cDNA Clone Ku80

• Plasmid: pFBDual Ku80/6His.Ku70
• Parent Plasmid: pFastbacDual
• Expressed: Ku80/6His-Ku70
• Genbank: pFastBacDual Genbank protein 1 : J04611
• Tag: 6His
• Species: Human

Sequence of Insert

DNA sequence of insert 1 (6His.Ku70) :
CTCGAGAAACCATGCACCATCACCATCACCATATGTCAGGGTGGGAGTCA
TATTACAAAACCGAGGGCGATGAAGAAGCAGAGGAAGAACAAGAAGAGAA
CCTTGAAGCAAGTGGAGACTATAAATATTCAGGAAGAGATAGTTTGATTT
TTTTGGTTGATGCCTCCAAGGCTATGTTTGAATCTCAGAGTGAAGATGAG
TTGACACCTTTTGACATGAGCATCCAGTGTATCCAAAGTGTGTACATCAG
TAAGATCATAAGCAGTGATCGAGATCTCTTGGCTGTGGTGTTCTATGGCA
CCGAGAAAGACAAAAATTCAGTGAATTTTAAAAATATTTACGTCTTACAG
GAGCTGGATAATCCAGGTGCAAAACGAATTCTAGAGCTTGACCAGTTTAA
GGGGCAGCAGGGACAAAAACGTTTCCAAGACATGATGGGCCACGGATCTG
ACTACTCACTCAGTGAAGTGCTGTGGGTCTGTGCCAACCTCTTTAGTGAT
GTCCAATTCAAGATGAGTCATAAGAGGATCATGCTGTTCACCAATGAAGA
CAACCCCCATGGCAATGACAGTGCCAAAGCCAGCCGGGCCAGGACCAAAG
CCGGTGATCTCCGAGATACAGGCATCTTCCTTGACTTGATGCACCTGAAG
AAACCTGGGGGCTTTGACATATCCTTGTTCTACAGAGATATCATCAGCAT
AGCAGAGGATGAGGACCTCAGGGTTCACTTTGAGGAATCCAGCAAGCTAG
AAGACCTGTTGCGGAAGGTTCGCGCCAAGGAGACCAGGAAGCGAGCACTC
AGCAGGTTAAAGCTGAAGCTCAACAAAGATATAGTGATCTCTGTGGGCAT
TTATAATCTGGTCCAGAAGGCTCTCAAGCCTCCTCCAATAAAGCTCTATC
GGGAAACAAATGAACCAGTGAAAACCAAGACCCGGACCTTTAATACAAGT
ACAGGCGGTTTGCTTCTGCCTAGCGATACCAAGAGGTCTCAGATCTATGG
GAGTCGTCAGATTATACTGGAGAAAGAGGAAACAGAAGAGCTAAAACGGT
TTGATGATCCAGGTTTGATGCTCATGGGTTTCAAGCCGTTGGTACTGCTG
AAGAAACACCATTACCTGAGGCCCTCCCTGTTCGTGTACCCAGAGGAGTC
GCTGGTGATTGGGAGCTCAACCCTGTTCAGTGCTCTGCTCATCAAGTGTC
TGGAGAAGGAGGTTGCAGCATTGTGCAGATACACACCCCGCAGGAACATC
CCTCCTTATTTTGTGGCTTTGGTGCCACAGGAAGAAGAGTTGGATGACCA
GAAAATTCAGGTGACTCCTCCAGGCTTCCAGCTGGTCTTTTTACCCTTTG
CTGATGATAAAAGGAAGATGCCCTTTACTGAAAAAATCATGGCAACTCCA
GAGCAGGTGGGCAAGATGAAGGCTATCGTTGAGAAGCTTCGCTTCACATA
CAGAAGTGACAGCTTTGAGAACCCCGTGCTGCAGCAGCACTTCAGGAACC
TGGAGGCCTTGGCCTTGGATTTGATGGAGCCGGAACAAGCAGTGGACCTG
ACATTGCCCAAGGTTGAAGCAATGAATAAAAGACTGGGCTCCTTGGTGGA
TGAGTTTAAGGAGCTTGTTTACCCACCAGATTACAATCCTGAAGGGAAAG
TTACCAAGAGAAAACACGATAATGAAGGTTCTGGAAGCAAAAGGCCCAAG
GTGGAGTATTCAGAAGAGGAGCTGAAGACCCACATCAGCAAGGGTACGCT
GGGCAAGTTCACTGTGCCCATGCTGAAAGAGGCCTGCCGGGCTTACGGGC
TGAAGAGTGGTCTGAAGAAGCAGGAGCTGCTGGAAGCCCTCACCAAGCAC
TTCCAGGACTGAGGTACC

