cDNA Clone EMAPL

• Plasmid: pEBG6P-1 EMAPL (1) (1st ATG version. Matches BC108280)
• Parent Plasmid: pEBG6P
• Expressed: GST-EMAPL (1) (1st ATG version. Matches BC108280)
• Genbank: BC108280
• Tag: GST

Sequence of Insert

ACTAGTATGGACGGGGCCGCGGGGCCCGGTGACGGCCCTGCTCGGGAGGCCCTCCAGTCTCTGAGCCAGCGGCTTCGGGTGCAGGAGCAGGAGATGGAACTGGTAAAGGCAGCCCTGGCAGAAGCCCTTCGCCTGCTGCGGCTGCAGGTGCCCCCTTCCTCCCTGCAGGGCTCTGGCACACCAGCTCCTCCGGGGGACAGTCTTGCAGCCCCCCCAGGACTGCCACCCACGTGCACCCCTTCCTTGGTGAGCCGAGGCACCCAGACGGAGACAGAGGTGGAGCTCAAGTCATCCCCTGGACCCCCT
GGCCTGAGCAATGGACCCCCAGCCCCTCAGGGGGCCAGCGAAGAGCCTAG
CGGGACCCAATCTGAAGGAGGGGGCAGCAGCAGCAGTGGTGCTGGCTCCC
CTGGCCCCCCGGGGATCCTCAGGCCCTTGCAGCCCCCACAGCGTGCTGAC
ACGCCGCGAAGAAATTCTTCCTCCTCCTCATCCCCCTCAGAGCGGCCTCG
GCAGAAGCTCTCCAGGAAGGCAATCTCCTCCGCCAACCTGTTAGTGCGGT
CCGGGAGCACAGAGAGCCGTGGGGGAAAAGACCCCCTCTCCAGCCCTGGG
GGCCCTGGATCTCGGAGGAGCAATTACAATTTGGAAGGCATCTCAGTGAA
GATGTTCCTTCGAGGGCGCCCCATTACCATGTACATCCCGTCTGGCATCC
GCAGCCTTGAGGAGCTGCCGAGTGGCCCACCGCCAGAGACCCTCAGCCTT
GACTGGGTTTATGGGTACAGGGGTCGTGACTCCCGCTCTAATCTGTTTGT
GTTGCGCTCTGGGGAGGTGGTCTACTTTATCGCCTGTGTGGTGGTGCTGT
ACCGGCCTGGAGGAGGCCCAGGGGGTCCTGGAGGTGGCGGCCAGAGACAT
TACCGGGGGCACACAGACTGCGTTCGATGCCTTGCTGTTCACCCTGATGG
TGTTCGGGTAGCCTCGGGACAGACAGCTGGAGTGGATAAGGATGGAAAGC
CCCTGCAGCCTGTGGTTCACATCTGGGACTCAGAGACGCTGTTGAAACTG
CAGGAGATTGGACTGGGGGCCTTCGAGCGGGGTGTTGGGGCCCTGGCCTT
TTCAGCTGCGGATCAGGGTGCCTTTCTTTGTGTGGTGGATGATTCCAATG
AGCACATGCTGTCGGTGTGGGACTGCAGCCGGGGAATGAAGCTGGCTGAG
ATCAAGAGTACAAATGACTCAGTCCTGGCCGTTGGCTTCAACCCTCGTGA
CAGCAGCTGCATCGTCACCAGTGGGAAATCTCACGTCCACTTCTGGAATT
GGAGTGGTGGAGTAGGGGTTCCTGGGAATGGGACCCTTACCCGGAAACAG
GGTGTCTTTGGGAAATACAAGAAACCCAAGTTTATCCCTTGCTTTGTGTT
CCTTCCGGATGGAGACATTCTCACTGGAGACTCAGAGGGGAACATTCTCA
CCTGGGGGCGGAGCCCTTCAGATTCCAAGACCCCAGGCAGGGGTGGCGCC
AAAGAGACCTATGGGATTGTGGCCCAGGCTCACGCTCATGAAGGTTCTAT
CTTCGCCTTGTGTCTCCGGAGGGACGGGACAGTGCTGAGTGGTGGCGGGC
GGGACCGCCGGCTGGTACAGTGGGGGCCCGGGTTGGTGGCCCTCCAGGAG
GCTGAGATTCCCGAGCACTTCGGGGCCGTGCGAGCCATTGCTGAAGGGCT
TGGCTCTGAGCTGCTGGTGGGAACCACGAAGAATGCATTGCTGAGGGGAG
ACCTGGCCCAGGGCTTCTCCCCTGTAATCCAGGGCCACACTGATGAGCTC
TGGGGGCTCTGCACACACCCCTCCCAGAACCGCTTCCTCACCTGCGGCCA
CGACCGGCAGCTCTGCCTGTGGGATGGGGAGAGCCATGCACTGGCCTGGA
