGTGTGTGACACC
CATAGGCCAGTCAATGTCGTCAATGTATACAACGATACCCAGATCAAATT
ACTCATTAAACAAGATGATGACCTTGAAGTTCCCGCCTATGAAGACATCT
TCAGGGATGAAGAGGAGGATGAAGAGCATTCAGGAAATGACAGTGATGGG
TCAGAGCCTTCTGAGAAGCGCACACGGTTAGAAGAGGAGATAGTGGAGCA
AACCATGCGGAGGAGGCAGCGGCGAGAGTGGGAGGCCCGGAGAAGAGACA
TCCTCTTTGACTACGAGCAGTATGAATATCATGGGACATCGTCAGCCATG
GTGATGTTTGAGCTGGCTTGGATGCTGTCCAAGGACCTGAATGACATGCT
GTGGTGGGCCATCGTTGGACTAACAGACCAGTGGGTGCAAGACAAGATCA
CTCAAATGAAATACGTGACTGATGTTGGTGTCCTGCAGCGCCACGTTTCC
CGCCACAACCACCGGAACGAGGATGAGGAGAACACACTCTCCGTGGACTG
CACACGGATCTCCTTTGAGTATGACCTCCGCCTGGTGCTCTACCAGCACT
GGTCCCTCCATGACAGCCTGTGCAACACCAGCTATACCGCAGCCAGGTTC
AAGCTGTGGTCTGTGCATGGACAGAAGCGGCTCCAGGAGTTCCTTGCAGA
CATGGGTCTTCCCCTGAAGCAGGTGAAGCAGAAGTTCCAGGCCATGGACA
TCTCCTTGAAGGAGAATTTGCGGGAAATGATTGAAGAGTCTGCAAATAAA
TTTGGGATGAAGGACATGCGCGTGCAGACTTTCAGCATTCATTTTGGGTT
CAAGCACAAGTTTCTGGCCAGCGACGTGGTCTTTGCCACCATGTCTTTGA
TGGAGAGCCCCGAGAAGGATGGCTCAGGGACAGATCACTTCATCCAGGCT
CTGGACAGCCTCTCCAGGAGTAACCTGGACAAGCTGTACCATGGCCTGGA
ACTCGCCAAGAAGCAGCTGCGAGCCACCCAGCAGACCATTGCCAGCTGCC
TTTGCACCAACCTCGTCATCTCCCAGGGGCCTTTCCTGTACTGCTCTCTC
ATGGAGGGCACTCCAGATGTCATGCTGTTCTCTAGGCCGGCATCCCTAAG
CCTGCTCAGCAAACACCTGCTCAAGTCCTTTGTGTGTTCGACAAAGAACC
GGCGCTGCAAACTGCTGCCCCTGGTGATGGCTGCCCCCCTGAGCATGGAG
CATGGCACAGTGACCGTGGTGGGCATCCCCCCAGAGACCGACAGCTCGGA
CAGGAAGAACTTTTTTGGGAGGGCGTTTGAGAAGGCAGCGGAAAGCACCA
GCTCCCGGATGCTGCACAACCATTTTGACCTCTCAGTAATTGAGCTGAAA
GCTGAGGATCGGAGCAAGTTTCTGGACGCACTTATTTCCCTCCTGTCCTA
GGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMFVSDFRKEFYEVVQSQRV
LLFVASDVDALCACKILQALFQCDHVQYTLVPVSGWQELETAFLEHKEQF
HYFILINCGANVDLLDILQPDEDTIFFVCDTHRPVNVVNVYNDTQIKLLI
KQDDDLEVPAYEDIFRDEEEDEEHSGNDSDGSEPSEKRTRLEEEIVEQTM
RRRQRREWEARRRDILFDYEQYEYHGTSSAMVMFELAWMLSKDLNDMLWW
AIVGLTDQWVQDKITQMKYVTDVGVLQRHVSRHNHRNEDEENTLSVDCTR
ISFEYDLRLVLYQHWSLHDSLCNTSYTAARFKLWSVHGQKRLQEFLADMG
LPLKQVKQKFQAMDISLKENLREMIEESANKFGMKDMRVQTFSIHFGFKH
KFLASDVVFATMSLMESPEKDGSGTDHFIQALDSLSRSNLDKLYHGLELA
KKQLRATQQTIASCLCTNLVISQGPFLYCSLMEGTPDVMLFSRPASLSLL
SKHLLKSFVCSTKNRRCKLLPLVMAAPLSMEHGTVTVVGIPPETDSSDRK
NFFGRAFEKAAESTSSRMLHNHFDLSVIELKAEDRSKFLDALISLLS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU11227
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 92390 Daltons