cDNA Clone ZAP70

• Plasmid: pFastBac.HTb ZAP70
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-ZAP70
• Genbank: NM_001079
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGCCAGACCCCGCGGCGCACCTGCCCTTCTTCTACGGCAGCATCTCGCGTGCCGAGGCCGAGGAGCACCTGAAGCTGGCGGGCATGGCGGACGGGCTCTTCCTGCTGCGCCAGTGCCTGCGCTCGCTGGGCGGCTATGTGCTGTCGCTCGTGCACGATGTGCGCTTCCACCACTTTCCCATCGAGCGCCAGCTCAACGGCACCTACGCCATTGCCGGCGGCAAAGCGCACTGTGGACCGGCAGAGCTCTGCGAGTTCTACTCGCGCGACCCCGACGGGCTGCCCTGCAACCTGCGCAAG
CCGTGCAACCGGCCGTCGGGCCTCGAGCCGCAGCCGGGGGTCTTCGACTG
CCTGCGAGACGCCATGGTGCGTGACTACGTGCGCCAGACGTGGAAGCTGG
AGGGCGAGGCCCTGGAGCAGGCCATCATCAGCCAGGCCCCGCAGGTGGAG
AAGCTCATTGCTACGACGGCCCACGAGCGGATGCCCTGGTACCACAGCAG
CCTGACGCGTGAGGAGGCCGAGCGCAAACTTTACTCTGGGGCGCAGACCG
ACGGCAAGTTCCTGCTGAGGCCGCGGAAGGAGCAGGGCACATACGCCCTG
TCCCTCATCTATGGGAAGACGGTGTACCACTACCTCATCAGCCAAGACAA
GGCGGGCAAGTACTGCATTCCCGAGGGCACCAAGTTTGACACGCTCTGGC
AGCTGGTGGAGTATCTGAAGCTGAAGGCGGACGGGCTCATCTACTGCCTG
AAGGAGGCCTGCCCCAACAGCAGTGCCAGCAACGCCTCAGGGGCTGCTGC
TCCCACACTCCCAGCCCACCCATCCACGTTGACTCATCCTCAGAGACGAA
TCGACACCCTCAACTCAGATGGATACACCCCTGAGCCAGCACGCATAACG
TCCCCAGACAAACCGCGGCCGATGCCCATGGACACGAGCGTGTATGAGAG
CCCCTACAGCGACCCAGAGGAGCTCAAGGACAAGAAGCTCTTCCTGAAGC
GCGATAACCTCCTCATAGCTGACATTGAACTTGGCTGCGGCAACTTTGGC
TCAGTGCGCCAGGGCGTGTACCGCATGCGCAAGAAGCAGATCGACGTGGC
CATCAAGGTGCTGAAGCAGGGCACGGAGAAGGCAGACACGGAAGAGATGA
TGCGCGAGGCGCAGATCATGCACCAGCTGGACAACCCCTACATCGTGCGG
CTCATTGGCGTCTGCCAGGCCGAGGCCCTCATGCTGGTCATGGAGATGGC
TGGGGGCGGGCCGCTGCACAAGTTCCTGGTCGGCAAGAGGGAGGAGATCC
CTGTGAGCAATGTGGCCGAGCTGCTGCACCAGGTGTCCATGGGGATGAAG
TACCTGGAGGAGAAGAACTTTGTGCACCGTGACCTGGCGGCCCGCAACGT
CCTGCTGGTTAACCGGCACTACGCCAAGATCAGCGACTTTGGCCTCTCCA
AAGCACTGGGTGCCGACGACAGCTACTACACTGCCCGCTCAGCAGGGAAG
TGGCCGCTCAAGTGGTACGCACCCGAATGCATCAACTTCCGCAAGTTCTC
CAGCCGCAGCGATGTCTGGAGCTATGGGGTCACCATGTGGGAGGCCTTGT
CCTACGGCCAGAAGCCCTACAAGAAGATGAAAGGGCCGGAGGTCATGGCC
TTCATCGAGCAGGGCAAGCGGATGGAATGCCCACCAGAGTGTCCACCCGA
ACTGTACGCACTCATGAGTGACTGCTGGATCTACAAGTGGGAGGATCGCC
CCGACTTCCTGACCGTGGAGCAGCGCATGCGAGCCTGTTACTACAGCCTG
GCCAGCAAGGTGGAAGGGCCCCCAGGCAGCACACAGAAGGCTGAGGCTGC
CTGTGCCTGAGAATTCCCGGGTCGACTCGAGCGGCCG

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMPDPAAHLPFFYGSISRAEAEE
HLKLAGMADGLFLLRQCLRSLGGYVLSLVHDVRFHHFPIERQLNGTYAIA
GGKAHCGPAELCEFYSRDPDGLPCNLRKPCNRPSGLEPQPGVFDCLRDAM
VRDYVRQTWKLEGEALEQAIISQAPQVEKLIATTAHERMPWYHSSLTREE
AERKLYSGAQTDGKFLLRPRKEQGTYALSLIYGKTVYHYLISQDKAGKYC
IPEGTKFDTLWQLVEYLKLKADGLIYCLKEACPNSSASNASGAAAPTLPA
HPSTLTHPQRRIDTLNSDGYTPEPARITSPDKPRPMPMDTSVYESPYSDP
EELKDKKLFLKRDNLLIADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLK
QGTEKADTEEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPL
HKFLVGKREEIPVSNVAELLHQVSMGMKYLEEKNFVHRDLAARNVLLVNR
HYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDV
WSYGVTMWEALSYGQKPYKKMKGPEVMAFIEQGKRMECPPECPPELYALM
SDCWIYKWEDRPDFLTVEQRMRACYYSLASKVEGPPGSTQKAEAACA*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5882
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; Sequence difference compared with NM_001079: g1677a (silent in E559)