cDNA Clone AS160

• Synonyms: TBC1D4
• Plasmid: pCTAP AS160(A) 1-916
• Parent Plasmid: pCTAP-A
• Expressed: AS160(A) 1-916-TAP
• Genbank: NM_014832
• Tag: TAP
• Species: Human

Sequence of Insert

ggatccgccaccatggagccgcccagctgcattcaggatgagccgttcccgcaccccctggagcccgagccgggcgtctcagctcagcccggccccgggaagccaagcgataagcggttccggctgtggtacgttggggggtcgtgcctggaccacaggaccacgctgcctatgctgccctggctcatggccgagatccgcaggcgcagccagaagcccgaggcgggcggctgcggggcgccggcggcccgagaggtgatcctggtgctcagcgcgcccttcctgcgttgcgtccccgcgccgggcgctggggcctcggggggcactagtccgtcggccacgcagcccaacccggcggtattcatcttcgagcacaaggcgcagcatatctcgcgcttcatccacaacagccacgacctcacctactttgcctacctgatcaaggcgcagcccgacgaccccgagtcgcagatggcctgccacgttttccgcgccacagaccccagccaggttcctgatgttattagcagcataaggcaattatctaaagcggccatgaaagaggatgccaaacccagcaaagataatgaggacgccttttacaactctcag
aagttcgaagtcctgtactgtggaaaggtgaccgtgacccacaagaaggc
cccctcaagcctcatcgatgactgcatggagaagttcagcctgcacgaac
agcagcgcctgaagatccaaggcgagcagcgcggtccggacccaggagag
gacctggctgacttggaggtggtggtgcccgggtcccccggagactgcct
gccggaggaggctgacggcaccgacacccaccttggcttacctgccgggg
ccagccagcctgccctgaccagctctcgggtctgcttccctgagcggatt
ttggaagattctggctttgatgagcagcaggagtttcggtctcggtgcag
cagtgtcaccggcgtgcaacggagagttcacgagggcagccagaaatccc
agccgcgacggagacacgcgagcgcacccagtcacgtccagccctcggac
tcggagaagaacaggaccatgctcttccaggttgggcgatttgagattaa
ccttatcagtccagacactaaatcagttgtgctagaaaagaattttaaag
atatctccccttgttctcagggtataaagcatgtggatcactttggcttt
atctgccgggagtctccagagcctggacttagccagtatatttgttatgt
attccagtgtgccagcgaatctctggttgatgaggtaatgctgactctga
aacaggccttcagtacggcggctgccctgcagagtgccaagacgcagatt
aaactgtgtgaggcctgcccgatgcactctttgcataagctctgtgaaag
gattgaaggtctctacccaccaagagccaagctggtgatacagaggcatc
tctcatcactgacagataatgagcaagctgacatctttgaaagagttcag
aaaatgaagccagtcagtgaccaggaagaaaatgaacttgtgattttaca
cctgaggcagctgtgtgaagccaagcagaagacacacgtgcacatcgggg
aaggccctgctactatttcaaatagtacaatcccagaaaatgcaacaagc
agtggaaggttcaaacttgacattctgaaaaataaagctaagagatcctt
aactagctccctggaaaatatcttctcaaggggagctaacagaatgagag
gtcggcttggaagtgtggacagttttgaacggtccaacagtcttgcttca
gagaaggactactcaccaggggattctccaccagggacaccgccagcgtc
cccaccgtcctcagcttggcaaacgtttcccgaagaggattccgactccc
cgcagtttcgaagacgggcacacacgttcagccacccaccttcaagcaca
aagagaaagctgaatttgcaggatgggagggctcagggtgtgcgttcccc
tctgctgaggcagagctccagtgaacagtgcagtgatggagaagggagaa
aaaggacctcatctacctgcagcaatgagtccctaagtgtgggaggaacc
tctgtcactcctcgccggatctcctggcggcagcgcattttcctcagggt
tgcttctcccatgaacaaatctccctcagcaatgcaacagcaagatggat
tggacaggaacgagctgctgccactgtcccccctctctccaaccatggag
gaggaaccgctggttatattcctgtctggggaggatgacccagaaaagat
tgaagaaagaaagaaatcaaaagaactgaggagcttgtggagaaaagcta
tacaccaacaaatcttgttacttcgaatggaaaaagaaaaccagaaactt
gaagcaagcagagatgaactccagtccagaaaagttaaattagactatga
agaagttggtgcatgtcagaaagaggtcttaataacttgggataagaagt
tgttaaactgcagagctaaaatcagatgtgatatggaagatattcatact
cttcttaaagaaggagttcccaaaagtcgacgaggagaaatttggcagtt
tctggctttacagtaccgactcagacacagattgcctaataaacaacagc
ctcctgacatatcctataaggaacttttgaggcagctcactgctcagcag
catgcgattcttgtggatttaggaaggacgtttcctactcacccttacgaattc

