cDNA Clone MEKK2 (mouse)

• Synonyms: Map3k2
• Plasmid: pEBG6P-1 mMEKK2 K385M
• Parent Plasmid: pEBG6P
• Expressed: GST-MEKK2 K385M
• Genbank: NP_036076/ NM_011946
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCGATGGATGATCAGCAAGCTTTGAATTCAATCATGCAAGATTTGGCTGTCCTTCATAAGGCCAGTCGGCCAGCATTATCTTTACAAGAAACCAGGAAAGCAAAACCTTCATCACCAAAAAAACAGAATGATGTTCGAGTCAAATTTGAACATAGAGGAGAAAAAAGGATCCTGCAGGTTACTAGACCAGTTAAACTAGAAGACCTGAGATCTAAGTCTAAGATCGCCTTTGGGCAGTCTATGGATCTACACTATACCAACAATGAGTTGGTAATTCCGTTAACTACCCAAGATGACTTGGACAAAGCTGTGGAACTGCTGGATCGCAGTATTCACATGAAGAGTCTCAAGATATTACTTGTAGTAAATGGGAGTACACAGGCTACTAATTTAGAACCATCGCCGTCACCAGAAGATTTGAATAATACACCACTTGGTGCAGAGAGGAAAAAG
CGGCTATCTGTAGTAGGTCCCCCTAATAGGGATAGAAGTTCCCCTCCTCC
AGGATACATTCCAGACGAGCTACACCAGATTGCCCGGAATGGGTCATTCA
CTAGCATCAACAGTGAAGGAGAGTTCATTCCAGAGAGCATGGACCAAATG
CTGGATCCATTGTCTTTAAGCAGCCCTGAAAATTCTGGCTCAGGAAGCTG
TCCGTCACTTGATAGTCCTTTGGATGGAGAAAGCTACCCAAAATCACGGA
TGCCTAGGGCACAGAGCTACCCAGATAATCATCAGGAGTTTACAGACTAT
GATAACCCCATTTTTGAGAAATTTGGAAAAGGAGGAACATATCCAAGAAG
GTACCACGTTTCCTATCATCACCAGGAGTATAATGACGGTCGGAAGACTT
TTCCAAGAGCTAGAAGGACCCAGGGCACCAGTTTCCGGTCTCCTGTGAGC
TTCAGTCCTACTGATCACTCCTTAAGCACTAGTAGTGGAAGCAGTGTCTT
TACCCCAGAGTATGACGACAGTCGAATAAGAAGACGGGGGAGTGACATAG
ACAATCCTACTTTGACTGTCACAGACATCAGCCCACCCAGCCGTTCACCT
CGAGCTCCGACCAACTGGAGACTGGGCAAGCTGCTTGGCCAAGGAGCTTT
TGGTAGGGTCTACCTCTGCTATGATGTTGATACCGGAAGAGAGCTGGCTG
TTATGCAAGTTCAGTTTAACCCTGAGAGCCCAGAGACCAGCAAGGAAGTA
AATGCACTTGAGTGTGAAATTCAGTTGTTGAAAAACTTGTTGCATGAGCG
AATTGTTCAGTATTATGGCTGTTTGAGGGATCCTCAGGAGAAAACACTTT
CCATCTTTATGGAGTATATGCCAGGGGGTTCAATTAAGGACCAACTAAAA
GCCTACGGAGCTCTTACTGAGAACGTGACGAGGAAGTACACCCGTCAGAT
TCTGGAGGGGGTCCATTATTTGCATAGTAATATGATTGTCCATAGAGATA
TCAAAGGAGCAAATATCTTAAGGGATTCCACAGGCAATATCAAGTTAGGA
GACTTTGGGGCTAGTAAACGGCTTCAGACCATCTGTCTCTCAGGCACAGG
AATGAAGTCTGTCACAGGCACGCCATACTGGATGAGTCCTGAGGTCATCA
GTGGAGAAGGCTATGGAAGAAAAGCAGACATCTGGAGCGTAGCATGTACT
GTGGTAGAAATGCTAACTGAAAAGCCACCTTGGGCTGAATTTGAAGCAAT
GGCTGCCATCTTTAAGATCGCCACTCAGCCAACGAACCCAAAGCTGCCAC
CTCATGTCTCAGACTATACTCGGGACTTCCTCAAACGGATTTTTGTAGAG
GCCAAACTTCGACCTTCAGCGGAGGAGCTCTTGCGGCACATGTTTGTGCA
TTATCACTAGGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMDDQ
QALNSIMQDLAVLHKASRPALSLQETRKAKPSSPKKQNDVRVKFEHRGEK
RILQVTRPVKLEDLRSKSKIAFGQSMDLHYTNNELVIPLTTQDDLDKAVE
LLDRSIHMKSLKILLVVNGSTQATNLEPSPSPEDLNNTPLGAERKKRLSV
VGPPNRDRSSPPPGYIPDELHQIARNGSFTSINSEGEFIPESMDQMLDPL
SLSSPENSGSGSCPSLDSPLDGESYPKSRMPRAQSYPDNHQEFTDYDNPI
FEKFGKGGTYPRRYHVSYHHQEYNDGRKTFPRARRTQGTSFRSPVSFSPT
DHSLSTSSGSSVFTPEYDDSRIRRRGSDIDNPTLTVTDISPPSRSPRAPT
NWRLGKLLGQGAFGRVYLCYDVDTGRELAVMQVQFNPESPETSKEVNALE
CEIQLLKNLLHERIVQYYGCLRDPQEKTLSIFMEYMPGGSIKDQLKAYGA
LTENVTRKYTRQILEGVHYLHSNMIVHRDIKGANILRDSTGNIKLGDFGA
SKRLQTICLSGTGMKSVTGTPYWMSPEVISGEGYGRKADIWSVACTVVEM
LTEKPPWAEFEAMAAIFKIATQPTNPKLPPHVSDYTRDFLKRIFVEAKLR
PSAEELLRHMFVHYH*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5669
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express mouse MEKK2 K385M with N-terminal GST (contains preScission protease site); N.B. Nucleotide sequence differences compared with NM_011946:; A399g (silent); T1638c (silent); ; Estimated MW of expressed protein : 97890 Daltons