GCATCGACCTCAAGGAGACTGGTCTCTGTGCTGACTTCCACCCGAGTGGG
GCAGTTGTGGCCGTAGGACTGAACACGGGGAGGTGGTTGGTTTTGGACAC
AGAGACCAGAGAGATCGTGTCTGATGTCATTGATGGCAATGAGCAGCTCT
CAGTGGTCCGGTACAGCCCAGATGGGTTGTACCTGGCCATTGGTTCCCAT
GACAACGTGATCTACATCTATAGTGTTTCCAGTGATGGTGCCAAATCCAG
CCGCTTTGGCCGCTGTATGGGTCACTCCAGCTTCATCACTCATCTTGACT
GGTCCAAGGATGGGAATTTCATCATGTCCAATTCTGGGGACTATGAGATT
CTTTACTGGGACGTGGCTGGAGGCTGCAAGCAGCTGAAGAATCGCTATGA
GAGCCGAGACCGGGAATGGGCTACCTACACCTGTGTGCTGGGCTTTCACG
TCTACGGCGTCTGGCCGGACGGCTCCGATGGGACCGACATCAACTCCCTG
TGCCGCTCCCACAACGAGCGCGTGGTGGCGGTGGCCGACGACTTCTGCAA
AGTGCATCTCTTCCAGTACCCGTGCGCTCGTGCCAAGGCGCCGAGCCGCA
TGTACGGGGGCCACGGCAGCCACGTGACCAGCGTCCGATTCACGCACGAC
GACTCGCACCTCGTCTCGCTGGGCGGCAAGGACGCCAGCATCTTCCAGTG
GCGAGTGCTGGGCGCTGGGGGCGCGGGGCCGGCGCCCGCCACGCCCTCTC
GAACCCCCTCCCTGTCCCCCGCCTCCTCCCTCGACGTTTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSMDGAAGPGDGP
AREALQSLSQRLRVQEQEMELVKAALAEALRLLRLQVPPSSLQGSGTPAP
PGDSLAAPPGLPPTCTPSLVSRGTQTETEVELKSSPGPPGLSNGPPAPQG
ASEEPSGTQSEGGGSSSSGAGSPGPPGILRPLQPPQRADTPRRNSSSSSS
PSERPRQKLSRKAISSANLLVRSGSTESRGGKDPLSSPGGPGSRRSNYNL
EGISVKMFLRGRPITMYIPSGIRSLEELPSGPPPETLSLDWVYGYRGRDS
RSNLFVLRSGEVVYFIACVVVLYRPGGGPGGPGGGGQRHYRGHTDCVRCL
AVHPDGVRVASGQTAGVDKDGKPLQPVVHIWDSETLLKLQEIGLGAFERG
VGALAFSAADQGAFLCVVDDSNEHMLSVWDCSRGMKLAEIKSTNDSVLAV
GFNPRDSSCIVTSGKSHVHFWNWSGGVGVPGNGTLTRKQGVFGKYKKPKF
IPCFVFLPDGDILTGDSEGNILTWGRSPSDSKTPGRGGAKETYGIVAQAH
AHEGSIFALCLRRDGTVLSGGGRDRRLVQWGPGLVALQEAEIPEHFGAVR
AIAEGLGSELLVGTTKNALLRGDLAQGFSPVIQGHTDELWGLCTHPSQNR
FLTCGHDRQLCLWDGESHALAWSIDLKETGLCADFHPSGAVVAVGLNTGR
WLVLDTETREIVSDVIDGNEQLSVVRYSPDGLYLAIGSHDNVIYIYSVSS
DGAKSSRFGRCMGHSSFITHLDWSKDGNFIMSNSGDYEILYWDVAGGCKQ
LKNRYESRDREWATYTCVLGFHVYGVWPDGSDGTDINSLCRSHNERVVAV
ADDFCKVHLFQYPCARAKAPSRMYGGHGSHVTSVRFTHDDSHLVSLGGKD
ASIFQWRVLGAGGAGPAPATPSRTPSLSPASSLDV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5953
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express EMAPL(1) with N-terminal GST (contains preScission protease site); N.B. sequence differences compared with BC108280 CDS: g210a, t351c, t1098c (all silent and present in other versions of EMAPL); ; Estimated MW of expressed protein : 122798 Daltons