cDNA Clone E1a

• Plasmid: pCMV-HA E1a 42-end
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-E1a 42-end
• Genbank: P22314/X56976
• Tag: HA
• Species: Human

Sequence of Insert

ggatccGCCAAGAACGGCAGTGAAGCAGACATAGACGAGGGCCTTTACTCCCGGCAGCTGTATGTGTTGGGCCATGAGGCAATGAAGCGGCTCCAGACATCCAGTGTCCTGGTATCAGGCCTGCGGGGCCTGGGCGTGGAGATCGCTAAGAACATCATCCTTGGTGGGGTCAAGGCTGTTACCCTACATGACCAGGGCACTGCCCAGTGGGCTGATCTTTCCTCCCAGTTCTACCTGCGGGAGGAGGACATCGGTAAAAACCGGGCCGAGGTATCACAGCCCCGCCTCGCTGAGCTCAACAGCTAT
GTGCCTGTCACTGCCTACACTGGACCCCTCGTTGAGGACTTCCTTAGTGG
TTTCCAGGTGGTGGTGCTCACCAACACCCCCCTGGAGGACCAGCTGCGAG
TGGGTGAGTTCTGTCACAACCGTGGCATCAAGCTGGTGGTGGCAGACACG
CGGGGCCTGTTTGGGCAGCTCTTCTGTGACTTTGGAGAGGAAATGATCCT
CACAGATTCCAATGGGGAGCAGCCACTCAGTGCTATGGTTTCTATGGTTA
CCAAGGACAACCCCGGTGTGGTTACCTGCCTGGATGAGGCCCGACACGGG
TTTGAGAGCGGGGACTTTGTCTCCTTTTCAGAAGTACAGGGCATGGTTGA
ACTCAACGGAAATCAGCCCATGGAGATCAAAGTCCTGGGTCCTTATACCT
TTAGCATCTGTGACACCTCCAACTTCTCCGACTACATCCGTGGAGGCATC
GTCAGTCAGGTCAAAGTACCTAAGAAGATTAGCTTTAAATCCTTGGTGGC
CTCACTGGCAGAACCTGACTTTGTGGTGACGGACTTCGCCAAGTTTTCTC
GCCCTGCCCAGCTGCACATTGGCTTCCAGGCCCTGCACCAGTTCTGTGCT
CAGCATGGCCGGCCACCTCGGCCCCGCAATGAGGAGGATGCAGCAGAACT
GGTAGCCTTAGCACAGGCTGTGAATGCTCGAGCCCTGCCAGCAGTGCAGC
AAAATAACCTGGACGAGGACCTCATCCGGAAGCTGGCATATGTGGCTGCT
GGGGATCTGGCACCCATAAACGCCTTCATTGGGGGCCTGGCTGCCCAGGA
AGTCATGAAGGCCTGCTCCGGGAAGTTCATGCCCATCATGCAGTGGCTAT
ACTTTGATGCCCTTGAGTGTCTCCCTGAGGACAAAGAGGTCCTCACAGAG
GACAAGTGCCTCCAGCACCAGAACCGTTATGACGGGCAAGTGGCTGTGTT
TGGCTCAGACCTGCAAGAGAAGCTGGGCAAGCAGAAGTATTTCCTGGTGG
GTGCGGGGGCCATTGGCTGTGAGCTGCTCAAGAACTTTGCCATGATTGGG
CTGGGCTGCGGGGAGGGTGGAGAAATCATCGTTACAGACATGGACACCAT
TGAGAAGTCAAATCTGAATCGACAGTTTCTTTTCCGGCCCTGGGATGTCA
CGAAGTTAAAGTCTGACACGGCTGCTGCAGCTGTGCGCCAAATGAATCCA
CATATCCGGGTGACAAGCCACCAGAACCGTGTGGGTCCTGACACGGAGCG
CATCTATGATGACGATTTTTTCCAAAACCTAGATGGCGTGGCCAATGCCC
TGGACAACGTGGATGCCCGCATGTACATGGACCGCCGCTGTGTCTACTAC
CGGAAGCCACTGCTGGAGTCAGGCACACTGGGCACCAAAGGCAATGTGCA
GGTGGTGATCCCCTTCCTGACAGAGTCGTACAGTTCCAGCCAGGACCCAC
CTGAGAAGTCCATCCCCATCTGTACCCTGAAGAACTTCCCTAATGCCATC
GAGCACACCCTGCAGTGGGCTCGGGATGAGTTTGAAGGCCTCTTCAAGCA
GCCAGCAGAAAATGTCAACCAGTACCTCACAGACCCCAAGTTTGTGGAGC
GAACACTGCGGCTGGCAGGCACTCAGCCCTTGGAGGTGCTGGAGGCTGTG
CAGCGCAGCCTGGTGCTGCAGCGACCACAGACCTGGGCTGACTGCGTGAC
CTGGGCCTGCCACCACTGGCACACCCAGTACTCGAACAACATCCGGCAGC
TGCTGCACAACTTCCCTCCTGACCAGCTCACAAGCTCAGGAGCGCCGTTC
TGGTCTGGGCCCAAACGCTGTCCACACCCGCTCACCTTTGATGTCAACAA
TCCCCTGCATCTGGACTATGTGATGGCTGCTGCCAACCTGTTTGCCCAGA
CCTACGGGCTGACAGGCTCTCAGGACCGAGCTGCTGTGGCCACATTCCTG
CAGTCTGTGCAGGTCCCCGAATTCACCCCCAAGTCTGGCGTCAAGATCCA
TGTTTCTGACCAGGAGCTGCAGAGCGCCAATGCCTCTGTTGATGACAGTC
GTCTAGAGGAGCTCAAAGCCACTCTGCCCAGCCCAGACAAGCTCCCTGGA
TTCAAGATGTACCCCATTGACTTTGAGAAGGATGATGACAGCAACTTTCA
TATGGATTTCATCGTGGCTGCATCCAACCTCCGGGCAGAAAACTATGACA
TTCCTTCTGCAGACCGGCACAAGAGCAAGCTGATTGCAGGGAAGATCATC
CCAGCCATTGCCACGACCACAGCAGCCGTGGTTGGCCTTGTGTGTCTGGA
GCTGTACAAGGTTGTGCAGGGGCACCGACAGCTTGACTCCTACAAGAATG
GTTTCCTCAACTTGGCCCTGCCTTTCTTTGGTTTCTCTGAACCCCTTGCC
GCACCACGTCACCAGTACTATAACCAAGAGTGGACATTGTGGGATCGCTT
TGAGGTACAAGGGCTGCAGCCTAATGGTGAGGAGATGACCCTCAAACAGT
TCCTCGACTATTTTAAGACAGAGCACAAATTAGAGATCACCATGCTGTCC
CAGGGCGTGTCCATGCTCTATTCCTTCTTCATGCCAGCTGCCAAGCTCAA
GGAACGGTTGGATCAGCCGATGACAGAGATTGTGAGCCGTGTGTCGAAGC
GAAAGCTGGGCCGCCACGTGCGGGCGCTGGTGCTTGAGCTGTGCTGTAAC
GACGAGAGCGGCGAGGATGTCGAGGTTCCCTATGTCCGATACACCATCCG
CTGAggatcc

