cDNA Clone EMAPL

• Plasmid: pCMV5-HA EMAPL (1) (1st ATG version. Matches BC108280)
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-EMAPL (1)
• Genbank: BC108280
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCATGGACGGGGCCGCGGGGCCCGGTGACGGCCCTGCTCGGGAGGCCCTCCAGTCTCTGAGCCAGCGGCTTCGGGTGCAGGAGCAGGAGATGGAACTGGTAAAGGCAGCCCTGGCAGAAGCCCTTCGCCTGCTGCGGCTGCAGGTGCCCCCTTCCTCCCTGCAGGGCTCTGGCACACCAGCTCCTCCGGGGGACAGTCTTGCAGCCCCCCCAGGACTGCCACCCACGTGCACCCCTTCCTTGGTGAGCCGAGGCACCCAGACGGAGACAGAGGTGGAGCTCAAGTCATCCCCTGGACCCCCT
GGCCTGAGCAATGGACCCCCAGCCCCTCAGGGGGCCAGCGAAGAGCCTAG
CGGGACCCAATCTGAAGGAGGGGGCAGCAGCAGCAGTGGTGCTGGCTCCC
CTGGCCCCCCGGGGATCCTCAGGCCCTTGCAGCCCCCACAGCGTGCTGAC
ACGCCGCGAAGAAATTCTTCCTCCTCCTCATCCCCCTCAGAGCGGCCTCG
GCAGAAGCTCTCCAGGAAGGCAATCTCCTCCGCCAACCTGTTAGTGCGGT
CCGGGAGCACAGAGAGCCGTGGGGGAAAAGACCCCCTCTCCAGCCCTGGG
GGCCCTGGATCTCGGAGGAGCAATTACAATTTGGAAGGCATCTCAGTGAA
GATGTTCCTTCGAGGGCGCCCCATTACCATGTACATCCCGTCTGGCATCC
GCAGCCTTGAGGAGCTGCCGAGTGGCCCACCGCCAGAGACCCTCAGCCTT
GACTGGGTTTATGGGTACAGGGGTCGTGACTCCCGCTCTAATCTGTTTGT
GTTGCGCTCTGGGGAGGTGGTCTACTTTATCGCCTGTGTGGTGGTGCTGT
ACCGGCCTGGAGGAGGCCCAGGGGGTCCTGGAGGTGGCGGCCAGAGACAT
TACCGGGGGCACACAGACTGCGTTCGATGCCTTGCTGTTCACCCTGATGG
TGTTCGGGTAGCCTCGGGACAGACAGCTGGAGTGGATAAGGATGGAAAGC
CCCTGCAGCCTGTGGTTCACATCTGGGACTCAGAGACGCTGTTGAAACTG
CAGGAGATTGGACTGGGGGCCTTCGAGCGGGGTGTTGGGGCCCTGGCCTT
TTCAGCTGCGGATCAGGGTGCCTTTCTTTGTGTGGTGGATGATTCCAATG
AGCACATGCTGTCGGTGTGGGACTGCAGCCGGGGAATGAAGCTGGCTGAG
ATCAAGAGTACAAATGACTCAGTCCTGGCCGTTGGCTTCAACCCTCGTGA
CAGCAGCTGCATCGTCACCAGTGGGAAATCTCACGTCCACTTCTGGAATT
GGAGTGGTGGAGTAGGGGTTCCTGGGAATGGGACCCTTACCCGGAAACAG
GGTGTCTTTGGGAAATACAAGAAACCCAAGTTTATCCCTTGCTTTGTGTT
CCTTCCGGATGGAGACATTCTCACTGGAGACTCAGAGGGGAACATTCTCA
CCTGGGGGCGGAGCCCTTCAGATTCCAAGACCCCAGGCAGGGGTGGCGCC
AAAGAGACCTATGGGATTGTGGCCCAGGCTCACGCTCATGAAGGTTCTAT
CTTCGCCTTGTGTCTCCGGAGGGACGGGACAGTGCTGAGTGGTGGCGGGC
GGGACCGCCGGCTGGTACAGTGGGGGCCCGGGTTGGTGGCCCTCCAGGAG
GCTGAGATTCCCGAGCACTTCGGGGCCGTGCGAGCCATTGCTGAAGGGCT
TGGCTCTGAGCTGCTGGTGGGAACCACGAAGAATGCATTGCTGAGGGGAG
ACCTGGCCCAGGGCTTCTCCCCTGTAATCCAGGGCCACACTGATGAGCTC
TGGGGGCTCTGCACACACCCCTCCCAGAACCGCTTCCTCACCTGCGGCCA