DNA sequence of insert 2 (Ku80) : GGATCCATGGTGCGGTCGGGGAATAAGGCAGCTGTTGTGCTGTGTATGGACGTGGGCTTTACCATGAGTAACTCCATTCCTGGTATAGAATCCCCATTTGAACAAGCAAAGAAGGTGATAACCATGTTTGTACAGCGACAGGTGTTTGCTGAGAACAAGGATGAGATTGCTTTAGTCCTGTTTGGTACAGATGGCACTGACAATCCCCTTTCTGGTGGGGATCAGTATCAGAACATCACAGTGCACAGACATCTGATGCTACCAGATTTTGATTTGCTGGAGGACATTGAAAGCAAAATCCAACCA
GGTTCTCAACAGGCTGACTTCCTGGATGCACTAATCGTGAGCATGGATGT
GATTCAACATGAAACAATAGGAAAGAAGTTTGAGAAGAGGCATATTGAAA
TATTCACTGACCTCAGCAGCCGATTCAGCAAAAGTCAGCTGGATATTATA
ATTCATAGCTTGAAGAAATGTGACATCTCCCTGCAATTCTTCTTGCCTTT
CTCACTTGGCAAGGAAGATGGAAGTGGGGACAGAGGAGATGGCCCCTTTC
GCTTAGGTGGCCATGGGCCTTCCTTTCCACTAAAAGGAATTACCGAACAG
CAAAAAGAAGGTCTTGAGATAGTGAAAATGGTGATGATATCTTTAGAAGG
TGAAGATGGGTTGGATGAAATTTATTCATTCAGTGAGAGTCTGAGAAAAC
TGTGCGTCTTCAAGAAAATTGAGAGGCATTCCATTCACTGGCCCTGCCGA
CTGACCATTGGCTCCAATTTGTCTATAAGGATTGCAGCCTATAAATCGAT
TCTACAGGAGAGAGTTAAAAAGACTTGGACAGTTGTGGATGCAAAAACCC
TAAAAAAAGAAGATATACAAAAAGAAACAGTTTATTGCTTAAATGATGAT
GATGAAACTGAAGTTTTAAAAGAGGATATTATTCAAGGGTTCCGCTATGG
AAGTGATATAGTTCCTTTCTCTAAAGTGGATGAGGAACAAATGAAATATA
AATCGGAGGGGAAGTGCTTCTCTGTTTTGGGATTTTGTAAATCTTCTCAG
GTTCAGAGAAGATTCTTCATGGGAAATCAAGTTCTAAAGGTCTTTGCAGC
AAGAGATGATGAGGCAGCTGCAGTTGCACTTTCCTCCCTGATTCATGCTT
TGGATGACTTAGACATGGTGGCCATAGTTCGATATGCTTATGACAAAAGA
GCTAATCCTCAAGTCGGCGTGGCTTTTCCTCATATCAAGCATAACTATGA
GTGTTTAGTGTATGTGCAGCTGCCTTTCATGGAAGACTTGCGGCAATACA
TGTTTTCATCCTTGAAAAACAGTAAGAAATATGCTCCCACCGAGGCACAG
TTGAATGCTGTTGATGCTTTGATTGACTCCATGAGCTTGGCAAAGAAAGA
TGAGAAGACAGACACCCTTGAAGACTTGTTTCCAACCACCAAAATCCCAA
ATCCTCGATTTCAGAGATTATTTCAGTGTCTGCTGCACAGAGCTTTACAT
CCCCGGGAGCCTCTACCCCCAATTCAGCAGCATATTTGGAATATGCTGAA
TCCTCCCGCTGAGGTGACAACGAAAAGTCAGATTCCTCTCTCTAAAATAA
AGACCCTTTTTCCTCTGATTGAAGCCAAGAAAAAGGATCAAGTGACTGCT
CAGGAAATTTTCCAAGACAACCATGAAGATGGACCTACAGCTAAAAAATT
AAAGACTGAGCAAGGGGGAGCCCACTTCAGCGTCTCCAGTCTGGCTGAAG
GCAGTGTCACCTCTGTTGGAAGTGTGAATCCTGCTGAAAACTTCCGTGTT
CTAGTGAAACAGAAGAAGGCCAGCTTTGAGGAAGCGAGTAACCAGCTCAT
AAATCACATCGAACAGTTTTTGGATACTAATGAAACACCGTATTTTATGA
AGAGCATAGACTGCATCCGAGCCTTCCGGGAAGAAGCCATTAAGTTTTCA
GAAGAGCAGCGCTTTAACAACTTCCTGAAAGCCCTTCAAGAGAAAGTGGA
AATTAAACAATTAAATCATTTCTGGGAAATTGTTGTCCAGGATGGAATTA
CTCTGATCACCAAAGAGGAAGCCTCTGGAAGTTCTGTCACAGCTGAGGAA
GCCAAAAAGTTTCTGGCCCCCAAAGACAAACCAAGTGGAGACACAGCAGC
TGTATTTGAAGAAGGTGGTGATGTGGACGATTTATTGGACATGATATAGGGATCC

