cDNA Clone ESC4 (yeast)

• Synonyms: RTTFL
• Plasmid: pRS405 RTT-FL 13Myc
• Parent Plasmid: pRS405
• Genbank: NP_012024
• Tag: 13Myc
• Species: Yeast

Sequence of Insert

GGATCCTCTCCAATAAGATATCCCAATAAGCTATTGAGAATTTCAAGCGC
GTGAAATTCAAATTTATCATCAGAGAACGATGTGCGGGAACTCCAGGGCT
TTTCTGTATTTTCTCATAGACATTATTTATCAGTAATTGCAGCTGTGTAA
CAATAAAATTGACGCCACTTTCTTCGTCTTGTAGCTCCTCGTTAAGCTGC
GAAATTAACCTGCTCGTATTCACAGTAACACACTTAACCACAGAATGTTC
TTCGACATTAGAAATCTCTTGTATTTTTTGTACATCCCAAAAGAATTCAG
ACATTTCTATTGAACCATTCCAGAAATGTTGTTCCAGAAGTTTATTCTCT
TGAACCTTTACACTATTTCGTATTTTCTCGGTTTTAGCCGCCCACTTTTC
ACGCGTTAACAGGGTAAGCTATATCAGATGTGACTTTTCTTTTTCACTCA
TCTAATGTTGTTTTTCGCGTCACATTTTTTGCCAATAAATAAATACATCA
CAATGTTCTATATTGTATATTCAATGGAAGTCATTAGGTTATTGGGCAAG
ATTTCAAGGAGGTAGCATACTGGTAAATATACGATCCATGGCAGGTCGAC
GGATCCCCGGGTTAATTAACGGTGAACAAAAGCTAATCTCCGAGGAAGAC
TTGAACGGTGAACAAAAATTAATCTCAGAAGAAGACTTGAACGGACTCGA
CGGTGAACAAAAGTTGATTTCTGAAGAAGATTTGAACGGTGAACAAAAGC
TAATCTCCGAGGAAGACTTGAACGGTGAACAAAAATTAATCTCAGAAGAA
GACTTGAACGGACTCGACGGTGAACAAAAGTTGATTTCTGAAGAAGATTT
GAACGGTGAACAAAAGCTAATCTCCGAGGAAGACTTGAACGGTGAACAAA
AATTAATCTCAGAAGAAGACTTGAACGGACTCGACGGTGAACAAAAGTTG
ATTTCTGAAGAAGATTTGAACGGTGAACAAAAGCTAATCTCCGAGGAAGA
CTTGAACGGTGAACAAAAATTAATCTCAGAAGAAGACTTGAACGGACTCG
ACGGTGAACAAAAGTTGATTTCTGAAGAAGATTTGAACGGTGAACAAAAG
CTAATCTCCGAGGAAGACTTGAACGGTGAACAAAAATTAATCAATCACAC
CATGGGCACATCACTTCTGTTTGAGCAGCTAAATTTTTTAATTTTAGTGG
CAGCAGAGGCTGAGCTTCCCATCGCTCACTCTACTAGAAAGCTTTTGATG
GATAATTCATGCAATAACTGTCAAATATACGAGCTTTATAACGAAAATTT
GAAAGATGTCAAGACGGATAAAGATTGGTTCATGAACAAATTCGGCCCGC
AAACAGTACATTTTGTCATTAGTAATACAATCAACTTTCCTTTTTATAAG
ATAGTTTATTTCGATCTTTTGATCCCGGTGGTATCACACACTTGGGTCCA
GGATTCGGTCAAGACGAAGAGACATTTGAGGACAAATATGTATTCACCTA
ACCCATTTCATTTGCTCCGAGACTGTCAAGTATATATTTCCAAAAGCTCG
TTTAATAAGTGTGAATACATATTATACTCTGATTTGCTTCATTTATTAGG
AGGCACTTTAGTCAATTACATCTCAAATAGAACGACGCATGTGATTGTTC
AAAGTCCCCAGGACCCGATCATAGCAACGGTCAGTAAATTGACATTTGGC
TCTTTTTCCTCTTCATCGACCAACAAGCACACCGAAAAGCCTTTGAGAGA
GTGGAAATTTGTCTATCCAATATGGATATTATATCATTTCAAAATGGCGA
AGCCCTTGAAGGGCGAGCTTGCTACTTTGTGTGAGCTTGATATGCAGGAC
ACCAGTGAGGAACAGTTATTCGCAAAATGGGAAGAGGTTATTGGCGATAA
GCAGACATCATCATCGCAGCTTACTCTTCATCCAAACAAAACTTTATTCA
AAAATCACCACTTTGCTATCTCACCAGATTTAAACTTTTTCACACCTTTG
TATTGGTTTTTGAAAGGATTTATCGAGGATTTGGACGGCAAAGTAACTCC
GTTGAGTTTTTCTGACGATCTGAAGTCTGTTTACCAAGCTTTTCCTGATA
