cDNA Clone EMAPL

• Plasmid: pCMV5-HA3 EMAPL (P)
• Parent Plasmid: pCMV5 HA3
• Expressed: HA-EMAPL (P)
• Genbank: *See notes
• Tag: HA
• Species: Human

Sequence of Insert

gaattcgcccttcgaatccatggacccccagcccctcagggggccagcgaagagcctagtgggacccaatctgaaggagggggcagcagcagcagtggtgctggctcccctggccccccggggatcctcaggcccttgcagcccccacagcgtgctgacaccgcgaagaaattcttcctcctcctcatccccctcagagcggcctcggcagaagctctccaggaaggcaatctcctccgccaacctgttagtgcggtccgggagcacagagagccgtgggggaaaagaccccctctccagccctgggggccctggatctcggaggagcaattacaatttggaaggcatctcagtgaagatgttccttcgagggcgccccattaccatgtacatcccgtctggcatccgcagccttgaggagctgccgagtggcccaccgccagagaccctcagccttgactgggtttatgggtacaggggtcgtgactcccgctctaatctgtttgtgttgcgctctggggaggtggtctactttatcgcctgtgtggtggtgctgtaccggcctggaggaggcccagggggtcctggaggtggcggccagagacattaccgggggcacacagactgcgttcgatgccttgctgttcaccctgatggtgttcgggtagcctcgggacagacagctggagtggataaggatggaaagcccctgcagcctgtggttcacatctgggactcagagacgctgttgaaactgcaggagattggactgggggccttcgagcggggtgttggggccctggccttttcagctgcggatcagggtgcctttctttgtgtggtggatgattccaatgagcacatgctgtcggtgtgggactgcagccggggaatgaagctggctgagatcaagagtacaaatgactcagtcttggccgttggcttcaaccctcgtgacagcagctgcat
cgtcaccagtgggaaatctcacgtccacttctggaattggagtggtggag
taggggttcctgggaatgggacccttacccggaaacagggtgtctttggg
aaatacaagaaacccaagtttatcccttgctttgtgttccttccggatgg
agacattctcaccggagactcagaggggaacattctcacctgggggcgga
gcccttcagattccaagaccccaggcaggggtggcgccaaagagacctat
gggattgtggcccaggctcacgctcatgaaggttctatcttcgccttgtg
tctccggagggacgggacagtgctgagtggtggcgggcgggaccgccggc
tggtacagtgggggcccgggttggtggccctccaggaggctgagattccc
gagcacttcggggccgtgcgagccattgctgaagggcttggctctgagct
gctggtgggaaccacgaagaatgcattgctgaggggagacctggcccagg
gcttctcccctgtaatccagggccacactgatgagctctgggggctctgc
acacacccctcccagaaccgcttcctcacctgcggccacgaccggcagct
ctgcctgtgggatggggagagccatgcactggcctggagcatcgacctca
aggagactggtctctgtgctgacttccacccgagtggggcagttgtggcc
gtaggactgaacacggggaggtggttggttttggacacagagaccagaga
gatcgtgtctgatgtcattgatggcaatgagcagctctcagtggtccggt
acagcccagatgggttgcacctggccattggttcccatgacaacgtgatc
tacatctatagtgtctccagtgatggtgccaaatccagccgctttggccg
ctgtatgggtcactccagcttcatcactcatcttgactggtccaaggatg
ggaatttcatcatgtccaattctggggactatgagattctttactgggac
gtggctggaggctgcaagcagctgaagaatcgctatgagagccgagaccg
ggaatgggctacctacacctgtgtgctgggctttcacgtctacgtacccg
tgcgctcgtgccaaggcgccgagccgcatgtacgggggccacggcagcca
cgtgaccagcgtccgattcacgcacgacgactcgcacctcgtctcgctgg
gcggcaaggacgccagcatcttccagtggcgagtgctgggcgctgggggc
gcggggccggcgcccgccacgccctctcgaaccccctccctgtcccccgc
ctcctccctcgacgtttgatcgctgcctggcgggaccgactggcccggcg
gcgtggccccgccccgccctgcccttccctggcccaatcccccacgacta
ggggccgactctttcctggactgacttcgagacattcccgatcgcgcatt
ttcctggagggcgcgaacggcgcccctgcacacactgtttaggcggccgc

Amino Acid Sequence

MYPYDVPDYAGDPRNSPFESMDPQPLRGPAKSLVGPNLKEGAAAAVVLAP
LAPRGSSGPCSPHSVLTPRRNSSSSSSPSERPRQKLSRKAISSANLLVRS
GSTESRGGKDPLSSPGGPGSRRSNYNLEGISVKMFLRGRPITMYIPSGIR
SLEELPSGPPPETLSLDWVYGYRGRDSRSNLFVLRSGEVVYFIACVVVLY
RPGGGPGGPGGGGQRHYRGHTDCVRCLAVHPDGVRVASGQTAGVDKDGKP
LQPVVHIWDSETLLKLQEIGLGAFERGVGALAFSAADQGAFLCVVDDSNE
HMLSVWDCSRGMKLAEIKSTNDSVLAVGFNPRDSSCIVTSGKSHVHFWNW
SGGVGVPGNGTLTRKQGVFGKYKKPKFIPCFVFLPDGDILTGDSEGNILT
WGRSPSDSKTPGRGGAKETYGIVAQAHAHEGSIFALCLRRDGTVLSGGGR
DRRLVQWGPGLVALQEAEIPEHFGAVRAIAEGLGSELLVGTTKNALLRGD
LAQGFSPVIQGHTDELWGLCTHPSQNRFLTCGHDRQLCLWDGESHALAWS
IDLKETGLCADFHPSGAVVAVGLNTGRWLVLDTETREIVSDVIDGNEQLS
VVRYSPDGLHLAIGSHDNVIYIYSVSSDGAKSSRFGRCMGHSSFITHLDW
SKDGNFIMSNSGDYEILYWDVAGGCKQLKNRYESRDREWATYTCVLGFHV
YVPVRSCQGAEPHVRGPRQPRDQRPIHARRLAPRLAGRQGRQHLPVASAG
RWGRGAGARHALSNPLPVPRLLPRRLIAAWRDRLARRRGPAPPCPSLAQS
PTTRGRLFPGLTSRHSRSRIFLEGANGAPAHTV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5979
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *None (‘artificial’ composite to give 5’ end as Pawson et al, reading into ELM3(EMAPL/ELP95) – 3’ end matches AK093146) ; ; N.B. Has N277D and Y590H inherited from RT cloned EMAPL (N353D, Y666H compared with AK093146 CDS); ; Estimated MW of expressed protein : 89895 Daltons