cDNA Clone YES1

• Plasmid: pFastBac.HTc YES1
• Parent Plasmid: pFastbac HTc
• Expressed: 6His-YES1
• Genbank: NM_005433
• Tag: 6His
• Species: Human

Sequence of Insert

GTCGACATGGGCTGCATTAAAAGTAAAGAAAACAAAAGTCCAGCCATTAAATACAGACCTGAAAATACTCCAGAGCCTGTCAGTACAAGTGTGAGCCATTATGGAGCAGAACCCACTACAGTGTCACCATGTCCGTCATCTTCAGCAAAGGGAACAGCAGTTAATTTCAGCAGTCTTTCCATGACACCATTTGGAGGATCCTCAGGGGTAACGCCTTTTGGAGGTGCATCTTCCTCATTTTCAGTGGTGCCAAGTTCATATCCTGCTGGTTTAACAGGTGGTGTTACTATATTTGTGGCCTTATAT
GATTATGAAGCTAGAACTACAGAAGACCTTTCATTTAAGAAGGGTGAAAG
ATTTCAAATAATTAACAATACGGAAGGAGATTGGTGGGAAGCAAGATCAA
TCGCTACAGGAAAGAATGGTTATATCCCGAGCAATTATGTAGCGCCTGCA
GATTCCATTCAGGCAGAAGAATGGTATTTTGGCAAAATGGGGAGAAAAGA
TGCTGAAAGATTACTTTTGAATCCTGGAAATCAACGAGGTATTTTCTTAG
TAAGAGAGAGTGAAACAACTAAAGGTGCTTATTCCCTTTCTATTCGTGAT
TGGGATGAGATAAGGGGTGACAATGTGAAACACTACAAAATTAGGAAACT
TGACAATGGTGGATACTATATCACAACCAGAGCACAATTTGATACTCTGC
AGAAATTGGTGAAACACTACACAGAACATGCTGATGGTTTATGCCACAAG
TTGACAACTGTGTGTCCAACTGTGAAACCTCAGACTCAAGGTCTAGCAAA
AGATGCTTGGGAAATCCCTCGAGAATCTTTGCGACTAGAGGTTAAACTAG
GACAAGGATGTTTCGGCGAAGTGTGGATGGGAACATGGAATGGAACCACG
AAAGTAGCAATCAAAACACTAAAACCAGGTACAATGATGCCAGAAGCTTT
CCTTCAAGAAGCTCAGATAATGAAAAAATTAAGACATGATAAACTTGTTC
CACTATATGCTGTTGTTTCTGAAGAACCAATTTACATTGTCACTGAATTT
ATGTCAAAAGGAAGCTTATTAGATTTCCTTAAGGAAGGAGATGGAAAGTA
TTTGAAGCTTCCACAGCTGGTTGATATGGCTGCTCAGATTGCTGATGGTA
TGGCATATATTGAAAGAATGAACTATATTCACCGAGATCTTCGGGCTGCT
AATATTCTTGTAGGAGAAAATCTTGTGTGCAAAATAGCAGACTTTGGTTT
AGCAAGGTTAATTGAAGACAATGAATACACAGCAAGACAAGGTGCAAAAT
TTCCAATCAAATGGACAGCTCCTGAAGCTGCACTGTATGGTCGGTTTACA
ATAAAGTCTGATGTCTGGTCATTTGGAATTCTGCAAACAGAACTAGTAAC
AAAGGGCCGAGTGCCATATCCAGGTATGGTGAACCGTGAAGTACTAGAAC
AAGTGGAGCGAGGATACAGGATGCCGTGCCCTCAGGGCTGTCCAGAATCC
CTCCATGAATTGATGAATCTGTGTTGGAAGAAGGACCCTGATGAAAGACC
AACATTTGAATATATTCAGTCCTTCTTGGAAGACTACTTCACTGCTACAG
AGCCACAGTACCAGCCAGGAGAAAATTTATAAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGIRNSKAYVDMGCIKSKENKSPAI
KYRPENTPEPVSTSVSHYGAEPTTVSPCPSSSAKGTAVNFSSLSMTPFGG
SSGVTPFGGASSSFSVVPSSYPAGLTGGVTIFVALYDYEARTTEDLSFKK
GERFQIINNTEGDWWEARSIATGKNGYIPSNYVAPADSIQAEEWYFGKMG
RKDAERLLLNPGNQRGIFLVRESETTKGAYSLSIRDWDEIRGDNVKHYKI
RKLDNGGYYITTRAQFDTLQKLVKHYTEHADGLCHKLTTVCPTVKPQTQG
LAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTKVAIKTLKPGTMMP
EAFLQEAQIMKKLRHDKLVPLYAVVSEEPIYIVTEFMSKGSLLDFLKEGD
GKYLKLPQLVDMAAQIADGMAYIERMNYIHRDLRAANILVGENLVCKIAD
FGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILQTE
LVTKGRVPYPGMVNREVLEQVERGYRMPCPQGCPESLHELMNLCWKKDPD
ERPTFEYIQSFLEDYFTATEPQYQPGENL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5884
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; ; Estimated MW of expressed protein : 65130 Daltons