Amino Acid Sequence

MYPYDVPDYAGSAKNGSEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSG
LRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAE
VSQPRLAELNSYVPVTAYTGPLVEDFLSGFQVVVLTNTPLEDQLRVGEFC
HNRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNP
GVVTCLDEARHGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPYTFSICD
TSNFSDYIRGGIVSQVKVPKKISFKSLVASLAEPDFVVTDFAKFSRPAQL
HIGFQALHQFCAQHGRPPRPRNEEDAAELVALAQAVNARALPAVQQNNLD
EDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDAL
ECLPEDKEVLTEDKCLQHQNRYDGQVAVFGSDLQEKLGKQKYFLVGAGAI
GCELLKNFAMIGLGCGEGGEIIVTDMDTIEKSNLNRQFLFRPWDVTKLKS
DTAAAAVRQMNPHIRVTSHQNRVGPDTERIYDDDFFQNLDGVANALDNVD
ARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSI
PICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRL
AGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNF
PPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTYGLT
GSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEEL
KATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPSAD
RHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYKNGFLNL
ALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYF
KTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGR
HVRALVLELCCNDESGEDVEVPYVRYTIR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5765
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Sequence differences compared with X56976 CDS: ; g569a (G190D), c1300g (Q434E)- these changes present in all E1a published sequences for all other species and in other human sequences (e.g. AAP36419, NP_695012) ; g1340a (R447H) – this change present in some mouse E1a sequences and in human EST sequences (e.g. IMAGE consortium EST clones 6537166, 6598339, 6464027).; ; Estimated MW of expressed protein : 115005 Daltons