cDNA Clone TBC1D1

• Plasmid: pGBKT7 TBC1D1
• Parent Plasmid: pGBKT7
• Expressed: GAL4-DNA-BD TBC1D1
• Genbank: NM_015173
• Tag: GAL4 DNA-BD
• Species: Human

Sequence of Insert

ccatggaaccaataacattcacagcaaggaaacatctgctttctaacgaggtctcggtggattttggcctgcagctggtgggctccctgcctgtgcattccctgaccaccatgcccatgctgccctgggttgtggctgaggtgcgaagactcagcaggcagtccaccagaaaggaacctgtaaccaagcaagtccggctttgcgtttcaccctctggactgagatgtgaacctgagccagggagaagtcaacagtgggatcccctgatctattccagcatctttgagtgcaagcctcagcgtgttcacaaactgattcacaacagtcatgacccaagttactttgcttgtctgattaaggaagacgctgtccaccggcagagtatctgctatgtgttcaaagccgatgatcaaacaaaagtgcctgagatcatcagctccatccgtcaggcggggaagatcgcccggcaggaggagctgcactgcccgtccgagttcgacgacacgtttt
ccaagaagttcgaggtgctcttctgcggccgcgtgacggtggcgcacaag
aaggctccgccggccctgatcgacgagtgcatcgagaagttcaatcacgt
cagcggcagccgggggtccgagagcccccgccccaacccgccccatgccg
cgcccacagggagccaggagcctgtgcgcaggcccatgcgcaagtccttc
tcccagcccggcctgcgctcgctggcctttaggaaggagctgcaggatgg
gggcctccgaagcagcggcttcttcagctccttcgaggagagcgacattg
agaaccacctcattagcggacacaatattgtgcagcccacagatatcgag
gaaaatcgaactatgctcttcacgattggccagtctgaagtttacctcat
cagtcctgacaccaaaaaaatagcattggagaaaaattttaaggagatat
ccttttgctctcagggcatcagacacgtggaccactttgggtttatctgt
cgggagtcttccggaggtggcggctttcattttgtctgttacgtgtttca
gtgcacaaatgaggctctggttgatgaaattatgatgaccctgaaacagg
ccttcacggtggccgcagtgcagcagacagctaaggcgccagcccagctg
tgtgagggctgccccctgcaaagcctgcacaagctctgtgagaggataga
gggaatgaattcttccaaaacaaaactagaactgcaaaagcacctgacga
cattaacaaatcaggagcaggcgactatttttgaagaggttcagaaattg
agaccgagaaatgagcagcgagagaatgaattgattatttcttttctgag
atgtttatatgaagagaaacagaaagaacacatccatattggggagatga
agcagacatcgcagatggcagcagagaatattggaagtgaattaccaccc
agtgccactcgatttaggctagatatgctgaaaaacaaagcaaagagatc
tttaacagagtctttagaaagtattttgtcccggggtaataaagccagag
gcctgcaggaacactccatcagtgtggatctggatagctccctgtctagt
acattaagtaacaccagcaaagagccatctgtgtgtgaaaaggaggcctt
gcccatctctgagagctcctttaagctcctcggctcctcggaggacctgt
ccagtgactcggagagtcatctcccagaagagccagctccgctgtcgccc
cagcaggccttcaggaggcgagcaaacaccctgagtcacttccccatcga
atgccaggaacctccacaacctgcccgggggtccccgggggtttcgcaaa
ggaaacttatgaggtatcactcagtgagcacagagacgcctcatgaacga
aaggactttgaatccaaagcaaaccatcttggtgattctggtgggactcc
tgtgaagacccggaggcattcctggaggcagcagatattcctccgagtag
ccaccccgcagaaggcgtgcgattcttccagcagatatgaagattattca
gagctgggagagcttcccccacgatctcctttagaaccagtttgtgaaga
tgggccctttggccccccaccagaggaaaagaaaaggacatctcgtgagc
tccgagagctgtggcaaaaggctattcttcaacagatactgctgcttaga
atggagaaggaaaatcagaagctccaagcctctgaaaatgatttgctgaa
caagcgcctgaagctcgattatgaagaaattactccctgtcttaaagaag
taactacagtgtgggaaaagatgcttagcactccaggaagatcaaaaatt
aagtttgacatggaaaaaatgcactcggctgttgggcaaggtgtgccacg
tcatcaccgaggtgaaatctggaaatttctagctgagcaattccacctta
aacaccagtttcccagcaaacagcagccaaaggatgtgccatacaaagaa
ctcttaaagcagctgacttcccagcagcatgcgattcttattgaccttgg
gcgaacctttcctacacacccatacttctctgcccagcttggagcaggac
agctatcgctttacaacattttgaaggcctactcacttctagaccaggaa
gtgggatattgccaaggtctcagctttgtagcaggcattttgcttcttca
tatgagtgaggaagaggcgtttaaaatgctcaagtttctgatgtttgaca
tggggctgcggaaacagtatcggccagacatgattattttacagatccag
atgtaccagctctcgaggttgcttcatgattaccacagagacctctacaa
tcacctggaggagcacgagatcggccccagcctctacgctgccccctggt
tcctcaccatgtttgcctcacagttcccgctgggattcgtagccagagtc
tttgatatgatttttcttcagggaacagaggtcatatttaaagtggcttt
aagtctgttgggaagccataagcccttgattctgcagcatgaaaacctag
aaaccatagttgactttataaaaagcacgctacccaaccttggcttggta
cagatggaaaagaccatcaatcaggtatttgaaatggacatcgctaaaca
gttacaagcttatgaagttgagtaccacgtccttcaagaagaacttatcg
attcctctcctctcagtgacaaccaaagaatggataaattagagaaaacc
aacagcagcttacgcaaacagaaccttgacctccttgaacagttgcaggt
ggcaaatggtaggatccaaagccttgaggccaccattgagaagctcctga
gcagtgagagcaagctgaagcaggccatgcttaccttagaactggagcgg
tcggccctgctgcagacggtggaggagctgcggcggcggagcgcagagcc
cagcgaccgggagcctgagtgcacgcagcccgagcccacgggcgactgaccatgg

