cDNA Clone ESC4 (yeast)

• Plasmid: pRS405 RTT-FL (ATG=Nco1)
• Parent Plasmid: pRS405
• Genbank: NP_012024
• Species: Yeast

Sequence of Insert

GGATCCTCTCCAATAAGATATCCCAATAAGCTATTGAGAATTTCAAGCGC
GTGAAATTCAAATTTATCATCAGAGAACGATGTGCGGGAACTCCAGGGCT
TTTCTGTATTTTCTCATAGACATTATTTATCAGTAATTGCAGCTGTGTAA
CAATAAAATTGACGCCACTTTCTTCGTCTTGTAGCTCCTCGTTAAGCTGC
GAAATTAACCTGCTCGTATTCACAGTAACACACTTAACCACAGAATGTTC
TTCGACATTAGAAATCTCTTGTATTTTTTGTACATCCCAAAAGAATTCAG
ACATTTCTATTGAACCATTCCAGAAATGTTGTTCCAGAAGTTTATTCTCT
TGAACCTTTACACTATTTCGTATTTTCTCGGTTTTAGCCGCCCACTTTTC
ACGCGTTAACAGGGTAAGCTATATCAGATGTGACTTTTCTTTTTCACTCA
TCTAATGTTGTTTTTCGCGTCACATTTTTTGCCAATAAATAAATACATCA
CAATGTTCTATATTGTATATTCAATGGAAGTCATTAGGTTATTGGGCAAG
ATTTCAAGGAGGTAGCATACTGGTAAATATACGATCCATGGGCACATCAC
TTCTGTTTGAGCAGCTAAATTTTTTAATTTTAGTGGCAGCAGAGGCTGAG
CTTCCCATCGCTCACTCTACTAGAAAGCTTTTGATGGATAATTCATGCAA
TAACTGTCAAATATACGAGCTTTATAACGAAAATTTGAAAGATGTCAAGA
CGGATAAAGATTGGTTCATGAACAAATTCGGCCCGCAAACAGTACATTTT
GTCATTAGTAATACAATCAACTTTCCTTTTTATAAGATAGTTTATTTCGA
TCTTTTGATCCCGGTGGTATCACACACTTGGGTCCAGGATTCGGTCAAGA
CGAAGAGACATTTGAGGACAAATATGTATTCACCTAACCCATTTCATTTG
CTCCGAGACTGTCAAGTATATATTTCCAAAAGCTCGTTTAATAAGTGTGA
ATACATATTATACTCTGATTTGCTTCATTTATTAGGAGGCACTTTAGTCA
ATTACATCTCAAATAGAACGACGCATGTGATTGTTCAAAGTCCCCAGGAC
CCGATCATAGCAACGGTCAGTAAATTGACATTTGGCTCTTTTTCCTCTTC
ATCGACCAACAAGCACACCGAAAAGCCTTTGAGAGAGTGGAAATTTGTCT
ATCCAATATGGATATTATATCATTTCAAAATGGCGAAGCCCTTGAAGGGC
GAGCTTGCTACTTTGTGTGAGCTTGATATGCAGGACACCAGTGAGGAACA
GTTATTCGCAAAATGGGAAGAGGTTATTGGCGATAAGCAGACATCATCAT
CGCAGCTTACTCTTCATCCAAACAAAACTTTATTCAAAAATCACCACTTT
GCTATCTCACCAGATTTAAACTTTTTCACACCTTTGTATTGGTTTTTGAA
AGGATTTATCGAGGATTTGGACGGCAAAGTAACTCCGTTGAGTTTTTCTG
ACGATCTGAAGTCTGTTTACCAAGCTTTTCCTGATATTGACTGTTATATC
GGCCACTCTGCTAACTCACCGATACTAGAAAAAACCAAAAGCATTAAACC
GGAAATTCATGTTGGGAACGTAAGTTGGTTGTTTTATATGTTTGCGCTGC
AAAAATTTACACCTGTATCGCAATGTAAACTTATTCACCAACCATTTCAT
GCGAAATTATTTACTTCTAAGGAATTAACAGTCGCGTACACTAATTATTT
CGGCTCCCAAAGGTTTTATATTCAGAGGTTAGTAGAAATATTAGGAGGAC
TTAGCACACCTGAACTAACAAGAAAAAACACACACTTGATCACGAAGAGT
ACAATTGGCAAAAAATTCAAAGTCGCAAAAAAATGGTCACTGGACCCGCA
AAATGCCATTATTGTGACAAATCATATGTGGTTAGAGCAATGTTATATGA
ATAATTCCAAGTTAAACCCCAAAGACAGTAGATTTCAAAATTTTAAATTG
GATGATAACATGGGATGGAATATCGGCCAAATAGGAATGGATCATTCATC
TCTCCCAACTCCAAAAAACCTATCGATGGTGACTTACGACACGCAATCCA
TATCAGAGAAGCCGCCTCCCACAAATGATCAAATGGATCAAATTAACGAT
AACACCAATGTATTATCAAAGAAAGATGGTACGCCCATTAGTAGTTTTGA
GAACTCAATTGATGAAAAGATTGACAAATTACAAAAAATATCAGGAGAGG
TGGCTGTAACCCATTCTGGAGATTTAGAACGGAGTTTTGTTTCCAGACCT
TCAAGGGCAAGTTTTCCAGTAGTTGACAGTAAAAAATCTAATTTACAAAA
GAAAGACTCAAACTCAGATATTAGTATGGAAACAGAGGTTTTCTGTGAAG
GACATGAAAAAAGAGAAGAAAAGGAGTTTACGAAACCTATTACGGAGTAT
GATGCACCTAAGAAACAAGAAATAAGGGAACAGTCACGCAAGAAGAATGA
CATAGATTATAAAAAAGAAGAGGAAGAAACGGAGCTGCAAGTTCAGCTAG
GACAACGAACTAAACGAGAGATCAAAACTTCGAAGAAAAATGAAAAGGAG
AAAGAAACCAATGAATGTCACATTGAAGTAGATCAAATGACGAATGAAAA
GCAAGGGGAGGAAAGTACAGGAAAACTGATTTCTACGGAAGACGTCACTT
CTAAAAAAGATACAGATAAATTCTCGCATTTGTTTGAAGGATTATCTGAT
AATGATGATCACATTAATGACGAAAAACCGGCAGTTAATTCCAAATATAC
CACACCCAAGACGTCTCAAAATATTACGAGTGGCGTTGATACTCCAACCA
CCGCGCAAACGCAAGTTTTTATGCCATCAAGTGGCAATTCAAGGTTAGCA
AAGACACAAGCTGCGAAGAGATTGCATACAGATATTGAATCATTAAATGA
ATTTCAGAAAAACTTTAAAAGGAAGAGAATTGATAGCGAGGAGATTTCAT
TGTCCCAAGATGTTGAAAGGTCAAATAATAACAAAGAACTGGCTACTAAA
GCGGAAAAGATATTAGCTAGATTTAATGAACTACCAAATTATGACCTAAA
AGCGGTTTGCACCGGTTGTTTCCATGATGGATTTAATGAGGTTGACATCG
AAATATTGAACCAACTAGGTATAAAAATTTTTGACAATATTAAAGAAACG
GATAAATTGAATTGTATTTTTGCTCCAAAGATTTTGAGAACCGAGAAATT
TTTAAAAAGCTTAAGTTTTGAACCGTTAAAATTCGCATTGAAACCTGAAT
TTATTATTGATTTACTGAAACAAATTCATTCTAAAAAAGATAAACTTTCT
CAAATAAATATAAACTTGTTTGATTATGAGATAAATGGAATTAATGAGAG
TATAATATCAAAAACCAAACTACCTACTAAGGTGTTCGAAAGAGCGAATA
TTAGATGCATTAACTTAGTCAATGATATACCGGGTGGTGTAGATACAATA
GGCTCTGTTCTAAAGGCACATGGTATCGAAAAAATCAACGTTTTACGTAG
TAAGAAATGCACATTTGAAGACATAATCCCAAATGACGTTTCTAAGCAGG
AAAACGGAGGCATTTTTAAATACGTACTTATCGTCACCAAAGCTTCACAG
GTTAAAAAATTTACCAAACTGATTAATGATCGTGATAAAAACGAAACAAT
TTTGATTGTTGAATGGAATTGGTGTGTTGAATCAATTTTTCATCTCAATG
TTGATTTTACATCAAAGAAAAATGTTTTGTATCAAAAGAAAAATAACTAA
GTCGAC

