cDNA Clone MEKK2 (mouse)

• Synonyms: Map3k2
• Plasmid: pEBG6P-1 mMEKK2
• Parent Plasmid: pEBG6P
• Expressed: GST-MEKK2
• Genbank: NM_011946
• Tag: GST
• Species: Mouse

Sequence of Insert

gcggccgcgatggatgatcagcaagctttgaattcaatcatgcaagatttggctgtccttcataaggccagtcggccagcattatctttacaagaaaccaggaaagcaaaaccttcatcaccaaaaaaacagaatgatgttcgagtcaaatttgaacatagaggagaaaaaaggatcctgcaggttactagaccagttaaactagaagacctgagatctaagtctaagatcgcctttgggcagtctatggatctacactataccaacaatgagttggtaattccgttaactacccaagatgacttggacaaagctgtggaactgctggatcgcagtattcacatgaagagtctcaagatattacttgtagtaaatgggagtacacaggctactaatttagaaccatcaccgtcaccagaagatttgaataatacaccacttggtgcagagaggaaaaag
cggctatctgtagtaggtccccctaatagggatagaagttcccctcctcc
aggatacattccagacgagctacaccagattgcccggaatgggtcattca
ctagcatcaacagtgaaggagagttcattccagagagcatggaccaaatg
ctggatccattgtctttaagcagccctgaaaattctggctcaggaagctg
tccgtcacttgatagtcctttggatggagaaagctacccaaaatcacgga
tgcctagggcacagagctacccagataatcatcaggagtttacagactat
gataaccccatttttgagaaatttggaaaaggaggaacatatccaagaag
gtaccacgtttcctatcatcaccaggagtataatgacggtcggaagactt
ttccaagagctagaaggacccagggcaccagtttccggtctcctgtgagc
ttcagtcctactgatcactccttaagcactagtagtggaagcagtgtctt
taccccagagtatgacgacagtcgaataagaagacgggggagtgacatag
acaatcctactttgactgtcacagacatcagcccacccagccgttcacct
cgagctccgaccaactggagactgggcaagctgcttggccaaggagcttt
tggtagggtctacctctgctatgatgttgataccggaagagagctggctg
ttaagcaagttcagtttaaccctgagagcccagagaccagcaaggaagta
aatgcacttgagtgtgaaattcagttgttgaaaaacttgttgcatgagcg
aattgttcagtattatggctgtttgagggatcctcaggagaaaacacttt
ccatctttatggagtatatgccagggggttcaattaaggaccaactaaaa
gcctacggagctcttactgagaacgtgacgaggaagtacacccgtcagat
tctggagggggtccattatttgcatagtaatatgattgtccatagagata
tcaaaggagcaaatatcttaagggattccacaggcaatatcaagttagga
gactttggggctagtaaacggcttcagaccatctgtctctcaggcacagg
aatgaagtctgtcacaggcacgccatactggatgagtcctgaggtcatca
gtggagaaggctatggaagaaaagcagacatctggagtgtagcatgtact
gtggtagaaatgctaactgaaaagccaccttgggctgaatttgaagcaat
ggctgccatctttaagatcgccactcagccaacgaacccaaagctgccac
ctcatgtctcagactatactcgggacttcctcaaacggatttttgtagag
gccaaacttcgaccttcagcggaggagctcttgcggcacatgtttgtgca
ttatcactaggcggccgc

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMDDQ
QALNSIMQDLAVLHKASRPALSLQETRKAKPSSPKKQNDVRVKFEHRGEK
RILQVTRPVKLEDLRSKSKIAFGQSMDLHYTNNELVIPLTTQDDLDKAVE
LLDRSIHMKSLKILLVVNGSTQATNLEPSPSPEDLNNTPLGAERKKRLSV
VGPPNRDRSSPPPGYIPDELHQIARNGSFTSINSEGEFIPESMDQMLDPL
SLSSPENSGSGSCPSLDSPLDGESYPKSRMPRAQSYPDNHQEFTDYDNPI
FEKFGKGGTYPRRYHVSYHHQEYNDGRKTFPRARRTQGTSFRSPVSFSPT
DHSLSTSSGSSVFTPEYDDSRIRRRGSDIDNPTLTVTDISPPSRSPRAPT
NWRLGKLLGQGAFGRVYLCYDVDTGRELAVKQVQFNPESPETSKEVNALE
CEIQLLKNLLHERIVQYYGCLRDPQEKTLSIFMEYMPGGSIKDQLKAYGA
LTENVTRKYTRQILEGVHYLHSNMIVHRDIKGANILRDSTGNIKLGDFGA
SKRLQTICLSGTGMKSVTGTPYWMSPEVISGEGYGRKADIWSVACTVVEM
LTEKPPWAEFEAMAAIFKIATQPTNPKLPPHVSDYTRDFLKRIFVEAKLR
PSAEELLRHMFVHYH*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5646
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express mouse MEKK2 with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 97887 Daltons