TTGACTGTTATATCGGCCACTCTGCTAACTCACCGATACTAGAAAAAACC
AAAAGCATTAAACCGGAAATTCATGTTGGGAACGTAAGTTGGTTGTTTTA
TATGTTTGCGCTGCAAAAATTTACACCTGTATCGCAATGTAAACTTATTC
ACCAACCATTTCATGCGAAATTATTTACTTCTAAGGAATTAACAGTCGCG
TACACTAATTATTTCGGCTCCCAAAGGTTTTATATTCAGAGGTTAGTAGA
AATATTAGGAGGACTTAGCACACCTGAACTAACAAGAAAAAACACACACT
TGATCACGAAGAGTACAATTGGCAAAAAATTCAAAGTCGCAAAAAAATGG
TCACTGGACCCGCAAAATGCCATTATTGTGACAAATCATATGTGGTTAGA
GCAATGTTATATGAATAATTCCAAGTTAAACCCCAAAGACAGTAGATTTC
AAAATTTTAAATTGGATGATAACATGGGATGGAATATCGGCCAAATAGGA
ATGGATCATTCATCTCTCCCAACTCCAAAAAACCTATCGATGGTGACTTA
CGACACGCAATCCATATCAGAGAAGCCGCCTCCCACAAATGATCAAATGG
ATCAAATTAACGATAACACCAATGTATTATCAAAGAAAGATGGTACGCCC
ATTAGTAGTTTTGAGAACTCAATTGATGAAAAGATTGACAAATTACAAAA
AATATCAGGAGAGGTGGCTGTAACCCATTCTGGAGATTTAGAACGGAGTT
TTGTTTCCAGACCTTCAAGGGCAAGTTTTCCAGTAGTTGACAGTAAAAAA
TCTAATTTACAAAAGAAAGACTCAAACTCAGATATTAGTATGGAAACAGA
GGTTTTCTGTGAAGGACATGAAAAAAGAGAAGAAAAGGAGTTTACGAAAC
CTATTACGGAGTATGATGCACCTAAGAAACAAGAAATAAGGGAACAGTCA
CGCAAGAAGAATGACATAGATTATAAAAAAGAAGAGGAAGAAACGGAGCT
GCAAGTTCAGCTAGGACAACGAACTAAACGAGAGATCAAAACTTCGAAGA
AAAATGAAAAGGAGAAAGAAACCAATGAATGTCACATTGAAGTAGATCAA
ATGACGAATGAAAAGCAAGGGGAGGAAAGTACAGGAAAACTGATTTCTAC
GGAAGACGTCACTTCTAAAAAAGATACAGATAAATTCTCGCATTTGTTTG
AAGGATTATCTGATAATGATGATCACATTAATGACGAAAAACCGGCAGTT
AATTCCAAATATACCACACCCAAGACGTCTCAAAATATTACGAGTGGCGT
TGATACTCCAACCACCGCGCAAACGCAAGTTTTTATGCCATCAAGTGGCA
ATTCAAGGTTAGCAAAGACACAAGCTGCGAAGAGATTGCATACAGATATT
GAATCATTAAATGAATTTCAGAAAAACTTTAAAAGGAAGAGAATTGATAG
CGAGGAGATTTCATTGTCCCAAGATGTTGAAAGGTCAAATAATAACAAAG
AACTGGCTACTAAAGCGGAAAAGATATTAGCTAGATTTAATGAACTACCA
AATTATGACCTAAAAGCGGTTTGCACCGGTTGTTTCCATGATGGATTTAA
TGAGGTTGACATCGAAATATTGAACCAACTAGGTATAAAAATTTTTGACA
ATATTAAAGAAACGGATAAATTGAATTGTATTTTTGCTCCAAAGATTTTG
AGAACCGAGAAATTTTTAAAAAGCTTAAGTTTTGAACCGTTAAAATTCGC
ATTGAAACCTGAATTTATTATTGATTTACTGAAACAAATTCATTCTAAAA
AAGATAAACTTTCTCAAATAAATATAAACTTGTTTGATTATGAGATAAAT
GGAATTAATGAGAGTATAATATCAAAAACCAAACTACCTACTAAGGTGTT
CGAAAGAGCGAATATTAGATGCATTAACTTAGTCAATGATATACCGGGTG
GTGTAGATACAATAGGCTCTGTTCTAAAGGCACATGGTATCGAAAAAATC
AACGTTTTACGTAGTAAGAAATGCACATTTGAAGACATAATCCCAAATGA
CGTTTCTAAGCAGGAAAACGGAGGCATTTTTAAATACGTACTTATCGTCA
CCAAAGCTTCACAGGTTAAAAAATTTACCAAACTGATTAATGATCGTGAT
AAAAACGAAACAATTTTGATTGTTGAATGGAATTGGTGTGTTGAATCAAT
TTTTCATCTCAATGTTGATTTTACATCAAAGAAAAATGTTTTGTATCAAA
AGAAAAATAACTAAGTCGAC