Amino Acid Sequence

Amino acid sequence of expressed protein 1 (p10 promoter) (6His.Ku70) :MHHHHHHMSGWESYYKTEGDEEAEEEQEENLEASGDYKYSGRDSLIFLVD
ASKAMFESQSEDELTPFDMSIQCIQSVYISKIISSDRDLLAVVFYGTEKD
KNSVNFKNIYVLQELDNPGAKRILELDQFKGQQGQKRFQDMMGHGSDYSL
SEVLWVCANLFSDVQFKMSHKRIMLFTNEDNPHGNDSAKASRARTKAGDL
RDTGIFLDLMHLKKPGGFDISLFYRDIISIAEDEDLRVHFEESSKLEDLL
RKVRAKETRKRALSRLKLKLNKDIVISVGIYNLVQKALKPPPIKLYRETN
EPVKTKTRTFNTSTGGLLLPSDTKRSQIYGSRQIILEKEETEELKRFDDP
GLMLMGFKPLVLLKKHHYLRPSLFVYPEESLVIGSSTLFSALLIKCLEKE
VAALCRYTPRRNIPPYFVALVPQEEELDDQKIQVTPPGFQLVFLPFADDK
RKMPFTEKIMATPEQVGKMKAIVEKLRFTYRSDSFENPVLQQHFRNLEAL
ALDLMEPEQAVDLTLPKVEAMNKRLGSLVDEFKELVYPPDYNPEGKVTKR
KHDNEGSGSKRPKVEYSEEELKTHISKGTLGKFTVPMLKEACRAYGLKSG
LKKQELLEALTKHFQD*

Amino acid sequence of expressed protein 2(Polyhedrin promoter)(Ku80) :MVRSGNKAAVVLCMDVGFTMSNSIPGIESPFEQAKKVITMFVQRQVFAEN
KDEIALVLFGTDGTDNPLSGGDQYQNITVHRHLMLPDFDLLEDIESKIQP
GSQQADFLDALIVSMDVIQHETIGKKFEKRHIEIFTDLSSRFSKSQLDII
IHSLKKCDISLQFFLPFSLGKEDGSGDRGDGPFRLGGHGPSFPLKGITEQ
QKEGLEIVKMVMISLEGEDGLDEIYSFSESLRKLCVFKKIERHSIHWPCR
LTIGSNLSIRIAAYKSILQERVKKTWTVVDAKTLKKEDIQKETVYCLNDD
DETEVLKEDIIQGFRYGSDIVPFSKVDEEQMKYKSEGKCFSVLGFCKSSQ
VQRRFFMGNQVLKVFAARDDEAAAVALSSLIHALDDLDMVAIVRYAYDKR
ANPQVGVAFPHIKHNYECLVYVQLPFMEDLRQYMFSSLKNSKKYAPTEAQ
LNAVDALIDSMSLAKKDEKTDTLEDLFPTTKIPNPRFQRLFQCLLHRALH
PREPLPPIQQHIWNMLNPPAEVTTKSQIPLSKIKTLFPLIEAKKKDQVTA
QEIFQDNHEDGPTAKKLKTEQGGAHFSVSSLAEGSVTSVGSVNPAENFRV
LVKQKKASFEEASNQLINHIEQFLDTNETPYFMKSIDCIRAFREEAIKFS
EEQRFNNFLKALQEKVEIKQLNHFWEIVVQDGITLITKEEASGSSVTAEE
AKKFLAPKDKPSGDTAAVFEEGGDVDDLLDMI*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5778
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus dual expression plasmid to express Ku80 and 6His-tagged Ku70.