Amino Acid Sequence

MEPPSCIQDEPFPHPLEPEPGVSAQPGPGKPSDKRFRLWYVGGSCLDHRT
TLPMLPWLMAEIRRRSQKPEAGGCGAPAAREVILVLSAPFLRCVPAPGAG
ASGGTSPSATQPNPAVFIFEHKAQHISRFIHNSHDLTYFAYLIKAQPDDP
ESQMACHVFRATDPSQVPDVISSIRQLSKAAMKEDAKPSKDNEDAFYNSQ
KFEVLYCGKVTVTHKKAPSSLIDDCMEKFSLHEQQRLKIQGEQRGPDPGE
DLADLEVVVPGSPGDCLPEEADGTDTHLGLPAGASQPALTSSRVCFPERI
LEDSGFDEQQEFRSRCSSVTGVQRRVHEGSQKSQPRRRHASAPSHVQPSD
SEKNRTMLFQVGRFEINLISPDTKSVVLEKNFKDISPCSQGIKHVDHFGF
ICRESPEPGLSQYICYVFQCASESLVDEVMLTLKQAFSTAAALQSAKTQI
KLCEACPMHSLHKLCERIEGLYPPRAKLVIQRHLSSLTDNEQADIFERVQ
KMKPVSDQEENELVILHLRQLCEAKQKTHVHIGEGPATISNSTIPENATS
SGRFKLDILKNKAKRSLTSSLENIFSRGANRMRGRLGSVDSFERSNSLAS
EKDYSPGDSPPGTPPASPPSSAWQTFPEEDSDSPQFRRRAHTFSHPPSST
KRKLNLQDGRAQGVRSPLLRQSSSEQCSDGEGRKRTSSTCSNESLSVGGT
SVTPRRISWRQRIFLRVASPMNKSPSAMQQQDGLDRNELLPLSPLSPTME
EEPLVIFLSGEDDPEKIEERKKSKELRSLWRKAIHQQILLLRMEKENQKL
EASRDELQSRKVKLDYEEVGACQKEVLITWDKKLLNCRAKIRCDMEDIHT
LLKEGVPKSRRGEIWQFLALQYRLRHRLPNKQQPPDISYKELLRQLTAQQ
HAILVDLGRTFPTHPYEFDIKLIDTVDLEGSGSGTMDEKTTGWRGGHVVE
GLAGELEQLRARLEHHPQGQREPSGGCKLGSGKRRWKKNFIAVSAANRFK
KISSSGAL*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU11219
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression vector for expression of AS160(A) 1-916 with C-terminal TAP tag.; Sequence differences in AS160 CDS compared with NM_014832:; T1159c (S387P); T1609g (S537A); Delta nt 2034-2222 (delta aa N679-S741) (The cloned sequence represents a splice variant lacking amino acids 679-741. The majority of ESTs in the NCBI database represent this form.); Delta nt 2594-2596 (delta aa G865)