cDNA Clone DCUN1D1

• Plasmid: pcDNA5-FRT/TO-HA- DCUN1D1 D211A A235R D241A
• Parent Plasmid: pcDNA5 FRT/TO HA1
• Expressed: HA-DCUN1D1 D211A A235R D241A
• Tag: HA
• Species: Human

Sequence of Insert

ggatccATGAACAAGTTGAAATCATCGCAGAAGGATAAAGTTCGTCAGTT
TATGATCTTCACACAATCTAGTGAAAAAACAGCAGTAAGTTGTCTTTCTC
AAAATGACTGGAAGTTAGATGTTGCAACAGATAATTTTTTCCAAAATCCT
GAACTTTATATACGAGAGAGTGTAAAAGGATCATTGGACAGGAAGAAGTT
AGAACAGCTGTACAATAGATACAAAGACCCTCAAGATGAGAATAAAATTG
GAATAGATGGCATACAGCAGTTCTGTGATGACCTGGCACTCGATCCAGCC
AGCATTAGTGTGTTGATTATTGCATGGAAGTTCAGAGCAGCAACACAGTG
CGAGTTCTCCAAACAGGAGTTCATGGATGGCATGACAGAATTAGGATGTG
ACAGCATAGAAAAACTAAAGGCCCAGATACCCAAGATGGAACAAGAATTG
AAAGAACCAGGACGATTTAAGGATTTTTACCAGTTTACTTTTAATTTTGC
AAAGAATCCAGGACAAAAAGGATTAGATCTAGAAATGGCCATTGCCTACT
GGAACTTAGTGCTTAATGGAAGATTTAAATTCTTAGACTTATGGAATAAA
TTTTTGTTGGAACATCATAAACGATCAATACCAAAAGcCACTTGGAATCT
TCTTTTAGACTTCAGTACGATGATTGCAGATGACATGTCTAATTATGATG
AAGAAGGAagATGGCCTGTTCTTATTGcTGACTTTGTGGAATTTGCACGC
CCTCAAATTGCTGGGACAAAAAGTACAACAGTGTAGGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMNKLKSSQKDKVRQFMIFTQSSEKTAVSCLSQNDWKLD
VATDNFFQNPELYIRESVKGSLDRKKLEQLYNRYKDPQDENKIGIDGIQQ
FCDDLALDPASISVLIIAWKFRAATQCEFSKQEFMDGMTELGCDSIEKLK
AQIPKMEQELKEPGRFKDFYQFTFNFAKNPGQKGLDLEMAIAYWNLVLNG
RFKFLDLWNKFLLEHHKRSIPKATWNLLLDFSTMIADDMSNYDEEGRWPV
LIADFVEFARPQIAGTKSTTV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU23570
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: a632c (D211A), g703a/c704g (A235R) and a722c (D241A). clone also harbours silent substitution g318a (Ala106) compared to NCBI ref