Amino Acid Sequence

MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKRSPLT
RAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQD
NVNKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPEF
VIRLTIGRAAIMEEQKLISEEDLHMAMEAEF

MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKRSPLT
RAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQD
NVNKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPEF
VIRLTIGRAAIMEEQKLISEEDLHMAMEPITFTARKHLLSNEVSVDFGLQ
LVGSLPVHSLTTMPMLPWVVAEVRRLSRQSTRKEPVTKQVRLCVSPSGLR
CEPEPGRSQQWDPLIYSSIFECKPQRVHKLIHNSHDPSYFACLIKEDAVH
RQSICYVFKADDQTKVPEIISSIRQAGKIARQEELHCPSEFDDTFSKKFE
VLFCGRVTVAHKKAPPALIDECIEKFNHVSGSRGSESPRPNPPHAAPTGS
QEPVRRPMRKSFSQPGLRSLAFRKELQDGGLRSSGFFSSFEESDIENHLI
SGHNIVQPTDIEENRTMLFTIGQSEVYLISPDTKKIALEKNFKEISFCSQ
GIRHVDHFGFICRESSGGGGFHFVCYVFQCTNEALVDEIMMTLKQAFTVA
AVQQTAKAPAQLCEGCPLQSLHKLCERIEGMNSSKTKLELQKHLTTLTNQ
EQATIFEEVQKLRPRNEQRENELIISFLRCLYEEKQKEHIHIGEMKQTSQ
MAAENIGSELPPSATRFRLDMLKNKAKRSLTESLESILSRGNKARGLQEH
SISVDLDSSLSSTLSNTSKEPSVCEKEALPISESSFKLLGSSEDLSSDSE
SHLPEEPAPLSPQQAFRRRANTLSHFPIECQEPPQPARGSPGVSQRKLMR
YHSVSTETPHERKDFESKANHLGDSGGTPVKTRRHSWRQQIFLRVATPQK
ACDSSSRYEDYSELGELPPRSPLEPVCEDGPFGPPPEEKKRTSRELRELW
QKAILQQILLLRMEKENQKLQASENDLLNKRLKLDYEEITPCLKEVTTVW
EKMLSTPGRSKIKFDMEKMHSAVGQGVPRHHRGEIWKFLAEQFHLKHQFP
SKQQPKDVPYKELLKQLTSQQHAILIDLGRTFPTHPYFSAQLGAGQLSLY
NILKAYSLLDQEVGYCQGLSFVAGILLLHMSEEEAFKMLKFLMFDMGLRK
QYRPDMIILQIQMYQLSRLLHDYHRDLYNHLEEHEIGPSLYAAPWFLTMF
ASQFPLGFVARVFDMIFLQGTEVIFKVALSLLGSHKPLILQHENLETIVD
FIKSTLPNLGLVQMEKTINQVFEMDIAKQLQAYEVEYHVLQEELIDSSPL
SDNQRMDKLEKTNSSLRKQNLDLLEQLQVANGRIQSLEATIEKLLSSESK
LKQAMLTLELERSALLQTVEELRRRSAEPSDREPECTQPEPTGD*

• Vector Type: Other
• Antibiotic: Kan
• DU Number: DU11213
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: MATCHMAKER yeast Two-Hybrid vector.; Sequence difference compared with NM_015173 CDS:; C1266a (silent in T422)