cDNA Clone ACTN4

• Plasmid: pEBG6P-1 ACTN4
• Parent Plasmid: pEBG6P
• Expressed: GST-ACTN4
• Genbank: NM_004924
• Tag: GST
• Species: Human

Sequence of Insert

gcggccgcgatggtggactaccacgcggcgaaccagtcgtaccagtacggccccagcagcgcgggcaatggcgctggcggcgggggcagcatgggcgactacatggcccaggaggacgactgggaccgggacctgctgctggacccggcctgggagaagcagcagcgcaagaccttcacggcatggtgcaactcccacctgcggaaggcaggcacacagatcgagaacattgatgaggacttccgagacgggctcaagctcatgctgctcctggaggtcatatcaggggagcggttacctaagccggagcgggggaagatgagagtgcacaaaatcaacaatgtgaacaaagcgctggactttattgccagcaaaggcgtcaagctggtctccatcggggcagaagagattgtggacggcaacgcaaagatgaccctgggaatgatctggaccatcatc
cttaggttcgccatccaggacatctccgtggaagagacctcggccaagga
agggctccttctctggtgccagagaaagacagccccgtataagaacgtca
atgtgcagaacttccacatcagctggaaggatggtcttgccttcaatgcc
ctgatccaccggcacagaccagagctgattgagtatgacaagctgaggaa
ggacgaccctgtcaccaacctgaacaatgccttcgaagtggctgagaaat
acctcgacatccccaagatgctggatgcagaggacatcgtgaacacggcc
cggcccgacgagaaggccataatgacctatgtgtccagcttctaccatgc
cttttcaggagcgcagaaggctgaaactgccgccaaccggatctgtaagg
tgctggctgtcaaccaagagaacgagcacctgatggaggactacgagaag
ctggccagcgacctcctggagtggatccggcgcaccatcccctggctgga
ggaccgtgtgccccaaaagactatccaggagatgcagcagaagctggagg
acttccgcgactaccggcgtgtgcacaagccgcccaaggtgcaggagaag
tgccagctggagatcaacttcaacacgctgcagaccaagctgcgcctcag
caaccggcccgccttcatgccctccgagggcaagatggtctcggacatca
acaatggctggcagcacttggagcaggctgagaagggctacgaggagtgg
ctgctgaatgagatccgcaggctggagcggctcgaccacctggcagagaa
gttccggcagaaggcctccatccacgaggcctggactgacgggaaggaag
ccatgctgaagcaccgggactacgagacggccacactatcggacatcaaa
gccctcattcgcaagcacgaggccttcgagagcgacctggctgcgcacca
ggaccgcgtggagcagatcgccgccattgcccaggagctcaacgagctgg
attactacgactcccacaatgtcaacacccggtgccagaagatctgtgac
cagtgggacgccctcggctctctgacacatagtcgcagggaagccctgga
gaaaacagagaagcagctggaggccatcgaccagctgcacctggaatacg
ccaagcgcgcggcccccttcaacaactggatggagagcgccatggaggac
ctccaggacatgttcatcgtccataccatcgaggagattgagggcctgat
ctcagcccatgaccagttcaagtccaccctgccggacgccgatagggagc
gcgaggccatcctggccatccacaaggaggcccagaggatcgctgagagc
aaccacatcaagctgtcgggcagcaacccctacaccaccgtcaccccgca
aatcatcaactccaagtgggagaaggtgcagcagctggtgccaaaacggg
accatgccctcctggaggagcagagcaagcagcagtccaacgagcacctg
cgccgccagttcgccagccaggccaatgttgtggggccctggatccagac
caagatggaggagatcgggcgcatctccattgagatgaacgggaccctgg
aggaccagctgagccacctgaagcagtatgaacgcagcatcgtggactac
aagcccaacctggacctgctggagcagcagcaccagctcatccaggaggc
cctcatcttcgacaacaagcacaccaactataccatggagcacatccgcg
tgggctgggagcagctgctcaccaccattgcccgcaccatcaacgaggtg
gagaaccagatcctcacccgcgacgccaagggcatcagccaggagcagat
gcaggagttccgggcgtccttcaaccacttcgacaaggatcatggcgggg
cgctggggcccgaggagttcaaggcctgcctcatcagcctgggctacgac
gtggagaacgaccggcagggtgaggccgagttcaaccgcatcatgagcct
ggtcgaccccaaccatagcggccttgtgaccttccaagccttcatcgact
tcatgtcgcgggagaccaccgacacggacacggctgaccaggtcatcgct
tccttcaaggtcttagcaggggacaagaacttcatcacagctgaggagct
gcggagagagctgccccccgaccaggccgagtactgcatcgcccgcatgg
cgccataccagggccctgacgccgtgcccggtgccctcgactacaagtcc
ttctccacggccttgtatggcgagagcgacctgtgagcggccgc

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMVDY
HAANQSYQYGPSSAGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRK
TFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPERGKM
RVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFA
IQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHR
HRPELIEYDKLRKDDPVTNLNNAFEVAEKYLDIPKMLDAEDIVNTARPDE
KAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYEKLASD
LLEWIRRTIPWLEDRVPQKTIQEMQQKLEDFRDYRRVHKPPKVQEKCQLE
INFNTLQTKLRLSNRPAFMPSEGKMVSDINNGWQHLEQAEKGYEEWLLNE
IRRLERLDHLAEKFRQKASIHEAWTDGKEAMLKHRDYETATLSDIKALIR
KHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSHNVNTRCQKICDQWDA
LGSLTHSRREALEKTEKQLEAIDQLHLEYAKRAAPFNNWMESAMEDLQDM
FIVHTIEEIEGLISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIK
LSGSNPYTTVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQF
ASQANVVGPWIQTKMEEIGRISIEMNGTLEDQLSHLKQYERSIVDYKPNL
DLLEQQHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQI
LTRDAKGISQEQMQEFRASFNHFDKDHGGALGPEEFKACLISLGYDVEND
RQGEAEFNRIMSLVDPNHSGLVTFQAFIDFMSRETTDTDTADQVIASFKV
LAGDKNFITAEELRRELPPDQAEYCIARMAPYQGPDAVPGALDYKSFSTA
LYGESDL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5871
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express ACTN4 with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 133054 Daltons