cDNA Clone PTIP

• Plasmid: pGEX6P1-hPTIP 590-800
• Parent Plasmid: pGEX6P1
• Expressed: GST-C3-PTIP 590-800
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCCAGCTTTTTGGACATGATCCAGCAGTGGAGATTCCAGAAGAAGGCTTCTTATTGGGATGTGTGTTTGCAATTGCGGATTATCCAGAGCAGATGTCTGATAAGCAACTGCTGGCCACCTGGAAAAGGATAATCCAGGCACATGGCGGCACTGTTGACCCCACCTTCACGAGTCGATGCACGCACCTTCTCTGTGAGAGTCAAGTCAGCAGCGCGTATGCACAGGCAATAAGAGAAAGAAAGAGATGTGTTACTGCACACTGGTTAAACACAGTCTTAAAGAAGAAGAAAATGGTACCGCCG
CACCGAGCCCTTCACTTCCCAGTGGCCTTCCCACCAGGAGGAAAGCCATG
TTCACAGCATATTATTTCTGTGACTGGATTTGTTGATAGTGACAGAGATG
ACCTAAAATTAATGGCTTATTTGGCAGGTGCCAAATATACGGGTTATCTA
TGCCGCAGCAACACAGTCCTCATCTGTAAAGAACCAACTGGTTTAAAGTA
TGAAAAAGCCAAAGAGTGGAGGATACCCTGTGTCAACGCCCAGTGGCTTG
GCGACATTCTTCTGGGAAACTTTGAGGCACTGAGGCAGATTCAGTATAGT
CGCTACACGGCATTCAGTCTGCAGGATCCATTTTGACCTACCCAGCATTT
AGTTTTAAATCTTTTAGATGCTTGGAGAGTTCCCTTAAAAGTGTCTGCAG
AGTTGTTGATGAGTATAAGACTACCTCCCAAACTGAAACAGAATGAAGTA
GCTAATGTCCAGCCTTCTTCCAAAAGAGCCAGAATTGAAGACGTACCACC
TCCCACTAAAAAGCTAACTCCAGAATTGACCCCTTTTGTGCTTTTCACTG
GATTCGAGCCTGTCCAGGTTCAACAGTATATTAAGAAGCTCTACATTCTT
GGTGGAGAGGTTGCGGAGTCTGCACAGAAGTGCACACACCTCATTGCCAG
CAAGGTGACTCGCACCGTGAAGTTCCTGACGGCGATTTCTGTCGTGAAGC
ACATAGTGACGCCAGAGTGGCTGGAAGAATGCTTCAGGTGTCAGAAGTTC
ATTGATGAGCAGAACTACATTCTCCGAGATGCTGAGGCAGAAGTACTTTT
CTCTTTCAGCTTGGAAGAATCCTTAAAACGGGCACACGTTTCTCCACTCT
TTAAGGCAAAATATTTTTACATCACACCTGGAATCTGCCCAAGTCTTTCC
ACTATGAAGGCAATCGTAGAGTGTGCAGGAGGAAAGGTGTTATCCAAGCA
GCCATCTTTCCGGAAGCTCATGGAGCACAAGCAGAACTCGAGTTTGTCGG
AAATAATTTTAATATCCTGTGAAAATGACCTTCATTTATGCCGAGAATAT
TTTGCCAGAGGCATAGATGTTCACAATGCAGAGTTCGTTCTGACTGGAGT
GCTCACTCAAACGCTGGACTATGAATCATATAAGTTTAACTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLEFQLFGHDPAVEIPEEGFLLG
CVFAIADYPEQMSDKQLLATWKRIIQAHGGTVDPTFTSRCTHLLCESQVS
SAYAQAIRERKRCVTAHWLNTVLKKKKMVPPHRALHFPVAFPPGGKPCSQ
HIISVTGFVDSDRDDLKLMAYLAGAKYTGYLCRSNTVLICKEPTGLKYEK
AKEWRIPCVNAQWLGDILLGNFEALRQIQYSRYTAFSLQDPF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5900
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 50759 Daltons