CGACCGGCAGCTCTGCCTGTGGGATGGGGAGAGCCATGCACTGGCCTGGA
GCATCGACCTCAAGGAGACTGGTCTCTGTGCTGACTTCCACCCGAGTGGG
GCAGTTGTGGCCGTAGGACTGAACACGGGGAGGTGGTTGGTTTTGGACAC
AGAGACCAGAGAGATCGTGTCTGATGTCATTGATGGCAATGAGCAGCTCT
CAGTGGTCCGGTACAGCCCAGATGGGTTGTACCTGGCCATTGGTTCCCAT
GACAACGTGATCTACATCTATAGTGTTTCCAGTGATGGTGCCAAATCCAG
CCGCTTTGGCCGCTGTATGGGTCACTCCAGCTTCATCACTCATCTTGACT
GGTCCAAGGATGGGAATTTCATCATGTCCAATTCTGGGGACTATGAGATT
CTTTACTGGGACGTGGCTGGAGGCTGCAAGCAGCTGAAGAATCGCTATGA
GAGCCGAGACCGGGAATGGGCTACCTACACCTGTGTGCTGGGCTTTCACG
TCTACGGCGTCTGGCCGGACGGCTCCGATGGGACCGACATCAACTCCCTG
TGCCGCTCCCACAACGAGCGCGTGGTGGCGGTGGCCGACGACTTCTGCAA
AGTGCATCTCTTCCAGTACCCGTGCGCTCGTGCCAAGGCGCCGAGCCGCA
TGTACGGGGGCCACGGCAGCCACGTGACCAGCGTCCGATTCACGCACGAC
GACTCGCACCTCGTCTCGCTGGGCGGCAAGGACGCCAGCATCTTCCAGTG
GCGAGTGCTGGGCGCTGGGGGCGCGGGGCCGGCGCCCGCCACGCCCTCTC
GAACCCCCTCCCTGTCCCCCGCCTCCTCCCTCGACGTTTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSPEFMDGAAGPGDGPAREALQSLSQRLRVQEQEMELVKA
ALAEALRLLRLQVPPSSLQGSGTPAPPGDSLAAPPGLPPTCTPSLVSRGT
QTETEVELKSSPGPPGLSNGPPAPQGASEEPSGTQSEGGGSSSSGAGSPG
PPGILRPLQPPQRADTPRRNSSSSSSPSERPRQKLSRKAISSANLLVRSG
STESRGGKDPLSSPGGPGSRRSNYNLEGISVKMFLRGRPITMYIPSGIRS
LEELPSGPPPETLSLDWVYGYRGRDSRSNLFVLRSGEVVYFIACVVVLYR
PGGGPGGPGGGGQRHYRGHTDCVRCLAVHPDGVRVASGQTAGVDKDGKPL
QPVVHIWDSETLLKLQEIGLGAFERGVGALAFSAADQGAFLCVVDDSNEH
MLSVWDCSRGMKLAEIKSTNDSVLAVGFNPRDSSCIVTSGKSHVHFWNWS
GGVGVPGNGTLTRKQGVFGKYKKPKFIPCFVFLPDGDILTGDSEGNILTW
GRSPSDSKTPGRGGAKETYGIVAQAHAHEGSIFALCLRRDGTVLSGGGRD
RRLVQWGPGLVALQEAEIPEHFGAVRAIAEGLGSELLVGTTKNALLRGDL
AQGFSPVIQGHTDELWGLCTHPSQNRFLTCGHDRQLCLWDGESHALAWSI
DLKETGLCADFHPSGAVVAVGLNTGRWLVLDTETREIVSDVIDGNEQLSV
VRYSPDGLYLAIGSHDNVIYIYSVSSDGAKSSRFGRCMGHSSFITHLDWS
KDGNFIMSNSGDYEILYWDVAGGCKQLKNRYESRDREWATYTCVLGFHVY
GVWPDGSDGTDINSLCRSHNERVVAVADDFCKVHLFQYPCARAKAPSRMY
GGHGSHVTSVRFTHDDSHLVSLGGKDASIFQWRVLGAGGAGPAPATPSRT
PSLSPASSLDV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5954
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. sequence differences compared with BC108280 CDS: g210a, t351c, t1098c (all silent and present in other versions of EMAPL); ; Estimated MW of expressed protein : 96926 Daltons