cDNA Clone E1a

• Plasmid: pCMV5-HA E1a S13A
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-E1a S13A
• Genbank: P22314/X56976
• Tag: HA
• Species: Human

Sequence of Insert

ggatccTCCAGCTCGCCGCTGTCCAAGAAACGTCGCGTGGCCGGGCCTGATCCAAAGCCGGGTTCTAACTGCTCCCCTGCCCAGTCCGTGTTGTCCGAAGTGCCCTCGGTGCCAACCAACGGAATGGCCAAGAACGGCAGTGAAGCAGACATA
GACGAGGGCCTTTACTCCCGGCAGCTGTATGTGTTGGGCCATGAGGCAAT
GAAGCGGCTCCAGACATCCAGTGTCCTGGTATCAGGCCTGCGGGGCCTGG
GCGTGGAGATCGCTAAGAACATCATCCTTGGTGGGGTCAAGGCTGTTACC
CTACATGACCAGGGCACTGCCCAGTGGGCTGATCTTTCCTCCCAGTTCTA
CCTGCGGGAGGAGGACATCGGTAAAAACCGGGCCGAGGTATCACAGCCCC
GCCTCGCTGAGCTCAACAGCTATGTGCCTGTCACTGCCTACACTGGACCC
CTCGTTGAGGACTTCCTTAGTGGTTTCCAGGTGGTGGTGCTCACCAACAC
CCCCCTGGAGGACCAGCTGCGAGTGGGTGAGTTCTGTCACAACCGTGGCA
TCAAGCTGGTGGTGGCAGGCACGCGGGGCCTGTTTGGGCAGCTCTTCTGT
GACTTTGGAGAGGAAATGATCCTCACAGATTCCAATGGGGAGCAGCCACT
CAGTGCTATGGTTTCTATGGTTACCAAGGACAACCCCGGTGTGGTTACCT
GCCTGGATGAGGCCCGACACGGGTTTGAGAGCGGGGACTTTGTCTCCTTT
TCAGAAGTACAGGGCATGGTTGAACTCAACGGAAATCAGCCCATGGAGAT
CAAAGTCCTGGGTCCTTATACCTTTAGCATCTGTGACACCTCCAACTTCT
CCGACTACATCCGTGGAGGCATCGTCAGTCAGGTCAAAGTACCTAAGAAG
ATTAGCTTTAAATCCTTGGTGGCCTCACTGGCAGAACCTGACTTTGTGGT
GACGGACTTCGCCAAGTTTTCTCGCCCTGCCCAGCTGCACATTGGCTTCC
AGGCCCTGCACCAGTTCTGTGCTCAGCATGGCCGGCCACCTCGGCCCCGC
AATGAGGAGGATGCAGCAGAACTGGTAGCCTTAGCACAGGCTGTGAATGC
TCGAGCCCTGCCAGCAGTGCAGCAAAATAACCTGGACGAGGACCTCATCC
GGAAGCTGGCATATGTGGCTGCTGGGGATCTGGCACCCATAAACGCCTTC
ATTGGGGGCCTGGCTGCCCAGGAAGTCATGAAGGCCTGCTCCGGGAAGTT
CATGCCCATCATGCAGTGGCTATACTTTGATGCCCTTGAGTGTCTCCCTC
AGGACAAAGAGGTCCTCACAGAGGACAAGTGCCTCCAGCGCCAGAACCGT
TATGACGGGCAAGTGGCTGTGTTTGGCTCAGACCTGCAAGAGAAGCTGGG
CAAGCAGAAGTATTTCCTGGTGGGTGCGGGGGCCATTGGCTGTGAGCTGC
TCAAGAACTTTGCCATGATTGGGCTGGGCTGCGGGGAGGGTGGAGAAATC
ATCGTTACAGACATGGACACCATTGAGAAGTCAAATCTGAATCGACAGTT
TCTTTTCCGGCCCTGGGATGTCACGAAGTTAAAGTCTGACACGGCTGCTG
CAGCTGTGCGCCAAATGAATCCACATATCCGGGTGACAAGCCACCAGAAC
CGTGTGGGTCCTGACACGGAGCGCATCTATGATGACGATTTTTTCCAAAA
CCTAGATGGCGTGGCCAATGCCCTGGACAACGTGGATGCCCGCATGTACA
TGGACCGCCGCTGTGTCTACTACCGGAAGCCACTGCTGGAGTCAGGCACA
CTGGGCACCAAAGGCAATGTGCAGGTGGTGATCCCCTTCCTGACAGAGTC
GTACAGTTCCAGCCAGGACCCACCTGAGAAGTCCATCCCCATCTGTACCC
TGAAGAACTTCCCTAATGCCATCGAGCACACCCTGCAGTGGGCTCGGGAT
GAGTTTGAAGGCCTCTTCAAGCAGCCAGCAGAAAATGTCAACCAGTACCT
CACAGACCCCAAGTTTGTGGAGCGAACACTGCGGCTGGCAGGCACTCAGC
CCTTGGAGGTGCTGGAGGCTGTGCAGCGCAGCCTGGTGCTGCAGCGACCA
CAGACCTGGGCTGACTGCGTGACCTGGGCCTGCCACCACTGGCACACCCA
GTACTCGAACAACATCCGGCAGCTGCTGCACAACTTCCCTCCTGACCAGC
TCACAAGCTCAGGAGCGCCGTTCTGGTCTGGGCCCAAACGCTGTCCACAC
CCGCTCACCTTTGATGTCAACAATCCCCTGCATCTGGACTATGTGATGGC
TGCTGCCAACCTGTTTGCCCAGACCTACGGGCTGACAGGCTCTCAGGACC
GAGCTGCTGTGGCCACATTCCTGCAGTCTGTGCAGGTCCCCGAATTCACC
CCCAAGTCTGGCGTCAAGATCCATGTTTCTGACCAGGAGCTGCAGAGCGC
CAATGCCTCTGTTGATGACAGTCGTCTAGAGGAGCTCAAAGCCACTCTGC
CCAGCCCAGACAAGCTCCCTGGATTCAAGATGTACCCCATTGACTTTGAG
AAGGATGATGACAGCAACTTTCATATGGATTTCATCGTGGCTGCATCCAA
CCTCCGGGCAGAAAACTATGACATTCCTTCTGCAGACCGGCACAAGAGCA
AGCTGATTGCAGGGAAGATCATCCCAGCCATTGCCACGACCACAGCAGCC
GTGGTTGGCCTTGTGTGTCTGGAGCTGTACAAGGTTGTGCAGGGGCACCG
ACAGCTTGACTCCTACAAGAATGGTTTCCTCAACTTGGCCCTGCCTTTCT
TTGGTTTCTCTGAACCCCTTGCCGCACCACGTCACCAGTACTATAACCAA
GAGTGGACATTGTGGGATCGCTTTGAGGTACAAGGGCTGCAGCCTAATGG
TGAGGAGATGACCCTCAAACAGTTCCTCGACTATTTTAAGACAGAGCACA
AATTAGAGATCACCATGCTGTCCCAGGGCGTGTCCATGCTCTATTCCTTC
TTCATGCCAGCTGCCAAGCTCAAGGAACGGTTGGATCAGCCGATGACAGA
GATTGTGAGCCGTGTGTCGAAGCGAAAGCTGGGCCGCCACGTGCGGGCGC
TGGTGCTTGAGCTGTGCTGTAACGACGAGAGCGGCGAGGATGTCGAGGTT
CCCTATGTCCGATACACCATCCGCTGAggatcc