Amino Acid Sequence

MSTSLLFEQLNFLILVAAEAELPIAHSTRKLLMDNSCNNCQIYELYNENL
KDVKTDKDWFMNKFGPQTVHFVISNTINFPFYKIVYFDLLIPVVSHTWVQ
DSVKTKRHLRTNMYSPNPFHLLRDCQVYISKSSFNKCEYILYSDLLHLLG
GTLVNYISNRTTHVIVQSPQDPIIATVSKLTFGSFSSSSTNKHTEKPLRE
WKFVYPIWILYHFKMAKPLKGELATLCELDMQDTSEEQLFAKWEEVIGDK
QTSSSQLTLHPNKTLFKNHHFAISPDLNFFTPLYWFLKGFIEDLDGKVTP
LSFSDDLKSVYQAFPDIDCYIGHSANSPILEKTKSIKPEIHVGNVSWLFY
MFALQKFTPVSQCKLIHQPFHAKLFTSKELTVAYTNYFGSQRFYIQRLVE
ILGGLSTPELTRKNTHLITKSTIGKKFKVAKKWSLDPQNAIIVTNHMWLE
QCYMNNSKLNPKDSRFQNFKLDDNMGWNIGQIGMDHSSLPTPKNLSMVTY
DTQSISEKPPPTNDQMDQINDNTNVLSKKDGTPISSFENSIDEKIDKLQK
ISGEVAVTHSGDLERSFVSRPSRASFPVVDSKKSNLQKKDSNSDISMETE
VFCEGHEKREEKEFTKPITEYDAPKKQEIREQSRKKNDIDYKKEEEETEL
QVQLGQRTKREIKTSKKNEKEKETNECHIEVDQMTNEKQGEESTGKLIST
EDVTSKKDTDKFSHLFEGLSDNDDHINDEKPAVNSKYTTPKTSQNITSGV
DTPTTAQTQVFMPSSGNSRLAKTQAAKRLHTDIESLNEFQKNFKRKRIDS
EEISLSQDVERSNNNKELATKAEKILARFNELPNYDLKAVCTGCFHDGFN
EVDIEILNQLGIKIFDNIKETDKLNCIFAPKILRTEKFLKSLSFEPLKFA
LKPEFIIDLLKQIHSKKDKLSQININLFDYEINGINESIISKTKLPTKVF
ERANIRCINLVNDIPGGVDTIGSVLKAHGIEKINVLRSKKCTFEDIIPND
VSKQENGGIFKYVLIVTKASQVKKFTKLINDRDKNETILIVEWNWCVESI
FHLNVDFTSKKNVLYQKKNN*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU11216
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee