cDNA Clone ILK

• Plasmid: pFastBacHTa ILK
• Parent Plasmid: pSC-A
• Expressed: 6His-ILK
• Genbank: NM_004517
• Tag: 6His
• Species: Human

Sequence of Insert

gaattcATGGACGACATTTTCACTCAGTGCCGGGAGGGCAACGCAGTCGCCGTTCGCCTGTGGCTGGACAACACGGAGAACGACCTCAACCAGGGGGACGATCATGGCTTCTCCCCCTTGCACTGGGCCTGCCGAGAGGGCCGCTCTGCTGTGGTTGAGATGTTGATCATGCGGGGGGCACGGATCAATGTAATGAACCGTGGGGATGACACCCCCCTGCATCTGGCAGCCAGTCATGGACACCGTGATATTGTACAGAAGCTATTGCAGTACAAGGCAGACATCAATGCAGTGAATGAACATGGG
AATGTGCCCCTGCACTATGCCTGTTTTTGGGGCCAAGATCAAGTGGCAGA
GGACCTGGTGGCAAATGGGGCCCTTGTCAGCATCTGTAACAAGTATGGAG
AGATGCCTGTGGACAAAGCCAAGGCACCCCTGAGAGAGCTTCTCCGAGAG
CGGGCAGAGAAGATGGGCCAGAATCTCAACCGTATTCCATACAAGGACAC
ATTCTGGAAGGGGACCACCCGCACTCGGCCCCGAAATGGAACCCTGAACA
AACACTCTGGCATTGACTTCAAACAGCTTAACTTCCTGACGAAGCTCAAC
GAGAATCACTCTGGAGAGCTATGGAAGGGCCGCTGGCAGGGCAATGACAT
TGTCGTGAAGGTGCTGAAGGTTCGAGACTGGAGTACAAGGAAGAGCAGGG
ACTTCAATGAAGAGTGTCCCCGGCTCAGGATTTTCTCGCATCCAAATGTG
CTCCCAGTGCTAGGTGCCTGCCAGTCTCCACCTGCTCCTCATCCTACTCT
CATCACACACTGGATGCCATATGGATCCCTCTACAATGTACTACATGAAG
GCACCAATTTCGTCGTGGACCAGAGCCAGGCTGTGAAGTTTGCTTTGGAC
ATGGCAAGGGGCATGGCCTTCCTACACACACTAGAGCCCCTCATCCCACG
ACATGCACTCAATAGCCGTAGTGTAATGATTGATGAGGACATGACTGCCC
GAATTAGCATGGCTGATGTCAAGTTCTCTTTCCAATGTCCTGGTCGCATG
TATGCACCTGCCTGGGTAGCCCCCGAAGCTCTGCAGAAGAAGCCTGAAGA
CACAAACAGACGCTCAGCAGACATGTGGAGTTTTGCAGTGCTTCTGTGGG
AACTGGTGACACGGGAGGTACCCTTTGCTGACCTCTCCAATATGGAGATT
GGAATGAAGGTGGCATTGGAAGGCCTTCGGCCTACCATCCCACCAGGTAT
TTCCCCTCATGTGTGTAAGCTCATGAAAATCTGCATGAATGAAGACCCTG
CAAAGCGACCCAAATTTGACATGATTGTGCCTATCCTTGAGAAGATGCAG
GACAAGTAGgcggccgc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFMDDIFTQCREGNAVAVRLWL
DNTENDLNQGDDHGFSPLHWACREGRSAVVEMLIMRGARINVMNRGDDTP
LHLAASHGHRDIVQKLLQYKADINAVNEHGNVPLHYACFWGQDQVAEDLV
ANGALVSICNKYGEMPVDKAKAPLRELLRERAEKMGQNLNRIPYKDTFWK
GTTRTRPRNGTLNKHSGIDFKQLNFLTKLNENHSGELWKGRWQGNDIVVK
VLKVRDWSTRKSRDFNEECPRLRIFSHPNVLPVLGACQSPPAPHPTLITH
WMPYGSLYNVLHEGTNFVVDQSQAVKFALDMARGMAFLHTLEPLIPRHAL
NSRSVMIDEDMTARISMADVKFSFQCPGRMYAPAWVAPEALQKKPEDTNR
RSADMWSFAVLLWELVTREVPFADLSNMEIGMKVALEGLRPTIPPGISPH
VCKLMKICMNEDPAKRPKFDMIVPILEKMQDK*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5903
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; N.B. Sequence differences compared with NM_004517 CDS:; c297t (silent H99), g819a (silent P273), g1278a (silent K426); ; Estimated MW of expressed protein : 55133 Daltons