cDNA Clone ITK

• Plasmid: pGEX6P-1-ITK
• Parent Plasmid: pGEX6P1
• Expressed: GST-ITK
• Genbank: NM_005546
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGAACAACTTTATCCTCCTGGAAGAACAGCTCATCAAGAAATCCCAACAAAAGAGAAGAACTTCTCCCTCGAACTTTAAAGTCCGCTTCTTTGTGTTAACCAAAGCCAGCCTGGCATACTTTGAAGATCGTCATGGGAAGAAGCGCACGCTGAAGGGGTCCATTGAGCTCTCCCGAATCAAATGTGTTGAGATTGTGAAAAGTGACATCAGCATCCCATGCCACTATAAATACCCGTTTCAGGTGGTGCATGACAACTACCTCCTATATGTGTTTGCTCCAGATCGTGAGAGCCGGCAG
CGCTGGGTGCTGGCCCTTAAAGAAGAAACGAGGAATAATAACAGTTTGGT
GCCTAAATATCATCCTAATTTCTGGATGGATGGGAAGTGGAGGTGCTGTT
CTCAGCTGGAGAAGCTTGCAACAGGCTGTGCCCAATATGATCCAACCAAG
AATGCTTCAAAGAAGCCTCTTCCTCCTACTCCTGAAGACAACAGGCGACC
ACTTTGGGAACCTGAAGAAACTGTGGTCATTGCCTTATATGACTACCAAA
CCAATGATCCTCAGGAACTCGCACTGCGGCGCAACGAAGAGTACTGCCTG
CTGGACAGTTCTGAGATTCACTGGTGGAGAGTCCAGGACAGGAATGGGCA
TGAAGGATATGTACCAAGCAGTTATCTGGTGGAAAAATCTCCAAATAATC
TGGAAACCTATGAGTGGTACAATAAGAGTATCAGCCGAGACAAAGCTGAA
AAACTTCTTTTGGACACAGGCAAAGAAGGAGCCTTCATGGTAAGGGATTC
CAGGACTGCAGGAACATACACCGTGTCTGTTTTCACCAAGGCTGTTGTAA
GTGAGAACAATCCCTGTATAAAGCATTATCACATCAAGGAAACAAATGAC
AATCCTAAGCGATACTATGTGGCTGAAAAGTATGTGTTCGATTCCATCCC
TCTTCTCATCAACTATCACCAACATAATGGAGGAGGCCTGGTGACTCGAC
TCCGGTATCCAGTTTGTTTTGGGAGGCAGAAAGCCCCAGTTACAGCAGGG
CTGAGATACGGGAAATGGGTGATCGACCCCTCAGAGCTCACTTTTGTGCA
AGAGATTGGCAGTGGGCAATTTGGGTTGGTGCATCTGGGCTACTGGCTCA
ACAAGGACAAGGTGGCTATCAAAACCATTCGGGAAGGGGCTATGTCAGAA
GAGGACTTCATAGAGGAGGCTGAAGTAATGATGAAACTCTCTCATCCCAA
ACTGGTGCAGCTGTATGGGGTGTGCCTGGAGCAGGCCCCCATCTGCCTGG
TGTTTGAGTTCATGGAGCACGGCTGCCTGTCAGATTATCTACGCACCCAG
CGGGGACTTTTTGCTGCAGAGACCCTGCTGGGCATGTGTCTGGATGTGTG
TGAGGGCATGGCCTACCTGGAAGAGGCATGTGTCATCCACAGAGACTTGG
CTGCCAGAAATTGTTTGGTGGGAGAAAACCAAGTCATCAAGGTGTCTGAC
TTTGGGATGACAAGGTTCGTTCTGGATGATCAGTACACCAGTTCCACAGG
CACCAAATTCCCGGTGAAGTGGGCATCCCCAGAGGTTTTCTCTTTCAGTC
GCTATAGCAGCAAGTCCGATGTGTGGTCATTTGGTGTGCTGATGTGGGAA
GTTTTCAGTGAAGGCAAAATCCCGTATGAAAACCGAAGCAACTCAGAGGT
GGTGGAAGACATCAGTACCGGATTTCGGTTGTACAAGCCCCGGCTGGCCT
CCACACACGTCTACCAGATTATGAATCACTGCTGGAAAGAGAGACCAGAA
GATCGGCCAGCCTTCTCCAGACTGCTGCGTCAACTGGCTGAAATTGCAGA
ATCAGGACTTTAGGAATTCGCAAGGGCGAATTCTGCAGATATCCATCACACTGGCGGCCG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMNNFILLEEQLIKKSQQKR
RTSPSNFKVRFFVLTKASLAYFEDRHGKKRTLKGSIELSRIKCVEIVKSD
ISIPCHYKYPFQVVHDNYLLYVFAPDRESRQRWVLALKEETRNNNSLVPK
YHPNFWMDGKWRCCSQLEKLATGCAQYDPTKNASKKPLPPTPEDNRRPLW
EPEETVVIALYDYQTNDPQELALRRNEEYCLLDSSEIHWWRVQDRNGHEG
YVPSSYLVEKSPNNLETYEWYNKSISRDKAEKLLLDTGKEGAFMVRDSRT
AGTYTVSVFTKAVVSENNPCIKHYHIKETNDNPKRYYVAEKYVFDSIPLL
INYHQHNGGGLVTRLRYPVCFGRQKAPVTAGLRYGKWVIDPSELTFVQEI
GSGQFGLVHLGYWLNKDKVAIKTIREGAMSEEDFIEEAEVMMKLSHPKLV
QLYGVCLEQAPICLVFEFMEHGCLSDYLRTQRGLFAAETLLGMCLDVCEG
MAYLEEACVIHRDLAARNCLVGENQVIKVSDFGMTRFVLDDQYTSSTGTK
FPVKWASPEVFSFSRYSSKSDVWSFGVLMWEVFSEGKIPYENRSNSEVVE
DISTGFRLYKPRLASTHVYQIMNHCWKERPEDRPAFSRLLRQLAEIAESG
L*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5858
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Construct for bacterial expression of N-terminal GST-tagged ITK (Made by Luisa Luciani) ; ; Estimated MW of expressed protein : 98652 Daltons