cDNA Clone ITK

• Plasmid: pFastBacHTb ITK
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-ITK
• Genbank: NM_005546
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGAACAACTTTATCCTCCTGGAAGAACAGCTCATCAAGAAATCCCAACAAAAGAGAAGAACTTCTCCCTCGAACTTTAAAGTCCGCTTCTTTGTGTTAACCAAAGCCAGCCTGGCATACTTTGAAGATCGTCATGGGAAGAAGCGCACGCTGAAGGGGTCCATTGAGCTCTCCCGAATCAAATGTGTTGAGATTGTGAAAAGTGACATCAGCATCCCATGCCACTATAAATACCCGTTTCAGGTGGTGCATGACAACTACCTCCTATATGTGTTTGCTCCAGATCGTGAGAGCCGGCAG
CGCTGGGTGCTGGCCCTTAAAGAAGAAACGAGGAATAATAACAGTTTGGT
GCCTAAATATCATCCTAATTTCTGGATGGATGGGAAGTGGAGGTGCTGTT
CTCAGCTGGAGAAGCTTGCAACAGGCTGTGCCCAATATGATCCAACCAAG
AATGCTTCAAAGAAGCCTCTTCCTCCTACTCCTGAAGACAACAGGCGACC
ACTTTGGGAACCTGAAGAAACTGTGGTCATTGCCTTATATGACTACCAAA
CCAATGATCCTCAGGAACTCGCACTGCGGCGCAACGAAGAGTACTGCCTG
CTGGACAGTTCTGAGATTCACTGGTGGAGAGTCCAGGACAGGAATGGGCA
TGAAGGATATGTACCAAGCAGTTATCTGGTGGAAAAATCTCCAAATAATC
TGGAAACCTATGAGTGGTACAATAAGAGTATCAGCCGAGACAAAGCTGAA
AAACTTCTTTTGGACACAGGCAAAGAAGGAGCCTTCATGGTAAGGGATTC
CAGGACTGCAGGAACATACACCGTGTCTGTTTTCACCAAGGCTGTTGTAA
GTGAGAACAATCCCTGTATAAAGCATTATCACATCAAGGAAACAAATGAC
AATCCTAAGCGATACTATGTGGCTGAAAAGTATGTGTTCGATTCCATCCC
TCTTCTCATCAACTATCACCAACATAATGGAGGAGGCCTGGTGACTCGAC
TCCGGTATCCAGTTTGTTTTGGGAGGCAGAAAGCCCCAGTTACAGCAGGG
CTGAGATACGGGAAATGGGTGATCGACCCCTCAGAGCTCACTTTTGTGCA
AGAGATTGGCAGTGGGCAATTTGGGTTGGTGCATCTGGGCTACTGGCTCA
ACAAGGACAAGGTGGCTATCAAAACCATTCGGGAAGGGGCTATGTCAGAA
GAGGACTTCATAGAGGAGGCTGAAGTAATGATGAAACTCTCTCATCCCAA
ACTGGTGCAGCTGTATGGGGTGTGCCTGGAGCAGGCCCCCATCTGCCTGG
TGTTTGAGTTCATGGAGCACGGCTGCCTGTCAGATTATCTACGCACCCAG
CGGGGACTTTTTGCTGCAGAGACCCTGCTGGGCATGTGTCTGGATGTGTG
TGAGGGCATGGCCTACCTGGAAGAGGCATGTGTCATCCACAGAGACTTGG
CTGCCAGAAATTGTTTGGTGGGAGAAAACCAAGTCATCAAGGTGTCTGAC
TTTGGGATGACAAGGTTCGTTCTGGATGATCAGTACACCAGTTCCACAGG
CACCAAATTCCCGGTGAAGTGGGCATCCCCAGAGGTTTTCTCTTTCAGTC
GCTATAGCAGCAAGTCCGATGTGTGGTCATTTGGTGTGCTGATGTGGGAA
GTTTTCAGTGAAGGCAAAATCCCGTATGAAAACCGAAGCAACTCAGAGGT
GGTGGAAGACATCAGTACCGGATTTCGGTTGTACAAGCCCCGGCTGGCCT
CCACACACGTCTACCAGATTATGAATCACTGCTGGAAAGAGAGACCAGAA
GATCGGCCAGCCTTCTCCAGACTGCTGCGTCAACTGGCTGAAATTGCAGA
ATCAGGACTTTAGGAATTCGCAAGGGCGAATTCTGCAGATATCCATCACACTGGCGGCCG

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMNNFILLEEQLIKKSQQKRRTS
PSNFKVRFFVLTKASLAYFEDRHGKKRTLKGSIELSRIKCVEIVKSDISI
PCHYKYPFQVVHDNYLLYVFAPDRESRQRWVLALKEETRNNNSLVPKYHP
NFWMDGKWRCCSQLEKLATGCAQYDPTKNASKKPLPPTPEDNRRPLWEPE
ETVVIALYDYQTNDPQELALRRNEEYCLLDSSEIHWWRVQDRNGHEGYVP
SSYLVEKSPNNLETYEWYNKSISRDKAEKLLLDTGKEGAFMVRDSRTAGT
YTVSVFTKAVVSENNPCIKHYHIKETNDNPKRYYVAEKYVFDSIPLLINY
HQHNGGGLVTRLRYPVCFGRQKAPVTAGLRYGKWVIDPSELTFVQEIGSG
QFGLVHLGYWLNKDKVAIKTIREGAMSEEDFIEEAEVMMKLSHPKLVQLY
GVCLEQAPICLVFEFMEHGCLSDYLRTQRGLFAAETLLGMCLDVCEGMAY
LEEACVIHRDLAARNCLVGENQVIKVSDFGMTRFVLDDQYTSSTGTKFPV
KWASPEVFSFSRYSSKSDVWSFGVLMWEVFSEGKIPYENRSNSEVVEDIS
TGFRLYKPRLASTHVYQIMNHCWKERPEDRPAFSRLLRQLAEIAESGL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5902
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; ; Estimated MW of expressed protein : 75200 Daltons