cDNA Clone slit-robo-GAP 1

• Plasmid: pCMV5-HA slit-robo-GAP 1 isoform 2
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-srGAP1 isoform 2
• Genbank: AB037725*
• Tag: HA
• Species: Human

Sequence of Insert

ggtaccaacagttgcagtcccgccttgccacgctcaaaatcgagaatgaa
gaggttaagaaaacgactgaagccaccttgcagacgatacaagatatggt
caccatcgaggactatgatgtttctgaatgcttccagcacagtcgttcca
cagaatctgtgaagtccactgtctctgaaacctacctgagtaaacccagc
atcgccaagagaagagccaaccagcaggaaactgaacagttctacttcat
gaaactcagagaatatttggaaggcagtaatctcatcacaaaacttcaag
ccaaacatgacttgctgcagaggaccctgggagaaggtcatagagctgaa
tatatgactacaagccgaggacgaagaaactcgcacacaagacatcagga
ctcaggacaggttattcccctcattgtggaaagctgtattcggttcatca
atctctatggtcttcagcatcaggggattttcagagtgtctggttcccag
gtggaagtcaatgatattaaaaattcatttgagagaggtgaaaatccttt
ggctgatgaccagagtaaccatgatattaactcagttgctggcgttctga
agctctatttccgtgggctggaaaaccccctctttcctaaggaaagattt
aacgatctgatttcttgtatcagaatagataatctctatgagagggcgct
tcacatccgcaaactcctcctgactttgcccaggtcggtccttatagtga
tgaggtacc

Amino Acid Sequence

MYPYDVPDYAGSMSTPSRFKKDKEIIAEYESQVKEIRAQLVEQQKCLEQQ
TEMRVQLLQDLQDFFRKKAEIETEYSRNLEKLAERFMAKTRSTKDHQQYK
KDQNLLSPVNCWYLLLNQVRRESKDHATLSDIYLNNVIMRFMQISEDSTR
MFKKSKEIAFQLHEDLMKVLNELYTVMKTYHMYHAESISAESKLKEAEKQ
EEKQIGRSGDPVFHIRLEERHQRRSSVKKIEKMKEKRQAKYSENKLKSIK
ARNEYLLTLEATNASVFKYYIHDLSDLIDCCDLGYHASLNRALRTYLSAE
YNLETSRHEGLDIIENAVDNLEPRSDKQRFMEMYPAAFCPPMKFEFQSHM
GDEVCQVSAQQPVQAELMLRYQQLQSRLATLKIENEEVKKTTEATLQTIQ
DMVTIEDYDVSECFQHSRSTESVKSTVSETYLSKPSIAKRRANQQETEQF
YFMKLREYLEGSNLITKLQAKHDLLQRTLGEGHRAEYMTTSRGRRNSHTR
HQDSGQVIPLIVESCIRFINLYGLQHQGIFRVSGSQVEVNDIKNSFERGE
NPLADDQSNHDINSVAGVLKLYFRGLENPLFPKERFNDLISCIRIDNLYE
RALHIRKLLLTLPRSVLIVMRYLFAFLNHLSQYSDENMMDPYNLAICFGP
TLMPVPEIQDQVSCQAHVNEIIKTIIIHHETIFPDAKELDGPVYEKCMAG
DDYCDSPYSEHGTLEEVDQDAGTEPHTSEDECEPIEAIAKFDYVGRSARE
LSFKKGASLLLYHRASEDWWEGRHNGIDGLVPHQYIVVQDMDDTFSDTLS
QKADSEASSGPVTEDKSSSKDMNSPTDRHPDGYLARQRKRGEPPPPVRRP
GRTSDGHCPLHPPHALSNSSVDLGSPSLASHPRGLLQNRGLNNDSPERRR
RPGHGSLTNISRHDSLKKIDSPPIRRSTSSGQYTGFNDHKPLDPETIAQD
IEETMNTALNELRELERQSTAKHAPDVVLDTLEQVKNSPTPATSTESLSP
LHNVALRSSEPQIRRSTSSSSDTMSTFKPMVAPRMGVQLKPPALRPKPAV
LPKTNPTIGPAPPPQGPTDKSCTM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5909
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Start codon not identified in AB037725. This clone starts as isoform 1 (NM_020762) starts, i.e. MSTPSRFKKDKEIIAEYESQVKEIR…; ; Estimated MW of expressed protein : 122950 Daltons