Amino Acid Sequence

MAGRRIPGLINGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLN
GEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDL
NGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEED
LNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLINHTMGTSLLFEQLNF
LILVAAEAELPIAHSTRKLLMDNSCNNCQIYELYNENLKDVKTDKDWFMN
KFGPQTVHFVISNTINFPFYKIVYFDLLIPVVSHTWVQDSVKTKRHLRTN
MYSPNPFHLLRDCQVYISKSSFNKCEYILYSDLLHLLGGTLVNYISNRTT
HVIVQSPQDPIIATVSKLTFGSFSSSSTNKHTEKPLREWKFVYPIWILYH
FKMAKPLKGELATLCELDMQDTSEEQLFAKWEEVIGDKQTSSSQLTLHPN
KTLFKNHHFAISPDLNFFTPLYWFLKGFIEDLDGKVTPLSFSDDLKSVYQ
AFPDIDCYIGHSANSPILEKTKSIKPEIHVGNVSWLFYMFALQKFTPVSQ
CKLIHQPFHAKLFTSKELTVAYTNYFGSQRFYIQRLVEILGGLSTPELTR
KNTHLITKSTIGKKFKVAKKWSLDPQNAIIVTNHMWLEQCYMNNSKLNPK
DSRFQNFKLDDNMGWNIGQIGMDHSSLPTPKNLSMVTYDTQSISEKPPPT
NDQMDQINDNTNVLSKKDGTPISSFENSIDEKIDKLQKISGEVAVTHSGD
LERSFVSRPSRASFPVVDSKKSNLQKKDSNSDISMETEVFCEGHEKREEK
EFTKPITEYDAPKKQEIREQSRKKNDIDYKKEEEETELQVQLGQRTKREI
KTSKKNEKEKETNECHIEVDQMTNEKQGEESTGKLISTEDVTSKKDTDKF
SHLFEGLSDNDDHINDEKPAVNSKYTTPKTSQNITSGVDTPTTAQTQVFM
PSSGNSRLAKTQAAKRLHTDIESLNEFQKNFKRKRIDSEEISLSQDVERS
NNNKELATKAEKILARFNELPNYDLKAVCTGCFHDGFNEVDIEILNQLGI
KIFDNIKETDKLNCIFAPKILRTEKFLKSLSFEPLKFALKPEFIIDLLKQ
IHSKKDKLSQININLFDYEINGINESIISKTKLPTKVFERANIRCINLVN
DIPGGVDTIGSVLKAHGIEKINVLRSKKCTFEDIIPNDVSKQENGGIFKY
VLIVTKASQVKKFTKLINDRDKNETILIVEWNWCVESIFHLNVDFTSKKN
VLYQKKNN*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11222
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee