cDNA Clone IPS (Barley)

• Plasmid: pEBG6P-1 Hor. v. IPS
• Parent Plasmid: pEBG6P
• Genbank: AF056325
• Species: Hordeum vulgare

Sequence of Insert

GGATCCATGTTCATCGAGAGCTTCCGCGTGGAGAGCCCGAAGGTGCGGTACGGCGCGGGGGAGATCGAGTCCGAGTACCGCTACGACACCACGGAGCTGGTGCACGAGAGCCACGACGGCGCCTCCAAGTGGGTCGTCCGCCCCAAGTCCGTCAACTACCACTTCAAGACCAACACCACCGTCCCCAAGCTCGGGGTGATGCTCGTGGGGTGGGGCGGCAACAATGGCTCCACACTCATGGCTGGGGTCATCGCCAACAGGGAGGGGATCTCATGGGCGACAAAGGACAAGGTGCAGCAGGCCAAC
TACTTTGGGTCCCTCACCCAGGCCTCCACCATCAGGGTCGGGAGCTACAA
CGGAGAGGAGATCTATGCGCCCTTCAAGAGCCTCCTTCCCATGGTGAATC
CGGATGACCTTGTGTTCGGGGGCTGGGACATTAGCAGCATGAACCTGGCT
GATGCTATGACCAGGGCCAAGGTGCTGGACATTGACCTGCAGAAGCAGCT
CAGGCCCTATATGGAGTCCATCGTGCCGCTCCCTGGCATCTATGACCCGG
ACTTCATCGCTGCCAACCAGGGCTCCCGGGCTAACAATGTCATCAAGGGC
ACAAAGAAAGAGCAGATGGAGCAGGTCATCAAAGACATAAGGGAGTTCAA
GGAGAAGAACAAGGTAGACAAGGTAGTGGTGCTCTGGACGGCAAACACTG
AAAGGTACAGCAACGTCTCTGTTGGGCTTAATGACACGACTGAGAACCTC
TTGGCGTCTGTGGACAAGAACGAGGCTGAGATATCTCCATCAACACTGTA
TGCCATTGCCTGTGTCATGGAGGGTGTGCCGTTCATCAATGGGAGCCCTC
AGAACACCTTTGTGCCTGGACTGATTGATCTTGCTATTAAGAATGACTGC
CTGATTGGTGGCGATGATTTCAAGAGTGGCCAGACCAAGATGAAGTCTGT
CTTGGTCGACTTCCTTGTTGGTGCTGGAATCAAGCCCACCTCAATTGTCA
GCTACAACCATCTGGGGAACAACGACGGGATGAACCTATCCGCACCGCAA
ACATTTCGTTCCAAGGAGATCTCAAAGAGCAACGTGGTGGATGACATGGT
CTCAAGCAATGCTATCCTCTATGAGCCCGGGGAACATCCTGACCACGTCG
TCGTGATCAAGTACGTGCCATACGTTGGAGACAGCAAGAGGGCCATGGAC
GAGTATACCTCGGAGATCTTCATGGGGGGCAAGAGCACCATCGTGCTGCA
CAACACCTGCGAGGACTCGCTCCTCGCCGCGCCGATCATTCTTGATTTGG
TGCTCCTGGCCGAGCTCAGCACCAGGATTCAGCTGAAAGCCGAGGGAGAG
GACAAGTTCCATTCCTTCCATCCGGTTGCCACCATCCTGAGCTACCTCAC
CAAGGCGCCCCTGGTCCCTCCAGGCACACCGGTGGTGAACGCGCTGGCGA
AGCAGAGGGCGATGCTGGAGAACATCATGAGGGCGTGCGTCGGCCTGGCG
CCCGAGAACAACATGATCCTGGAGTACAAGTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMFIESFRVESPKV
RYGAGEIESEYRYDTTELVHESHDGASKWVVRPKSVNYHFKTNTTVPKLG
VMLVGWGGNNGSTLMAGVIANREGISWATKDKVQQANYFGSLTQASTIRV
GSYNGEEIYAPFKSLLPMVNPDDLVFGGWDISSMNLADAMTRAKVLDIDL
QKQLRPYMESIVPLPGIYDPDFIAANQGSRANNVIKGTKKEQMEQVIKDI
REFKEKNKVDKVVVLWTANTERYSNVSVGLNDTTENLLASVDKNEAEISP
STLYAIACVMEGVPFINGSPQNTFVPGLIDLAIKNDCLIGGDDFKSGQTK
MKSVLVDFLVGAGIKPTSIVSYNHLGNNDGMNLSAPQTFRSKEISKSNVV
DDMVSSNAILYEPGEHPDHVVVIKYVPYVGDSKRAMDEYTSEIFMGGKST
IVLHNTCEDSLLAAPIILDLVLLAELSTRIQLKAEGEDKFHSFHPVATIL
SYLTKAPLVPPGTPVVNALAKQRAMLENIMRACVGLAPENNMILEYK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5694
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express Hor. v. IPS with N-terminal GST (contains preScission protease site); N.B. Sequence differences compared with AF056325:; c1357t (L453F), c1276g/g1277c (R426A), gtgc522cgtg (M174I/C175V), a561t (silent A187), t636c (silent D212); ; Estimated MW of expressed protein : 83515 Daltons