cDNA Clone ERK5

• Synonyms: MAPK7
• Plasmid: pVAh-CD2-FLAG-ERK5
• Parent Plasmid: pVAhCD2-Age1
• Expressed: FLAG-ERK5
• Genbank: NM_002749
• Tag: FLAG
• Species: Human

Sequence of Insert

accggtgccaccatgGACTACAAGGACGACGATGACAAGatggccgagcctctgaaggaggaagacggcgaggacggctctgcggagccccccgggcccgtgaaggccgaacccgcccacaccgctgcctctgtagcggccaagaacctggccctgcttaaagcccgctccttcgatgtgacctttgacgtgggcgacgagtacgagatcatcgagaccataggcaacggggcctatggagtggtgtcctccgcccgccgccgcctcaccggccagcaggtggccatcaagaagatccctaatgctttcgatgtggtgaccaatgccaagcggaccctcagggagctgaagatcctcaagcactttaaacacgacaacatcatcgccatcaaggacatcctgaggcccaccgtgccctatggcgaattcaaatctgtctacgtggtcctggacctgatggaaagcgacctgcaccagatcatccactcctcacagcccctcacactggaacacgtgcgctacttcctgtaccaactgctgcggggcctgaagtacatgcactcggctcaggtcatccaccgtgacctgaagccctccaacctattggtgaatgagaactgtgagctcaagattggtgactttggtatggctcgtggcctgtgcacctcgcccgctgaacatcagtacttcatgactgagtatgtggccacgcgctggtaccgtgcgcccgagctcatgctctctttgcatgagtatacacaggctattgacctctggtctgtgggctgcatctttggtgagatgctggcccggcgccagctcttcccaggcaaaaactatgtacaccagctacagctcatcatgatggtgctgggtaccccatcaccagccgtgattcaggctgtgggggctgagagggtgcgggcctatatccagagcttgccaccacgccagcctgtgccctgggagacagtgtacccaggtgccgaccgccaggccctatcactgctgggtcgcatgctgcgttttgagcccagcgctcgcatctcagcagctgctgcccttcgccaccctttcctggccaagtaccatgatcctgatgatgagcctgactgtgccccgccctttgactttgcctttgaccgcgaagccctcactcgggagcgcattaaggaggccattgtggctgaaattgaggacttccatgcaaggcgtgagggcatccgccaacagatccgcttccagccttctctacagcctgtggctagtgagcctggctgtccagatgttgaaatgcccagtccctgggctcccagtggggactgtgccatggagtctccaccaccagccccgccaccatgccccggccctgcacctgacaccattgatctgaccctgcagccacctccaccagtcagtgagcctgccccaccaaagaaagatggtgccatctcagacaatactaaggctgcccttaaagctgccctgctcaagtctttgaggagccggctcagagatggccccagcgcacccctggaggctcctgagcctcggaagccggtgacagcccaggagcgccagcgggagcgggaggagaagcggcggaggcggcaagaacgagccaaggagcgggagaaacggcggcaggagcgggagcgaaaggaacggggggctggggcctctgggggcccctccactgaccccttggctggactagtgctcagtgacaatgacagaagcctgttggaacgctggactcgaatggcccggcccgcagccccagccctcacctctgtgccggcccctgccccagcgccaacgccaaccccaaccccagtccaacctaccagtcctcctcctggccctgtagcccagcccactggcccgcaaccacaatctgcgggctctacctctggccctgtaccccagcctgcctgcccaccccctggccctgcaccccaccccactggccctcctgggcccatccct
gtccccgcgccaccccagattgccacctccaccagcctcctggctgccca
gtcacttgtgccaccccctgggctgcctggctccagcaccccaggagttt
tgccttacttcccacctggcctgccgcccccagacgccgggggagcccct
cagtcttccatgtcagagtcacctgatgtcaaccttgtgacccagcagct
atctaagtcacaggtggaggaccccctgccccctgtgttctcaggcacac
caaagggcagtggggctggctacggtgttggctttgacctggaggaattc
ttaaaccagtctttcgacatgggcgtggctgatgggccacaggatggcca
ggcagattcagcctctctctcagcctccctgcttgctgactggctcgaag
gccatggcatgaaccctgccgatattgagtccctgcagcgtgagatccag
atggactccccaatgctgctggctgacctgcctgacctccaggacccctg
aaccggt

Amino Acid Sequence

MDYKDDDDKMAEPLKEEDGEDGSAEPPGPVKAEPAHTAASVAAKNLALLK
ARSFDVTFDVGDEYEIIETIGNGAYGVVSSARRRLTGQQVAIKKIPNAFD
VVTNAKRTLRELKILKHFKHDNIIAIKDILRPTVPYGEFKSVYVVLDLME
SDLHQIIHSSQPLTLEHVRYFLYQLLRGLKYMHSAQVIHRDLKPSNLLVN
ENCELKIGDFGMARGLCTSPAEHQYFMTEYVATRWYRAPELMLSLHEYTQ
AIDLWSVGCIFGEMLARRQLFPGKNYVHQLQLIMMVLGTPSPAVIQAVGA
ERVRAYIQSLPPRQPVPWETVYPGADRQALSLLGRMLRFEPSARISAAAA
LRHPFLAKYHDPDDEPDCAPPFDFAFDREALTRERIKEAIVAEIEDFHAR
REGIRQQIRFQPSLQPVASEPGCPDVEMPSPWAPSGDCAMESPPPAPPPC
PGPAPDTIDLTLQPPPPVSEPAPPKKDGAISDNTKAALKAALLKSLRSRL
RDGPSAPLEAPEPRKPVTAQERQREREEKRRRRQERAKEREKRRQERERK
ERGAGASGGPSTDPLAGLVLSDNDRSLLERWTRMARPAAPALTSVPAPAP
APTPTPTPVQPTSPPPGPVAQPTGPQPQSAGSTSGPVPQPACPPPGPAPH
PTGPPGPIPVPAPPQIATSTSLLAAQSLVPPPGLPGSSTPGVLPYFPPGL
PPPDAGGAPQSSMSESPDVNLVTQQLSKSQVEDPLPPVFSGTPKGSGAGY
GVGFDLEEFLNQSFDMGVADGPQDGQADSASLSASLLADWLEGHGMNPAD
IESLQREIQMDSPMLLADLPDLQDP*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU5766
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Construct for expression of FLAG.ERK5 in mouse T-cells. Parental vector from D. Cantrell.