Amino Acid Sequence

MYPYDVPDYAGSSSSPLSKKRRVAGPDPKPGSNCSPAQSVLSEVPSVPTN
GMAKNGSEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAK
NIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELN
SYVPVTAYTGPLVEDFLSGFQVVVLTNTPLEDQLRVGEFCHNRGIKLVVA
GTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEAR
HGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPYTFSICDTSNFSDYIRG
GIVSQVKVPKKISFKSLVASLAEPDFVVTDFAKFSRPAQLHIGFQALHQF
CAQHGRPPRPRNEEDAAELVALAQAVNARALPAVQQNNLDEDLIRKLAYV
AAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPQDKEVL
TEDKCLQRQNRYDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAM
IGLGCGEGGEIIVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQM
NPHIRVTSHQNRVGPDTERIYDDDFFQNLDGVANALDNVDARMYMDRRCV
YYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPN
AIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLE
AVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGA
PFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVAT
FLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKL
PGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPSADRHKSKLIAGK
IIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYKNGFLNLALPFFGFSEP
LAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITM
LSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELC
CNDESGEDVEVPYVRYTIR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5744
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Sequence differences compared with X56976 CDS: ; g569a (G190D), c1300g (Q434E)- these changes present in all E1a published sequences for all other species and in other human sequences (e.g. AAP36419, NP_695012) ; g1340a (R447H) – this change present in some mouse E1a sequences and in human EST sequences (e.g. IMAGE consortium EST clones 6537166, 6598339, 6464027).; ; ; Estimated MW of expressed protein : 118997 Daltons