cDNA Clone MEKK2 (mouse)

• Synonyms: Map3k2
• Plasmid: pCMV5-HA3 mMEKK2 K385M
• Parent Plasmid: pCMV5 HA3
• Expressed: HA-MEKK2 K385M
• Genbank: NP_036076
• Tag: HA
• Species: Mouse

Sequence of Insert

gtcgacatggatgatcagcaagctttgaattcaatcatgcaagatttggctgtccttcataaggccagtcggccagcattatctttacaagaaaccaggaaagcaaaaccttcatcaccaaaaaaacagaatgatgttcgagtcaaatttgaacatagaggagaaaaaaggatcctgcaggttactagaccagttaaactagaagacctgagatctaagtctaagatcgcctttgggcagtctatggatctacactataccaacaatgagttggtaattccgttaactacccaagatgacttggac
aaagctgtggaactgctggatcgcagtattcacatgaagagtctcaagat
attacttgtagtaaatgggagtacacaggctactaatttagaaccatcac
cgtcaccagaagatttgaataatacaccacttggtgcagagaggaaaaag
cggctatctgtagtaggtccccctaatagggatagaagttcccctcctcc
aggatacattccagacgagctacaccagattgcccggaatgggtcattca
ctagcatcaacagtgaaggagagttcattccagagagcatggaccaaatg
ctggatccattgtctttaagcagccctgaaaattctggctcaggaagctg
tccgtcacttgatagtcctttggatggagaaagctacccaaaatcacgga
tgcctagggcacagagctacccagataatcatcaggagtttacagactat
gataaccccatttttgagaaatttggaaaaggaggaacatatccaagaag
gtaccacgtttcctatcatcaccaggagtataatgacggtcggaagactt
ttccaagagctagaaggacccagggcaccagtttccggtctcctgtgagc
ttcagtcctactgatcactccttaagcactagtagtggaagcagtgtctt
taccccagagtatgacgacagtcgaataagaagacgggggagtgacatag
acaatcctactttgactgtcacagacatcagcccacccagccgttcacct
cgagctccgaccaactggagactgggcaagctgcttggccaaggagcttt
tggtagggtctacctctgctatgatgttgataccggaagagagctggctg
ttatgcaagttcagtttaaccctgagagcccagagaccagcaaggaagta
aatgcacttgagtgtgaaattcagttgttgaaaaacttgttgcatgagcg
aattgttcagtattatggctgtttgagggatcctcaggagaaaacacttt
ccatctttatggagtatatgccagggggttcaattaaggaccaactaaaa
gcctacggagctcttactgagaacgtgacgaggaagtacacccgtcagat
tctggagggggtccattatttgcatagtaatatgattgtccatagagata
tcaaaggagcaaatatcttaagggattccacaggcaatatcaagttagga
gactttggggctagtaaacggcttcagaccatctgtctctcaggcacagg
aatgaagtctgtcacaggcacgccatactggatgagtcctgaggtcatca
gtggagaaggctatggaagaaaagcagacatctggagtgtagcatgtact
gtggtagaaatgctaactgaaaagccaccttgggctgaatttgaagcaat
ggctgccatctttaagatcgccactcagccaacgaacccaaagctgccac
ctcatgtctcagactatactcgggacttcctcaaacggatttttgtagag
gccaaacttcgaccttcagcggaggagctcttgcggcacatgtttgtgca
ttatcactaggcggccgc

Amino Acid Sequence

MYPYDVPDYAGDPRNSRVDMDDQQALNSIMQDLAVLHKASRPALSLQETR
KAKPSSPKKQNDVRVKFEHRGEKRILQVTRPVKLEDLRSKSKIAFGQSMD
LHYTNNELVIPLTTQDDLDKAVELLDRSIHMKSLKILLVVNGSTQATNLE
PSPSPEDLNNTPLGAERKKRLSVVGPPNRDRSSPPPGYIPDELHQIARNG
SFTSINSEGEFIPESMDQMLDPLSLSSPENSGSGSCPSLDSPLDGESYPK
SRMPRAQSYPDNHQEFTDYDNPIFEKFGKGGTYPRRYHVSYHHQEYNDGR
KTFPRARRTQGTSFRSPVSFSPTDHSLSTSSGSSVFTPEYDDSRIRRRGS
DIDNPTLTVTDISPPSRSPRAPTNWRLGKLLGQGAFGRVYLCYDVDTGRE
LAVMQVQFNPESPETSKEVNALECEIQLLKNLLHERIVQYYGCLRDPQEK
TLSIFMEYMPGGSIKDQLKAYGALTENVTRKYTRQILEGVHYLHSNMIVH
RDIKGANILRDSTGNIKLGDFGASKRLQTICLSGTGMKSVTGTPYWMSPE
VISGEGYGRKADIWSVACTVVEMLTEKPPWAEFEAMAAIFKIATQPTNPK
LPPHVSDYTRDFLKRIFVEAKLRPSAEELLRHMFVHYH*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5653
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 71897 Daltons